国产发酵罐操作说明(英文)

Operating instruction for BIOXING bioreactor

The components for the bioreactor

1-variable area flowmeter:used for regulate the flow rate of air

2-the bioreactor: the place for cultivating strains and fermentation

3-the base:for supporting the bioreactor and alkali bottle

4-feeding pipe and alkali(acid) pipe: for added medium and alkali or acid

5-condenser: used for cooling the out air

6-air outlet: for air exhausting

7-rotator: install the electrical machine for driving the rotator

8-inoculate: adding strain, medium, glucose and other needed for fermentation

9-use for installing pH electrode

10-used for installing oxygen electrode

11-used for installing temperature electrode

12-used for installing the pipes for water used for keeping the temperature at a set point

13-sampling: used for taking samples from bioreactor

14-nuts: for fix the lid and make sure the bioreactor is gas tightness 15-air inlet: for pumping aseptic air to bioreactor

16-parameters sleet

17-user loading in(or out)

18-electrical machine: for driving the rotator

19-temperature electrode

20-bioreactor switch

The reactor(contain at least 1.5L water in it) must be sterilized at 121℃for 30min before used for fermentation, after this, the reactor will be able to use for fermentation.

Instructions for bioreactor use

1.Turn on the power and login the system

User name: SYS Password: 333

2.After loading successfully, some necessary steps must be done before the

fermentation, this may conclude the followings.

A.Electrode calibration: the electrodes used for fermentation are pH and oxygen

electrode in our lab and the calibration for them is similar.

pH: use the standard buffer(7.0,green and 4.0,pink) to calibrate the electrode: pH 7.0 is first and then 4.0

Oxygen: use the saturated sodium sulfite calibrate the zero point and before inoculate the 100% point must be calibrate

B.Feeding pump calibration: as the process of fermentation is continued, extra

fresh and aseptic fermentation medium may be added under a constant rate, so the feeding pump must be calibration as you calculated.

3.Bioreactor wrapping and fermentation medium

The fermentation medium is added to bioreactor and sterilized at 121℃for 15min.Before this, following things must be done.

A.Bioreactor assembling: after all the things above are done, first we must assemble

the bioreactor, there may have some points need to be considered. First the seal ring under the lid must be added correctly and four nuts must be screwed. Another one is the direction of the condenser, it must be placed different side compared with the scale in the glass.

B.Bioreactor wrapping: in order to ensure the aseptic environment for fermentation,

the wrapping is necessary: first, the pipes for taking samples, air output and medium feeding must be wrapped by silver paper or gauze (eight layer); and then two air filter must be added at the entrance of pipe for air input (notice the direction, the inlet is faced outside) and then wrapped by silver paper.

C.Fermentation medium preparation: weighing all the chemical reagents and

dissolved by tap water. In this step, the water added for regents dissolving is not