两个新的水稻叶色突变体形态结构与遗传定位研究

中国农业科学 2010,43(2):223-229

Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.02.001

两个新的水稻叶色突变体形态结构与遗传定位研究

张力科1，李志彬2，刘海燕1，李如海2，陈满元1，陈爱国2，钱益亮1，华泽田2， 高用明1，

朱苓华1，黎志康1,3

（1中国农业科学院作物科学研究所，北京 100081；2辽宁省稻作研究所，沈阳 110101；3 International Rice Research Institute, DAPO Box7777,

Metro Manila, Philippines）

摘要：【目的】对2个新的水稻叶色突变体进行形态结构与遗传分析，并且初步定位这2个突变基因。【方法】在水稻育种材料中分别发现了一株白色条纹叶突变体和一株黄叶突变体，经多代自交已形成稳定的突变系。对突变体的主要形态特征与叶绿素组分等进行分析，观察叶绿体的超微结构，并以这2个突变系杂交产生的F2群体作为定位群体，应用SSR标记对突变基因进行初定位。【结果】与其野生型相比，白色条纹叶突变体的单株穗数减少

12.86%，生育期延长11.27%，黄色叶突变体的株高降低31.08%，千粒重减少14.55%，生育期延长17.86%，并且

2种突变体的叶绿素含量都显著低于其野生型。电镜观察结果表明：2种突变体的类囊体结构异常，与野生型水稻相比，黄色叶突变体的类囊体片层数变少，白色条纹叶中条纹部分的类囊体片层结构几乎消失，正常绿色部分的类囊体结构没有变化。遗传分析表明：这2种突变性状均受1对隐性核基因控制，位于不同染色体上，将突变基因暂时命名为st9(t)(stripe)、chl12(t) (chlorophyll-deficit)。将st9(t)定位到第一染色体短臂最末端，与分子标记RM1331相距9.6 cM，且与标记RM3252等共分离；将chl12(t)定位到第三染色体短臂，与分子标记RM411、RM8208之间的遗传距离分别是1.2、5.1 cM。【结论】发现了2个叶色突变新基因，为下一步的基因克隆与功能分析奠定了基础。

关键词：水稻；白条叶突变体；黄色叶突变体；遗传分析；基因定位

Study on Morphological Structure and Genetic Mapping of Two

Novel Leaf Color Mutants in Rice

ZHANG Li-ke1, LI Zhi-bin2, LIU Hai-yan1, LI Ru-hai2, CHEN Man-yuan1, CHEN Ai-guo2, QIAN Yi-liang1, HUA Ze-tian2, GAO Yong-ming1, ZHU Ling-hua1, LI Zhi-kang1,3

(1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 2Liaoning Rice Research Institute, Shenyang 110101; 3International Rice Research Institute, DAPO Box7777, Metro Manila, Philippines)

Abstract: 【Objective】 The objective of this study is to analyze the morphological structure, chlorophyll components, chloroplast ultrastruture and map genes underlying the two novel leaf color mutants in rice. 【Method】A white stripe leaf mutant and a yellow leaf mutant were found in rice breeding materials. The main agronomic character and chloroplast ultrastructure of the mutants were observed. The mutant genes was mapped with SSR markers and F2 mapping population of the cross between the two mutant lines. 【Result】Compared to their wild-type, the panicles per plant of the white stripe leaf mutant decreased by 12.86%, and the growth duration increased by 11.27%; the plant height of yellow leaf mutant decreased by 31.08% and the growth duration increased by 17.86%, and the photosynthetic pigment contents of both mutants were significantly lower than their wild-type. The result of electron microscope observation showed that the grana structures were unnormal in both mutants. The genetic analysis indicated that the white stripe leaf mutant was controlled by a recessive nuclear gene located on the tip of the short arm of rice

收稿日期：2009-05-15；接受日期：2009-06-12

基金项目：国际先进农业科学技术计划（“948”计划）项目（2006-G1(A)）

作者简介：张力科，硕士研究生。共同第一作者李志彬，硕士研究生。通信作者高用明，研究员，博士。E-mail：irriygao@。华泽田，研究员，博士。E-mail：hzetian@