盐酸曲美他嗪

Ultrasonic microdialysis coupled with capillary electrophoresis electrochemiluminescence study the interaction between trimetazidine dihydrochloride and human serum albumin

Shuangjiao Sun,Chanjuan Long,Chunyao Tao,Sa Meng,Biyang Deng *

Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China),School of Chemistry and Pharmaceutical Sciences,Guangxi Normal University,Guilin 541004,China

H I G H L I G H T S

G R A P H I C A L A B S T R A C T

A homemade ultrasonic (US)micro-dialysis (MD)device was described. The equilibrium speed was improved about 6times using USMD.

The binding speed was improved 4times using US technique.

New method for the determination of binding sites number and binding constant was

developed.

A R T I C L E I N F O

Article history:

Received 12June 2014

Received in revised form 1August 2014Accepted 6August 2014

Available online 13August 2014

Keywords:

Ultrasonic microdialysis Capillary electrophoresis Electrochemiluminescence Trimetazidine dihydrochloride Human serum albumin

A B S T R A C T

The paper describes a homemade ultrasonic microdialysis device coupled with capillary electrophoresis electrochemiluminescence (CE-ECL)for studying the interaction between human serum albumin (HSA)and trimetazidine dihydrochloride (TMZ).The time required for equilibrium by ultrasonic microdialysis was 45min,which was far less than that by traditional dialysis (240min).It took 80min to achieve the required combination equilibrium by normal incubation and only 20min by pared with traditional dialysis,the use of ultrasonic microdialysis simpli fied experimental procedures,shortened experimental time and saved consumption of sample.A simple,sensitive and selective determination of TMZ was developed using CE-ECL and the parameters that affected ECL intensity were optimized.Under the optimized conditions,the linear range of TMZ was from 0.075to 80m mol/L (r 2=0.9974).The detection limit was 26nmol/L with RSD of 2.8%.The number of binding sites and binding constant were 1.54and 15.17L/mol,respectively.

ã2014Elsevier B.V.All rights reserved.

1.Introduction

Trimetazidine dihydrochloride (TMZ,Fig.S1in Supporting Information)is an effective and well-tolerated anti-anginal meta-bolic agent used for optimizing myocardial energy metabolism,protecting the adenosine triphosphate generation process,reducing the intracellular acidosis,preventing the accumulation of sodium

and calcium and maintaining stable environment in the cell.Meanwhile,it is an antiadrenergic drug that can reduce vascular resistance,increase blood flow of coronary artery and improve heart function.It also has a curative effect in the treatment of angina pectoris,acute myocardial infarction,ischemic heart disease,idiopathic dilated heart,heart failure,arrhythmia and other diseases [1–8].The increasing interests in metabolic modulation in recent years need to develop quick analytical methods for the determina-tion of TMZ.

Supplementry material related to this article found,in the online version,at /10.1016/j.aca.2014.08.012.

*Corresponding author.Tel.:+867735845726;fax:+867732120958.E-mail address:dengby16@ (B.Deng).

/10.1016/j.aca.2014.08.012

0003-2670/ã2014Elsevier B.V.All rights reserved.

Analytica Chimica Acta 851(2014)37–42

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