四氯化碳综合法制备大鼠肝硬化模型_叶春华

·论著·四氯化碳综合法制备大鼠肝硬化模型

叶春华　刘浔阳

(中南大学湘雅三医院普外科,湖南　长沙　410013)

[摘要]【目的】探讨建立一种理想的大鼠肝硬化模型的复制方法并加以评价,为下一步探索肝硬化门脉

高压症治疗方法提供可靠、稳定的模型动物。【方法】90只雄性SD大鼠随机分为两组:实验组(70只)采用四

氯化碳(CCl4)+苯巴比妥钠+食用白酒进行诱导,诱导期间CCl4浓度在一定范围内逐步增加,并用食用白酒

替代医用乙醇溶液;对照组(20只)给与普通饮水和饲料,频度及剂量同实验组。同时采用一系列的检查方

法加以评估。【结果】实验组70只大鼠造模结束时,死亡9只,死亡率12.86%。实验结束时,按照成模标准,

造模成功大鼠共61只,均可观察到肝硬化结节及镜下假小叶形成,成模率87.14%。实验组门静脉压力比对

照组明显增高(P<0.05);血清总胆红素、谷丙转氨酶及谷草转氨酶较正常对照组明显升高(P<0.05),而

血清白蛋白较之正常对照组明显降低(P<0.05)。对照组20只全部存活。【结论】该方法可形成稳定的肝

硬化门脉高压症模型,可用于肝硬化门脉高压症方面的相关研究。此模型制作周期短(仅需9周),成模率高,

死亡率低,具有简便、快捷、高效的优点,适于大批量制作。

[关键词]　肝硬化,实验性;　疾病模型,动物;　大鼠;　四氯化碳

Study on Method of Inducing Hepatic Cirrhosis Model in Rats by Carbon Tetrachloride

　Y E Chun-hua,　LIU X un-yang(Department o f General Surgery,the T hird X iangy a Hosp ital,Central

South University,Changsha,410013,China)

[Abstract]【Objective】T o dev elo p and evalua te an ideal means to induce cir rhotic model in rats for nex t

study o n live r cir rhosis with po r tal hypertension trea tment.【M ethods】N inety male SD rats we re randomly di-

vided into two gr oups,T he e xperiment g roup(n=70)wa s treated with CCl4,PBS and ea table alcoho l and the

co ncentra tion of CCl4and eatable alcohol were increased stage by stag e in a ce rtain range during the ex peri-

ment.T he co nt rol g roup(n=20)wa s trea ted w ith common drinking w ater and animal feeds,the f requency

and do se w ere the same a s the experime nt g ro up,meanwhile evalua ted by a se ries of check methods.【Results】

Af te r being induced w ith above means,the ra t mor tality of the ex periment gr oup w as12.86%,and the fo rma-

tion rate of liver cir rhosis false lobe was87.14%.T he po rtal v eno us pressure of the e xperiment g roup w as

higher than the co ntrol g ro up(<0.05),ST B,G PT,G O T we re higher than the co ntro l g roup,but SA B de-

crea sed(P<0.05).T he contro l g r oup w as all surv iv al.【Co nclusio n】T he pro cedure is fair ly stable and relia-

ble,the model is useful for resea rch in liv er cir rhosis w ith po rtal hy pe rtension.T he cy cle time of inducing cir-

rhosis model is shor t the mor ta lity is low.

[Key words]　liver cir rho sis,ex pe rimental;　disesase models,animal;　rats;　carbon tetr achloride

[中图分类号]　R575.2　[文献标识码]　A　[文章编号]　1671-7171(2005)05-0619-04

由于病毒性肝炎、酒精性肝病等原因造成的慢性肝病和肝硬化患者有增多的趋势,近十年来有关门脉高压症的研究成为消化学界的热点。本实验通

[作者简介]　叶春华(1967-),男,湖南安乡人,博士,主治医师,主要从事肝胆外科临床工作。

[收稿日期]　2005-04-01过腹腔内注射四氯化碳(CCl4)加饮用苯巴比妥钠和食用酒精,探讨建立一种理想的肝硬化模型的方法。1　材料与方法

1.1　材料

1.1.1　实验动物　90只Sprag ue-Daw ley鼠(简称SD大鼠),全部雄性,体重180～220g,普通大鼠饲