肺积方抗小鼠肺癌转移

肺积方抗小鼠肺癌转移

作者：田建辉, 施志明，周之毅, 黄云胜, 王琳

【摘要】目的：观察肺积方对小鼠肺癌转移的疗效。方法：采用小鼠转移瘤模型，24只C57BL/6小鼠分为模型组、顺铂组和肺积方组，每组8只。模型组以生理盐水0.3 ml灌胃，肺积方组予肺积方煎剂（4 g/ml）0.3 ml灌胃，1次/d，顺铂组予顺铂（0.5 mg/ml）0.52 ml腹腔注射，1次/3 d。干预20 d后，称取小鼠体质量、瘤质量和肺质量，玻片计数法计数肺部转移灶的数目。结果：顺铂组小鼠的体质量低于模型组和肺积方组（P＜0.01），而肺积方组体质量最大；顺铂组和肺积方组的瘤质量低于模型组（P＜0.01）；顺铂组和肺积方组小鼠肺部转移灶数目比模型组少（P＜0.01）。结论：肺积方可以抑制肿瘤生长，减少肺部转移瘤的个数，并具有保持小鼠体质量的作用。

【关键词】肺癌转移中医药肺积方小鼠

Methods: The lung cancer metastasis model of mice was established in this experiment study. Twenty four mice were randomly divided into three groups: untreated group, cisplatin group and Feiji Formula group. Mice in the Feiji Formula group were treated with Feiji Formula decoction; in cisplatin group, with cisplatin by intraperitoneal injection; and in the untreated group, with normal saline (NS). After twenty day treatment, the body and tumor weights as well as the number of metastatic tumors in both lungs of each mouse were measured.

Results: The body weight of mice in cisplatin group was significantly less than that of Feiji Formula group and untreated group (P＜0.01); the tumor weight of mice in cisplatin group and Feiji Formula group was markedly lower than that of untreated group (P＜0.01); and the number of metastatic tumors in cisplatin group and Feiji Formula group was markedly lower than that of the untreated group (P＜0.01), no significant difference between the Feiji Formula group and cisplatin group in terms of the weights and the numbers of metastatic tumors in bilateral lungs.

Conclusion: Feiji Formula can suppress tumor growth and decrease the number of lung metastatic tnmors in the mice, and maintain the body weight of the mice.

Keywords: lung cancer; metastasis; traditional Chinese medicine; Feiji Formula; mice

肺积方是上海中医药大学龙华医院施志明教授的经验方，多年来在临床用于治疗肺癌，取得良好的效果。临床研究证实，肺积方可稳定瘤体，改善中晚期非小细胞肺癌患者的临床症状，提高生存质量；机制研究表明肺积方可以通过多途径干预肺癌免疫逃逸,恢复机体的免疫监视作用，提高临床疗效［13］。为进一步明确肺积方的作用，本研究采用小鼠转移瘤模型，观察肺积方的抑瘤作用和抗肺癌转移的作用。

1 材料和方法

1.1 实验材料

1.1.1 实验动物及瘤株 24只C57BL/6小鼠，雌雄各半，体质量（16±2）g，中国科学院实验动物中心提供，按清洁级标准饲养。小鼠Lewis肺癌细胞株和瘤块种鼠1只，由上海市肿瘤研究所姚明研究员惠赠。

1.1.2 实验药物肺积方由生黄芪30 g、北沙参12 g、麦冬12 g、天门冬12 g、茯苓15 g、石上柏30 g、石见穿30 g、鱼腥草15 g、七叶一枝花24 g和山慈菇30 g组成。浓煎成4 g/ml浓度，于4 ℃冰箱中贮存备用；顺铂由山东齐鲁制药厂生产，20 mg/支，批号为990711。

1.2 实验方法

1.2.1 造模及分组在无菌台上剥离瘤种的瘤块，在含有抗生素的生理盐水中将瘤块剪成1 mm×1 mm×1 mm的瘤块。取24只实验小鼠，将上述组织块采用20号套管针植入右侧腋后皮下。将小鼠随机分为模型组、顺铂组和肺积方组，每组8只，各组间小鼠体质量差异无统计学意义。接种后第2天开始灌胃干预治疗，共计20 d，治疗结束时处死取材。模型成功率100%，所有小鼠存活并纳入统计分析。

1.2.2 给药方法肺积方组：肺积方汤0.3 ml灌胃，1次/d，连续20 d。顺铂组：生理盐水配制成0.5 mg/L的顺铂溶液，根据人的常规用量换算［4］，每只0.52 ml/3 d，腹腔注射。模型组：生理盐水0.3 ml灌胃，1次/d，连续20 d。