纳米金溶胶制备及其尺寸分布的测量

论文题目：纳米金溶胶制备及其尺寸分布的测量

学生姓名：xx

指导教师：xx

摘要

纳米金具有很强的等离子吸收峰，其光学及电磁特性相对于其他金属纳米粒子更为突出，在DNA检测分析、生物探针、基团芯片、生物传感、药物载体等方面等多方面均有广泛的应用。实验室根据不同尺寸和形状的纳米金具有不同的光吸收能力这一特性，提出制备特定尺寸的纳米金用于血液注射以改善血液在近红外部分的吸光度的方案，从而为改进激光治疗葡萄酒色斑（Port Wine Stains，PWS）提供指导。

本文通过还原法制备出不同粒径的球状纳米金，主要考察了还原剂用量、保护剂用量、搅拌速度和搅拌强度对纳米金制备的影响。利用光谱仪和扫描电子显微镜对制得的纳米金的吸光特性和尺寸形貌进行表征。结果表明：采用柠檬酸钠还原法制备出15nm-24nm的球状纳米金颗粒，其中柠檬酸钠加入量为5mL时制备出的纳米金分散性较好，粒径分布较均匀；加入适量的聚乙烯吡咯烷酮（PVP）能有效地阻止纳米金团聚；在一定范围内的搅拌强度和搅拌时间对制备纳米金影响不大，但考虑到化学反应的需要和水蒸发过多对实验结果造成的不良影响，实验时搅拌强度以不产生漩涡、搅拌时间控制在15分钟左右为宜；采用柠檬酸钠-鞣酸还原法制备出3nm、9nm、16nm 左右的球状纳米金颗粒，随着鞣酸加入量的增加，制得的纳米金颗粒的尺寸减小；采用硼氢化钠还原法制备出的粒径约为24nm、28nm、33nm左右的球状纳米金颗粒，但其粒径分布不均匀，且形状相对不规则。

关键词：纳米金；还原法；柠檬酸钠；鞣酸；硼氢化钠

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Title: Preparation of gold nanoparticles and measuring the size distribution. Applicant: xx

Supervisor: xx

ABSTRACT

Golden nanoparticles have strong plasma absorption peak andbetter optical and electromagnetic properties than other metallic nanoparticles. Nowadaysthey are widely used in field of detection of DNA, drug delivery and manuscript of biological probes, group chip and biosensors. The golden nanoparticles with different sizes and shapes will have distinct light spectral absorption.Based on this characteristic, our lab proposed a method to prove the clinic effect of laser treatment of Port Wine Stains (PWS), a kind of congenital vascular malformation in dermis, byadding the golden nanoparticles to the blood to improve its light absorption.

In this paper, the spherical golden nanoparticles with various sizes (diameter) will be prepared by three chemical reduction methods experimentally andthe effects of the reducing agent, protective agent, stirring speed and stirring intensity on the nanoparticle preparation will be fully investigated.The spectral absorption and the morphology of golden nanoparticles are characterized by spectrometer and scanning electron microscopy, respectively. The experiment results show that: spherical golden nanoparticles with diameter of 15nm-24nm are produced by using sodium citrate reduction method.Good dispersion and uniform particle size distribution is observed when the amount of sodium citrate is 5ml. Aggregation of golden nanoparticles can be effectively prevented by adding PVP.The effects of stirring intensity and stirring time on the preparation of gold nanoparticles are small.Generally speaking, the stirring should not produce vortex in the sample and best stirring time is about 15 minutes, considering the chemical reaction and adverse effects of excessive water evaporation on the experimental results.Spherical golden nanoparticles with diameterof 3nm, 9nm and 16nm are produced by using sodium citrate-tannin acid reduction method.The sizes of gold nanoparticles willbe smaller if we increase of the amount of tannic acid.Spherical gold nanoparticles with diameter of 24nm, 28nm and 33nm will be prepared

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