适配体及其应用

Structure-Switching Signaling Aptamers
Βιβλιοθήκη Baidu
• we report on the preparation of several structure-switching reporters from two existing DNA aptamers.
Nutiu, R.; Li, Y. F. J. Am. Chem. Soc. 2003, 125(16), 4771-4778 .
展望
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简单化
可视化
高灵敏度
Optical Sensors Based on Hybrid Aptamer/Conjugated Polymer Complexes
• we describe here the use of a water soluble cationic polythiophene as a “polymeric stain” that can specifically transduce the binding of an aptamer to its target into a clear optical (colorimetric or fluorometric) signal.
高选择性
高灵敏度
目标分子 范围广
稳定性好
适配体传感器的应用范围
金属离子
药物分析
疾病检测
适配体传感器 应用
多重检测分析
蛋白质分子
靶向治疗
Designed Signaling Aptamers that Transduce Molecular Recognition to Changes in Fluorescence Intensity
• We have engineered aptamers that contain fluorescent reporters and that signal the presence of cognate ligands in solution.
S. Jhaveri, R. Kirby, R. Conrad, J. Am. Chem. Soc. 2000, 122(11), 2469-2473 .
Ho, H. A.; Leclerc, M. J. Am. Chem. Soc. 2004, 126(5), 1384-1387 .
Cationic Conjugated Polyelectrolytes-Triggered Conformational Change of Molecular Beacon Aptamer for Highly Sensitive and Selective Potassium Ion Detection
• Sensors for adenosine and cocaine were constructed, both of which can produce a color change within seconds at room temperature.
Liu, J. W.; Lu, Y. J. Am. Chem. Soc. 2006, 45(1), 90-94 .
适配体传感器及 其应用
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适配体传感器简介
适配体传感器原理及其特点
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适配体传感器相关文献
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展望
适配体传感器简介
适配体是通过指数富集系统进化技术SELEX 体外筛选得到的一类能够与靶分子特异结合 的寡核苷酸序列
指数富集系统进化技术SELEX
适配体传感器原理
适配体传感器特点
• We demonstrate highly sensitive and selective potassium ion detection against excess sodium ions in water,by modulating the interaction between the Gquadruplex-forming molecular beacon aptamer (MBA) and cationic conjugated polyelectrolyte (CPE). • The successful detection of 5′-adenosine triphosphate (ATP) with the MBA containing an ATP-specific aptamer sequence is also demonstrated using the same sensor scheme.
Structure-switching signaling aptamers: transducing molecular recognition into fluorescence signaling
R. Nutiu, Y. Li, Chem. Eur. J. 2004, 10, 1868-1876.
Kim, Boram.; Jung, In Hwan. J. Am. Chem. Soc. 2012, 134(6), 3133-3138 .
Fast Colorimetric Sensing of Adenosine and Cocaine Based on a General Sensor Design Involving Aptamers and Nanoparticles