荧光素概括
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700 nm
Far-Red
Alexa Fluor 700
702 nm
723 nm
Far-Red
Alexa Fluor 700–Allophycocyanin
650 nm
723 nm
Far-Red
Alexa Fluor 750
749 nm
775 nm
Far-Red
Alexa Fluor 750–Allophycocyanin
496, 546, 565 nm
668 nm
Red
Alexa Fluor 660
663 nm
690 nm
Red
Texas Red
596 nm
620 nm
Red
DyeMer 488/630
502 nm
630 nm
Red
Allophycocyanin
650 nm
660 nm
Red
PI
535 nm
617 nm
<400 nm
600 nm
Orange
Lissamine Rhodamine
570 nm
590 nm
Orange
MFP555
560 nm
585 nm
Orange
Qdot 585
532 nm
585 nm
Orange
Qdot 605
<600 nm
605 nm
Orange
ROX
575 nm
602 nm
Orange
578 nm
Yellow
B-Phycoerythrin
546, 565 nm
575 nm
Yellow
HNP/Fast Red TR
290 nm
562 nm
Yellow
5-TAMRA
546 nm
574 nm
Yellow
Atto 550
554 nm
576 nm
Yellow
CAL Fluor(R) Orange 560
Cy3B
558 nm
572 nm
Yellow
Alexa Fluor 532
531 nm
554 nm
Yellow
Alexa Fluor 546
556 nm
573 nm
Yellow
Alexa Fluor 555
555 nm
565 nm
Yellow
R-Phycoerythrin
480, 546, 565 nm
538 nm
559 nm
Yellow
Cyanine 3
550 nm
570 nm
Yellow
DyLight 547
547 nm
574 nm
Yellow
EviTag(TM) quantum dots - Birch Yellow (580 nm)
<400 nm
580 nm
Yellow
EviTag(TM) quantum dots - Hops Yellow (560 nm)
660 nm
Red
Sulforhodamine
585 nm
620 nm
Red
Texas Red
596 nm
620 nm
Red
Cyanine Q (Cyanine 5.18)
488 nm
670 nm
Red
NL637
637 nm
658 nm
Red
Allophycocyanin
620-650 nm
660-670 nm
Red
Cy5.5
673 nm
692 nm
Red
CypHer5E
644 nm
663 nm
Red
Alexa Fluor 594
590 nm
617 nm
Red
Alexa Fluor 594 FluoroNanogold
590 nm
617 nm
Red
Alexa Fluor 610
612 nm
628 nm
Red
618 nm
637 nm
Red
Cyanine 5
650 nm
670 nm
Red
DyLight 647
647 nm
673 nm
Red
DyeMer 488/630
488 nm
630 nm
Red
Dyomics 647
660 nm
673 nm
Red
Lightcycler Red- 640
625 nm
640 nm
578 nm
603 nm
Orange
ROX
575 nm
602 nm
Orange
DyeMer 488/605
502 nm
609 nm
Orange
DyeMer 488/615
502 nm
615 nm
Orange
CAL Fluor(R) Red 590
569 nm
591 nm
Orange
CAL Fluor(R) Red 610
Green
DTAF/FITC
495 nm
520 nm
Green
dUTP-Biotin and Avidin FITC
495 nm
520 nm
Green
dUTP-FITC
495 nm
520 nm
Green
EviTag(TM) quantum dots - Adirondack Green (520 nm)
5-FAM
494 nm
522 nm
Green
6-FAM
492 nm
517 nm
Green
BODIPY FL
505 nm
513 nm
Green
CMNB-caged Fluorescein
494 nm
518 nm
Green
Cyanine 2
492 nm
510 nm
Green
DTAF
495 nm
520 nm
488 nm
703 nm
Far-Red
R-Phycoerythrin-Cyanine 7 (PC7)
488 nm
805 nm
488 nm
660 nm
Red
Quasar(R) 670
647 nm
667 nm
Red
R-Phycocyanin (R-PC)
614 nm
647 nm
Red
R-Phycoerythrin-Cyanine 5 (Cy-Chrome, PC5, Tri-Color, Spectral Red)
488 nm
400 nm
420 nm
Blue
EviTag(TM) quantum dots - Lake Placid Blue (490 nm)
<400 nm
490 nm
Blue
Fluoroblue
350 nm
450 nm
Blue
Image-Blue
380 nm
465 nm
Blue
Marina Blue
365 nm
569 nm
610 nm
Orange
Cyanine 3.5
581 nm
596 nm
Orange
DyeMer 488/605
源自文库488 nm
605 nm
Orange
DyeMer 488/615
488 nm
615 nm
Orange
EviTag(TM) quantum dots - Fort Orange (600 nm)
IRDYE800
778 nm
795 nm
Far-Red
IRDYE800CW
774 nm
800 nm
Far-Red
NIRD700
682 nm
701 nm
Far-Red
Qdot 705
<700 nm
705 nm
Far-Red
Qdot 800
<600 nm
800 nm
Far-Red
R-Phycoerythrin-Cyanine 5.5
650 nm
775 nm
Far-Red
Allophycocyanin-Cyanine 7
633 nm
760 nm
Far-Red
Cyanine 5.5
678 nm
703 nm
Far-Red
Cyanine 7
710 nm
805 nm
Far-Red
IRDYE700DX
689 nm
700 nm
Far-Red
460 nm
Blue
Pacific Blue
410 nm
455 nm
Green
Cy2
491 nm
509 nm
Green
Alexa Fluor 430
434 nm
539 nm
Green
Alexa Fluor 488
495 nm
519 nm
Green
Alexa Fluor 488 FluoroNanogold
495 nm
520 nm
Green
Hi FITC
495 nm
520 nm
Green
JOE
520 nm
548 nm
Green
MFP488
501 nm
523 nm
Green
Oregon Green 488
496 nm
524 nm
Green
Oregon Green 514
511 nm
530 nm
Green
Red
CytoCy5S
628 nm
638 nm
Far-Red
Alexa Fluor 680
679 nm
702 nm
Far-Red
Alexa Fluor 680–Allophycocyanin
650 nm
702 nm
Far-Red
Alexa Fluor 680-R-phycoerythrin
555 nm
<400 nm
520 nm
Green
EviTag(TM) quantum dots - Catskill Green (540 nm)
<400 nm
540 nm
Green
FITC (Fluorescein)
495 nm
520 nm
Green
Fluorescein-5-EX, succinimidyl ester (FSE)
R-Phycoerythrin-Texas Red (PE-Texas Red, ECD)
480-565 nm
615 nm
Orange
Rhodamine Red-X
570 nm
590 nm
Red
TMR red
540 (520-560) nm
580 (570-620) nm
Red
Cy5
646 nm
664 nm
Qdot 525
488 nm
515 nm
Green
TET
521 nm
536 nm
Green
CAL Fluor(R) Gold 540
522 nm
544 nm
Green
NL493
493 nm
514 nm
Green
GFP
395/475 nm
509 nm
Yellow
Cy3
548 nm
562 nm
Yellow
激发光波长
发射光波长
Blue
Alexa Fluor 350
346 nm
442 nm
Blue
Alexa Fluor 405
402 nm
421 nm
Blue
Pacific Blue
410 nm
455 nm
Blue
DAPI
340 nm
488 nm
Blue
AMCA
350 nm
450 nm
Blue
Cascade Blue
荧光素根据其发射波长可分为：
<499 nm，蓝色荧光（Blue）500-549 nm，绿色荧光（Green）
550-584 nm，黄色荧光（Yellow）585-615 nm，橙色荧光（Orange）
616-700 nm，红色荧光（Red）>=700 nm，远红外荧光（Far-Red）
荧光类型
荧光分子
Yellow
TRITC (Rhodamine)
550 nm
570 nm
Yellow
VIC
538 nm
554 nm
Yellow
NL557
557 nm
574 nm
Yellow
Phycoerythrin
480-566 nm
575 nm
Orange
Cy3.5
581 nm
596 nm
Orange
Alexa Fluor 568
495 nm
519 nm
Green
Alexa Fluor 500
503 nm
525 nm
Green
Alexa Fluor 514
518 nm
540 nm
Green
FITC
490 nm
520 nm
Green
Fluorescein
494 nm
518 nm
Green
HNP
290 nm
509 nm
Green
Alexa Fluor 610–R-PE
496, 546, 565 nm
630 nm
Red
Alexa Fluor 633
632 nm
647 nm
Red
Alexa Fluor 635
633 nm
647 nm
Red
Alexa Fluor 647
650 nm
668 nm
Red
Alexa Fluor 647–R-phycoerythrin
Red
Allophycocyanin (APC)
650 nm
660 nm
Red
Allophycocyanin-Cyanine 5
633 nm
670 nm
Red
Allophycocyanin-Cyanine 5.5
633 nm
690 nm
Red
Atto 647
645 nm
669 nm
Red
CAL Fluor(R) Red 635
<400 nm
560 nm
Yellow
HEX
535 nm
556 nm
Yellow
NED
546 nm
575 nm
Yellow
Qdot 565
<500 nm
565 nm
Yellow
Quasar(R) 570
548 nm
566 nm
Yellow
R-Phycoerythrin (R-PE)
488 nm
575 nm
Red
PBXL-1
545 nm
666 nm
Red
PBXL-3
614 nm
662 nm
Red
PerCP (Peridinin Chlorophyll)
490 nm
675 nm
Red
PerCP-Cy5.5
488 nm
695 nm
Red
Qdot 655
<620 nm
655 nm
Red
Quantum Red
Far-Red
Alexa Fluor 700
702 nm
723 nm
Far-Red
Alexa Fluor 700–Allophycocyanin
650 nm
723 nm
Far-Red
Alexa Fluor 750
749 nm
775 nm
Far-Red
Alexa Fluor 750–Allophycocyanin
496, 546, 565 nm
668 nm
Red
Alexa Fluor 660
663 nm
690 nm
Red
Texas Red
596 nm
620 nm
Red
DyeMer 488/630
502 nm
630 nm
Red
Allophycocyanin
650 nm
660 nm
Red
PI
535 nm
617 nm
<400 nm
600 nm
Orange
Lissamine Rhodamine
570 nm
590 nm
Orange
MFP555
560 nm
585 nm
Orange
Qdot 585
532 nm
585 nm
Orange
Qdot 605
<600 nm
605 nm
Orange
ROX
575 nm
602 nm
Orange
578 nm
Yellow
B-Phycoerythrin
546, 565 nm
575 nm
Yellow
HNP/Fast Red TR
290 nm
562 nm
Yellow
5-TAMRA
546 nm
574 nm
Yellow
Atto 550
554 nm
576 nm
Yellow
CAL Fluor(R) Orange 560
Cy3B
558 nm
572 nm
Yellow
Alexa Fluor 532
531 nm
554 nm
Yellow
Alexa Fluor 546
556 nm
573 nm
Yellow
Alexa Fluor 555
555 nm
565 nm
Yellow
R-Phycoerythrin
480, 546, 565 nm
538 nm
559 nm
Yellow
Cyanine 3
550 nm
570 nm
Yellow
DyLight 547
547 nm
574 nm
Yellow
EviTag(TM) quantum dots - Birch Yellow (580 nm)
<400 nm
580 nm
Yellow
EviTag(TM) quantum dots - Hops Yellow (560 nm)
660 nm
Red
Sulforhodamine
585 nm
620 nm
Red
Texas Red
596 nm
620 nm
Red
Cyanine Q (Cyanine 5.18)
488 nm
670 nm
Red
NL637
637 nm
658 nm
Red
Allophycocyanin
620-650 nm
660-670 nm
Red
Cy5.5
673 nm
692 nm
Red
CypHer5E
644 nm
663 nm
Red
Alexa Fluor 594
590 nm
617 nm
Red
Alexa Fluor 594 FluoroNanogold
590 nm
617 nm
Red
Alexa Fluor 610
612 nm
628 nm
Red
618 nm
637 nm
Red
Cyanine 5
650 nm
670 nm
Red
DyLight 647
647 nm
673 nm
Red
DyeMer 488/630
488 nm
630 nm
Red
Dyomics 647
660 nm
673 nm
Red
Lightcycler Red- 640
625 nm
640 nm
578 nm
603 nm
Orange
ROX
575 nm
602 nm
Orange
DyeMer 488/605
502 nm
609 nm
Orange
DyeMer 488/615
502 nm
615 nm
Orange
CAL Fluor(R) Red 590
569 nm
591 nm
Orange
CAL Fluor(R) Red 610
Green
DTAF/FITC
495 nm
520 nm
Green
dUTP-Biotin and Avidin FITC
495 nm
520 nm
Green
dUTP-FITC
495 nm
520 nm
Green
EviTag(TM) quantum dots - Adirondack Green (520 nm)
5-FAM
494 nm
522 nm
Green
6-FAM
492 nm
517 nm
Green
BODIPY FL
505 nm
513 nm
Green
CMNB-caged Fluorescein
494 nm
518 nm
Green
Cyanine 2
492 nm
510 nm
Green
DTAF
495 nm
520 nm
488 nm
703 nm
Far-Red
R-Phycoerythrin-Cyanine 7 (PC7)
488 nm
805 nm
488 nm
660 nm
Red
Quasar(R) 670
647 nm
667 nm
Red
R-Phycocyanin (R-PC)
614 nm
647 nm
Red
R-Phycoerythrin-Cyanine 5 (Cy-Chrome, PC5, Tri-Color, Spectral Red)
488 nm
400 nm
420 nm
Blue
EviTag(TM) quantum dots - Lake Placid Blue (490 nm)
<400 nm
490 nm
Blue
Fluoroblue
350 nm
450 nm
Blue
Image-Blue
380 nm
465 nm
Blue
Marina Blue
365 nm
569 nm
610 nm
Orange
Cyanine 3.5
581 nm
596 nm
Orange
DyeMer 488/605
源自文库488 nm
605 nm
Orange
DyeMer 488/615
488 nm
615 nm
Orange
EviTag(TM) quantum dots - Fort Orange (600 nm)
IRDYE800
778 nm
795 nm
Far-Red
IRDYE800CW
774 nm
800 nm
Far-Red
NIRD700
682 nm
701 nm
Far-Red
Qdot 705
<700 nm
705 nm
Far-Red
Qdot 800
<600 nm
800 nm
Far-Red
R-Phycoerythrin-Cyanine 5.5
650 nm
775 nm
Far-Red
Allophycocyanin-Cyanine 7
633 nm
760 nm
Far-Red
Cyanine 5.5
678 nm
703 nm
Far-Red
Cyanine 7
710 nm
805 nm
Far-Red
IRDYE700DX
689 nm
700 nm
Far-Red
460 nm
Blue
Pacific Blue
410 nm
455 nm
Green
Cy2
491 nm
509 nm
Green
Alexa Fluor 430
434 nm
539 nm
Green
Alexa Fluor 488
495 nm
519 nm
Green
Alexa Fluor 488 FluoroNanogold
495 nm
520 nm
Green
Hi FITC
495 nm
520 nm
Green
JOE
520 nm
548 nm
Green
MFP488
501 nm
523 nm
Green
Oregon Green 488
496 nm
524 nm
Green
Oregon Green 514
511 nm
530 nm
Green
Red
CytoCy5S
628 nm
638 nm
Far-Red
Alexa Fluor 680
679 nm
702 nm
Far-Red
Alexa Fluor 680–Allophycocyanin
650 nm
702 nm
Far-Red
Alexa Fluor 680-R-phycoerythrin
555 nm
<400 nm
520 nm
Green
EviTag(TM) quantum dots - Catskill Green (540 nm)
<400 nm
540 nm
Green
FITC (Fluorescein)
495 nm
520 nm
Green
Fluorescein-5-EX, succinimidyl ester (FSE)
R-Phycoerythrin-Texas Red (PE-Texas Red, ECD)
480-565 nm
615 nm
Orange
Rhodamine Red-X
570 nm
590 nm
Red
TMR red
540 (520-560) nm
580 (570-620) nm
Red
Cy5
646 nm
664 nm
Qdot 525
488 nm
515 nm
Green
TET
521 nm
536 nm
Green
CAL Fluor(R) Gold 540
522 nm
544 nm
Green
NL493
493 nm
514 nm
Green
GFP
395/475 nm
509 nm
Yellow
Cy3
548 nm
562 nm
Yellow
激发光波长
发射光波长
Blue
Alexa Fluor 350
346 nm
442 nm
Blue
Alexa Fluor 405
402 nm
421 nm
Blue
Pacific Blue
410 nm
455 nm
Blue
DAPI
340 nm
488 nm
Blue
AMCA
350 nm
450 nm
Blue
Cascade Blue
荧光素根据其发射波长可分为：
<499 nm，蓝色荧光（Blue）500-549 nm，绿色荧光（Green）
550-584 nm，黄色荧光（Yellow）585-615 nm，橙色荧光（Orange）
616-700 nm，红色荧光（Red）>=700 nm，远红外荧光（Far-Red）
荧光类型
荧光分子
Yellow
TRITC (Rhodamine)
550 nm
570 nm
Yellow
VIC
538 nm
554 nm
Yellow
NL557
557 nm
574 nm
Yellow
Phycoerythrin
480-566 nm
575 nm
Orange
Cy3.5
581 nm
596 nm
Orange
Alexa Fluor 568
495 nm
519 nm
Green
Alexa Fluor 500
503 nm
525 nm
Green
Alexa Fluor 514
518 nm
540 nm
Green
FITC
490 nm
520 nm
Green
Fluorescein
494 nm
518 nm
Green
HNP
290 nm
509 nm
Green
Alexa Fluor 610–R-PE
496, 546, 565 nm
630 nm
Red
Alexa Fluor 633
632 nm
647 nm
Red
Alexa Fluor 635
633 nm
647 nm
Red
Alexa Fluor 647
650 nm
668 nm
Red
Alexa Fluor 647–R-phycoerythrin
Red
Allophycocyanin (APC)
650 nm
660 nm
Red
Allophycocyanin-Cyanine 5
633 nm
670 nm
Red
Allophycocyanin-Cyanine 5.5
633 nm
690 nm
Red
Atto 647
645 nm
669 nm
Red
CAL Fluor(R) Red 635
<400 nm
560 nm
Yellow
HEX
535 nm
556 nm
Yellow
NED
546 nm
575 nm
Yellow
Qdot 565
<500 nm
565 nm
Yellow
Quasar(R) 570
548 nm
566 nm
Yellow
R-Phycoerythrin (R-PE)
488 nm
575 nm
Red
PBXL-1
545 nm
666 nm
Red
PBXL-3
614 nm
662 nm
Red
PerCP (Peridinin Chlorophyll)
490 nm
675 nm
Red
PerCP-Cy5.5
488 nm
695 nm
Red
Qdot 655
<620 nm
655 nm
Red
Quantum Red