微流控分析芯片用于凝血检测的实验研究

河北工业大学

硕士学位论文

微流控分析芯片用于凝血检测的实验研究

姓名：孟庆宜

申请学位级别：硕士

专业：测试计量技术及仪器

指导教师：张思祥

20071201

河北工业大学硕士学位论文

微流控分析芯片用于凝血检测的实验研究

摘要

随着科学技术的发展和基础医学研究的进步，人们对止血与血栓的发生发展认识越来越深刻，其检测手段也越来越先进。与此同时，分析仪器也日益向着微型化、集成化与便携化的趋势发展。其中，微分析系统是这一发展时期的典型代表，随着微流控芯片技术的发展，特别是检测灵敏度的日臻提高，用微流控芯片对微量物质的分析与检测日益受到重视。本课题研究的目的就是采用微流控芯片作为检测容器，在凝血检测中最大限度地把加试剂、反应、检测等分析功能集成为一体。

本课题在分析、总结了传统的检测原理及方法的基础上，设计了利用微流控芯片进行凝血四项检测的基本方法。首先我们利用步进电机设计了离心力驱动微流控芯片结构，该结构可在芯片旋转过程中完成血浆与试剂的混合、反应等操作。同时，运用能发射红光和蓝光的双光束二极管为恒定光源，光束透过芯片的检测区，在其另一面有硅光电池接收光信号。在与凝结剂均匀混合后，血浆由于发生一定的理化反应而凝结，光信号也会随之发生改变。硅光电池将光信号的变化转换成电信号的变化，经采集可显示整个凝结过程图像，经数据处理后计算，可得到需要的信息。

本课题根据检测原理重点对检测装置的机械部分进行了设计和改善，选择了微流控芯片来替代传统的玻璃试管作为检测容器，以改进用玻璃试管检测时所存在的问题，提高精度和可靠性；同时，对检测系统的硬件电路部分和基于LabVIEW 的检测系统操作软件进行了设计和完善，以提高系统的准确性，可靠性和稳定性；并且在研究和改进过程中完成了大量有效的实验。

采用微流控芯片作为检测容器与传统玻璃试管相比，试剂混合均匀，能去除透镜效应，且实验一致性好。该检测系统的研究成功将进一步促进我国医学检测技术的发展，同时，将加快微流控芯片的产业化，为分析仪器提供新的经济增长点。

关键词: 微流控芯片，凝血检测，虚拟仪器，离心力驱动

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微流控分析芯片用于凝血检测的实验研究

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RESAERCH OF CRUOR MEASURING SYSTEM

USING MICROFLUIDIC CHIP

ABSTRACT

With the development of science and the progress of medical research, people’s

understanding through thrombus and hemostasis is more and more profound, the request of examine method is also more and more advanced. At the same time, analytic instrument has had the current characteristics of micromation, integration and conveniency day after day. μTAS is the representation in this period. With the development of microfluidic technology, especially the advancement in detection, it is more and more important to analyse and detect micro-substance using microfluidic chips. Our research is to use microfluidic chip as detecting container, and the function of injecting the reagent, reacting and detecting are to be done all together.

On the basis of analyzing and summarizing the traditional examination principle and method, our work studies the basic method of cruor testing using microfluidic chip. Firstly, we design a frame called centrifugal force running microfluidic chip, which can finish the mixture and reaction of the plasm and reagent during the chip’s circumrotation. Then we take the red light or blue light as the constant photosource. The light beam goes through the detecting area, and on the other side of the chip there is a silicon photronic generator which will receive the luminous intensity signals. Because of the chemical reaction, the plasma congeals, so the luminous intensity changes. The silicon photoelectric generator transforms the luminous intensity signals to the electrical signals. After acquiring the electrical signal, the entire process of congeals may be demonstrated. And after the data processing and calculation, the requested information is acquired.

Our work mainly designed and improved the machine part of the system. We chose microfluidic chip to substitute the traditional cuvette as testing container, so as to correct the objection of cuvette, as well as improve the precision and reliability of the system. Meanwhile, we improved the hardware of the system and designed the software based on the LabVIEW platform servers, to improve the accuracy, reliability and stability of the system. During the designing and improving process, we have also done many effective experiments.

Compared with the traditional cuvette system, using microfluidic chip as testing container can wipe off lens effect, the plasm and the reagent mixed uniformly, and the experiments have good consistency. The success of this system will promote the medicine examination technology of our country. It can also quicken the microfluidic chip industrialization and provide analytic instrument new economic increase.

KEY WORDS: microfluidic chip, cruor testing, LabVIEW, centrifugal force running