高密度脂蛋白抗动脉粥样硬化机制—SRBI,CD36,PPARgamma的作用课件
合集下载
- 1、下载文档前请自行甄别文档内容的完整性，平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益，请联系客服反馈,我们会尽快为您处理(人工客服工作时间：9:00-18:30)。
Figure 1: The mean body weights of the two groups throughout the study
5
HFHC CONTROL
动脉粥样硬化小型猪血脂水平的改变
450 400 350 300 250 200 150 100 50 0 1 2 3 4 5
80
P la sm a HD L- C( mg /d l)
7
As小型猪主动脉HE染色(×20)
对照组
实验组
8
A
B
Figure High fat/high cholesterol diet-induced atherosclerotic plaque was stained with hematoxylin eosin in mini swine. Magnification is ×40 A: sections (5 µm thick) were taken at the abdominal aorta in control fed pigs; B: sections (5 µm thick) were taken at the abdominal aorta in HFHC-fed pigs.
1.5 *
Relative Density
*
1.2 0.9 0.6 0.3 0 1
*
500bp
GAPDH 596bp CD36 246bp
2
3
4
5
6
M
1
2
3
4
5
6
1: 对照组肝脏; 3: 对照组肾脏; 5: 对照组主动脉； 2: 实验组肝脏; 4: 实验组肾脏; 6: 实验组主动脉. * P<0.05,与对照组比较
12
A
B
Figure 8: Expression of SR-BI protein in mini swine(×40) (n=5) A: Liver in control group mini swine; B: Liver in mini swine fed with HFHC;
13
As小型猪组织CD36 mRNA的表达变化
Relative Density
动脉粥样硬化小型猪 SR-BI 蛋白表达的变化
1 2 3 4
3
Relative Density
2.5 2 1.5 1 0.5 0 1 2 3 4
Figure 7: Expression of SR-BI protein in mini swine fed with HFHC.(n=5) 1: Liver in control group mini swine; 2: Liver in mini swine fed with HFHC; 3: Aorta in control group mini swine; 4: Aorta in mini swine fed with HFHC. *: P<0.05, compared with control group.
9
动脉粥样硬化小型猪动脉及肝脏HE染色
A
B
Figure 5: High fat/high cholesterol diet-induced fatty liver was stained with hematoxylin eosin in mini swine. Magnification is ×40 A: sections (5 µm thick) were taken at the liver in control fed pigs; B: sections (5 µm thick) were taken at the live in HFHC-fed pigs.
90 80
Plasma TG(mg/dl)
Plasma TC(mg/dl)
70 60 50 40 30 20 10 0 CONTROL HFHC
70 60 50 40 30 20 10 0 CONTROL HFHC
CONTROL HFHC
6
7
1
2
3
4
5
6
7
1
2
3
4
5
6
7
Time(month)
Time(month)
Time(month)
Figure 2: Plasma level of total cholesterol(a), HDL-cholesterol(b) and triglyceride(c) in mini swine
6
动脉粥样硬化小型猪冠状动脉苏丹Ⅳ染色
A
B
Figure 3: High fat/high cholesterol diet-induced atherosclerosis in mini swine. A: photograph of pinned-out coronary artery in control fed pigs; B: photograph of pinned-out coronary artery in HFHC-fed pigs.
10
SR-BI mRNA在动脉粥样硬化小型猪组织表达的变化
M 1 2 3 4
GAPDH SR-BI
*
1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 1 2 3 4
Figure 6: Expression of SR-BI mRNA in mini swine fed with HFHC 1: Liver in control group mini swine; 2: Liver in mini swine fed with HFHC; 3: Aorta in control group mini swine; 4: Aorta in mini swine fed with HFHC; M：Markers；*: P<0.05, compared with control group. （n=5) 11
高密度脂蛋白抗动脉粥样硬 化机制—SR-BI, CD36, PPARgamma的作用
1
泡沫细胞形成是动脉粥样硬化发病的核心
2
SR-BI
3
第一部分
动脉粥样硬化小型猪SR-BI、 CD36和PPARγ表达的变化
4
动脉粥样硬化小型猪体重的变化
Body weight(kg)
40 30 20 10 0 0 2 4 6 8 10 12 Time(month)
5
HFHC CONTROL
动脉粥样硬化小型猪血脂水平的改变
450 400 350 300 250 200 150 100 50 0 1 2 3 4 5
80
P la sm a HD L- C( mg /d l)
7
As小型猪主动脉HE染色(×20)
对照组
实验组
8
A
B
Figure High fat/high cholesterol diet-induced atherosclerotic plaque was stained with hematoxylin eosin in mini swine. Magnification is ×40 A: sections (5 µm thick) were taken at the abdominal aorta in control fed pigs; B: sections (5 µm thick) were taken at the abdominal aorta in HFHC-fed pigs.
1.5 *
Relative Density
*
1.2 0.9 0.6 0.3 0 1
*
500bp
GAPDH 596bp CD36 246bp
2
3
4
5
6
M
1
2
3
4
5
6
1: 对照组肝脏; 3: 对照组肾脏; 5: 对照组主动脉； 2: 实验组肝脏; 4: 实验组肾脏; 6: 实验组主动脉. * P<0.05,与对照组比较
12
A
B
Figure 8: Expression of SR-BI protein in mini swine(×40) (n=5) A: Liver in control group mini swine; B: Liver in mini swine fed with HFHC;
13
As小型猪组织CD36 mRNA的表达变化
Relative Density
动脉粥样硬化小型猪 SR-BI 蛋白表达的变化
1 2 3 4
3
Relative Density
2.5 2 1.5 1 0.5 0 1 2 3 4
Figure 7: Expression of SR-BI protein in mini swine fed with HFHC.(n=5) 1: Liver in control group mini swine; 2: Liver in mini swine fed with HFHC; 3: Aorta in control group mini swine; 4: Aorta in mini swine fed with HFHC. *: P<0.05, compared with control group.
9
动脉粥样硬化小型猪动脉及肝脏HE染色
A
B
Figure 5: High fat/high cholesterol diet-induced fatty liver was stained with hematoxylin eosin in mini swine. Magnification is ×40 A: sections (5 µm thick) were taken at the liver in control fed pigs; B: sections (5 µm thick) were taken at the live in HFHC-fed pigs.
90 80
Plasma TG(mg/dl)
Plasma TC(mg/dl)
70 60 50 40 30 20 10 0 CONTROL HFHC
70 60 50 40 30 20 10 0 CONTROL HFHC
CONTROL HFHC
6
7
1
2
3
4
5
6
7
1
2
3
4
5
6
7
Time(month)
Time(month)
Time(month)
Figure 2: Plasma level of total cholesterol(a), HDL-cholesterol(b) and triglyceride(c) in mini swine
6
动脉粥样硬化小型猪冠状动脉苏丹Ⅳ染色
A
B
Figure 3: High fat/high cholesterol diet-induced atherosclerosis in mini swine. A: photograph of pinned-out coronary artery in control fed pigs; B: photograph of pinned-out coronary artery in HFHC-fed pigs.
10
SR-BI mRNA在动脉粥样硬化小型猪组织表达的变化
M 1 2 3 4
GAPDH SR-BI
*
1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 1 2 3 4
Figure 6: Expression of SR-BI mRNA in mini swine fed with HFHC 1: Liver in control group mini swine; 2: Liver in mini swine fed with HFHC; 3: Aorta in control group mini swine; 4: Aorta in mini swine fed with HFHC; M：Markers；*: P<0.05, compared with control group. （n=5) 11
高密度脂蛋白抗动脉粥样硬 化机制—SR-BI, CD36, PPARgamma的作用
1
泡沫细胞形成是动脉粥样硬化发病的核心
2
SR-BI
3
第一部分
动脉粥样硬化小型猪SR-BI、 CD36和PPARγ表达的变化
4
动脉粥样硬化小型猪体重的变化
Body weight(kg)
40 30 20 10 0 0 2 4 6 8 10 12 Time(month)