臭氧灭活水中铜绿微囊藻影响因素研究

中国环境科学 2012,32(4)：653~658 China Environmental Science 臭氧灭活水中铜绿微囊藻影响因素研究

汪小雄*,姜成春,朱佳,谢炜平(深圳职业技术学院建筑与环境工程学院,广东深圳 518055)

摘要：为研究臭氧在水体中杀灭铜绿微囊藻的效果,利用中性红染色法探讨了不同因素(臭氧投量、作用时间、pH值、温度、浑浊度、初始藻细胞密度等)对臭氧灭活铜绿微囊藻效果的影响.结果表明,随着臭氧投量和作用时间的延长,藻灭活率明显增加.当浑浊度0.5~20NTU,温度5~35,℃ pH值6.0~9.0,同时浊度越低,灭活效果越好;随温度上升,臭氧灭活铜绿微囊藻能力减弱;碱性较酸性条件下臭氧杀灭铜绿微囊藻的能力更强.藻样初始浓度对杀藻效果影响较大,细胞密度增大,杀藻效果急剧下降.当初始藻细胞密度为1.0×107cells/L,臭氧投量为2.0mg/L,作用时间40min以上时,在饮用水消毒的浊度、温度、pH值范围内,铜绿微囊藻的灭活率在99.0%以上,繁殖能力降低到0.

关键词：铜绿微囊藻；臭氧；灭活；中性红

中图分类号：X172 文献标识码：A 文章编号：1000-6923(2012)04-0653-06

Effect of various factors on ozone inactivating Microcystis aeruginosa in water. WANG Xiao-xiong*, JIANG Cheng-chun, ZHU Jia, XIE Wei-ping (School of Civil and Environmental Engineering, Shenzhen Polytechnic, Shenzhen 518055, China). Environmental Science, 2012,32(4)：653~658

Abstract：In order to study the effect of O3 inactivating Microsystis aeruginosa in water,different factors (the ozone dose, time, pH, temperature, turbidity and cell density) which might influence the inactivation were investigated by using neutral red staining assays. The results indicated that the inactivated rate of O3 affecting Microsystis aeruginosa increased as the ozone dose and time prolonging obviously. When the turbidity was 0.5 to 20.0NTU,temperature was 5 to 35℃, pH was 6.0 to 9.0, the turbidity was lower, the higher inactivating ratio could be received. With the increasing of temperature, the inactivating effect was decreased. The ability of O3 inactivating Microsystis aeruginosa was stronger under acidic condition than it was in alkali circumstance. If cell density increased, the inactivation efficiency decreased significantly. The initial algal concentration also influenced the treatment efficiency. If cell density increased the inactivation efficiency decreased significantly. If the initial algal concentration was 1.0×107 cells/L, the ozone dose 2.0 mg/L and the time was more than 40 min, the ratio of inactivation could exceed 99.0% and the capability of propagator was zero during disinfecting drinking water.

Key words：Microsystis aeruginosa；O3；inactivating；neutral red

世界上淡水湖泊藻类水华发生的频率与严重程度都呈现增长的趋势,其中蓝藻是引起藻类水华污染的主要藻类[1].水体中藻类的大量繁殖不仅使水体水质状况恶化,而且对饮用水的处理和安全也带来影响,威胁饮用水的安全性:某些藻类释放藻毒素引起人畜患病甚至死亡[2-3],藻细胞及它的胞外分泌物在氯化过程中产生三卤甲烷、卤乙酸、卤乙氰等物质[4-7];具有鞭毛的藻细胞易穿透絮凝体,从而破坏絮凝过程,导致出水存在藻细胞;藻细胞在滤床中的生长又会导致滤床产生堵塞.以上问题的存在,说明饮用水处理中不能忽视藻类的影响.一直以来,国内水处理预氧化一直以预氯化为主,预氯化对藻类等微生物灭活、助凝提高藻类及水体颗粒物去除虽有较好的效果[8-9],但氯氧化或消毒产生的消毒副产物引起国内外饮用水处理界的高度重视.臭氧作为氯预氧化的良好替代品,臭氧氧化和强化混凝被认为是控制消毒副产物的最佳可行性技术[9-12].

以往的各种研究,通常使用藻细胞密度、光密度、叶绿素含量来衡量除藻率,实际工作中这

收稿日期：2011-07-28

基金项目：国家自然科学基金项目(50978169);深圳市科技计划项目(2109K3080011)

* 责任作者, 助理研究员, wangxiaoxiong20@