蛋白质翻译后修饰
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Protein Modification 蛋白质修饰简介
©2014 Waters Corporation
1
蛋白质修饰存在的类型 Phosphorylation (磷酸化) Acetylation (乙酰化) Methylation (甲基化) Ubiquitination (泛素化) SUMOylation (small ubiquitin-related modifier) Glycosylation (糖基化) Myristylation (十四烷基化) and Farnesylation (法尼基化)
©2014 Waters Corporation
5
糖基化
N-linked glycosylation (Asn) 发生的序列位置为NXS/T，其中X可以为任何氨基酸，但P除外。
O-linked glycosylation (Ser, Thr)
©2014 Waters Corporation
6
酰胺化
Subcellular localization
modification site may be a targeting signal modification may be a membrane anchor
Degradation ……
identify the protein for degradation
©2014 Waters CorporSTY时，发生磷酸化修饰
©2014 Waters Corporation
4
甲基化
常见修饰，发生在精氨酸或赖氨酸 Arginine can be methylated once or twice by Peptidylarginine methyltransferases (PRMTs) Lysine can be methylated once, twice or three times by lysine methyltransferases .
甘氨酸羟基化，然后脱乙醛酸，并产生新的酰胺化羧基端 N-端谷氨酸残基的氨基与羧基脱水形成焦谷氨酸
©2014 Waters Corporation
7
©2014 Waters Corporation
2
蛋白质修饰存在的意义
Regulation of activity
turn activity on turn activity off generate a different function
Protein-protein interaction
modification site may be a binding interface
©2014 Waters Corporation
1
蛋白质修饰存在的类型 Phosphorylation (磷酸化) Acetylation (乙酰化) Methylation (甲基化) Ubiquitination (泛素化) SUMOylation (small ubiquitin-related modifier) Glycosylation (糖基化) Myristylation (十四烷基化) and Farnesylation (法尼基化)
©2014 Waters Corporation
5
糖基化
N-linked glycosylation (Asn) 发生的序列位置为NXS/T，其中X可以为任何氨基酸，但P除外。
O-linked glycosylation (Ser, Thr)
©2014 Waters Corporation
6
酰胺化
Subcellular localization
modification site may be a targeting signal modification may be a membrane anchor
Degradation ……
identify the protein for degradation
©2014 Waters CorporSTY时，发生磷酸化修饰
©2014 Waters Corporation
4
甲基化
常见修饰，发生在精氨酸或赖氨酸 Arginine can be methylated once or twice by Peptidylarginine methyltransferases (PRMTs) Lysine can be methylated once, twice or three times by lysine methyltransferases .
甘氨酸羟基化，然后脱乙醛酸，并产生新的酰胺化羧基端 N-端谷氨酸残基的氨基与羧基脱水形成焦谷氨酸
©2014 Waters Corporation
7
©2014 Waters Corporation
2
蛋白质修饰存在的意义
Regulation of activity
turn activity on turn activity off generate a different function
Protein-protein interaction
modification site may be a binding interface