病毒的复制(2).ppt

• Nucleic acid may be made in nucleus
•
（核） or cytoplasm（胞浆）
Protein synthesis is always in the cytoplasm
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Synthesis
the production of early mRNA & early proteins
Release
1 Lysis裂解 2 Budding through plasma membrane
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Semliki Forest Virus塞姆利基森林病毒 Budding
Schaechter et al, Mechanisms of Microbial Disease, 3rd ed, 1998
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Penetration
NON-ENVELOPED VIRUSES
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Penetration Naked virus
entry directly across plasma membrane
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Penetration Naked virus
entry directly across plasma membrane
Viral multiplication 病毒增殖
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Basic Steps of Viral Baidu Nhomakorabeaeplication Cycle
病毒的复制周期
• Adsorption吸附 • Penetration穿入 • Uncoating脱壳 • Biosynthesis生物合成 • (eclipse phase隐蔽期) • Assembly装配& Release释放
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HIV Budding & Maturation
Hsiung, GD et al., Diagnostic Virology 1994 p204 (D. Medina)
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uninfected
HIV infected
HIV infected (at higher magnifcation)
Hockley et al. J Gen Virol 69:2455-2469
ribosomes
picornaviruse to serve as a messenger RNA to serve as a template for the synthesis of a complementary (-) strand RNA -- make more (+) RNA strands
the replication of genome
the production of late mRNA & late proteins
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1. ds DNA virus
Cytoplasm`
nsp
nucleus
sp
Herpesviruse 疱疹病毒
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2. (+) Single-stranded RNA viruses
• Once uncoating occurs, enter eclipse phase
• Eclipse phase lasts until first new virus particle formed
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Synthesis合成
Viral Nucleic Acid & Protein
• Many strategies（多种策略）
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UNCOATING
• Need to make genome available
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UNCOATING
• Need to make genome available • Once uncoating occurs, enter eclipse
phase
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UNCOATING
• Need to make genome available
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ADSORPTION
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ADSORPTION
• Temperature independent • Requires viral specific binding site • Cellular receptors细胞的受体
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Significance
The specific attachment
• Fusion with cell membrane • Entry via endosomes, fusion with acidic endosome membrane
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Penetration Enveloped Viruses
from Schaechter et al, Mechanisms of Microbial Disease, 3rd ed, 1998
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Smallpox Virus天花病毒 Assembly & Maturation
F. A. Murphy, School of Veterinary Medicine, University of California, Davis.
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http://www.vetnet.ucdavis.edu/fam_ graphics/ downlo ad. html
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3. (-) Single-stranded RNA viruses
Paramyxovirus 副粘病毒
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4.Retroviruse
nucleus
(proviru(ps，ro前vi病ru毒s))
HIV,HTLV-l 30
Assembly & Maturation
Nucleus Cytoplasm At membrane
determines the host range（属主
范围） of the virus:
:
• Species（种特异性）
• organ specificity（器官特异性）
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Penetration Enveloped Viruses
• Fusion with cell membrane • Entry via endosome（经内吞体）
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Penetration
herpesviruses, paramyxoviruses, HIV
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Penetration Enveloped Viruses
• Fusion with cell membrane • Entry via endosome
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Penetration Enveloped Viruses