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Ex1 and Ex2
Increase minerals (N, P, K and Mg), and both Chl inhibiting spore biosynthesis and germination the photosynthetic activity ， accumulation of metabolites. 8h S+T > Ex1 +Ex2
Influence of Plant Extracts and Microbioagents on Physiological Traits of Faba Bean Infected with Botrytis fabae(蚕豆葡萄孢盘菌)
CONTENTS
1 2 ABSTRACT BACKGROUND
16h Ex1 +Ex2 > S+T The efficiency test+Ex2 biocontrol agents seemed to be in the 4 days S+T of > Ex1 > T > Ex2 > Ex1 = S
order: T+S > Ex1 +Ex2 > T > S > Ex2 > Ex1.
3.2 Preparation of B. fabae Spore Suspension
Isolated from the infected faba bean leaves—Identified—PDA medium—the spores（4 x 104 CFU m-1）
吴冰月
3 MATERIALS AND METHODS
Greenhouse experiments Laboratory experiments Reduce the activities of peroxidase(过氧化物酶), catalase
（过氧化氢酶） pectinase （果胶酶） in the infected controlling the and growth of B. fabae plants.
2
BACKGROUND
Vicia faba
Botrytis fabae
赤斑病
Antifungal compounds
Plant extracts Streptomycetes(S)
Trichoderma(T)
赤斑病
2
BACKGROUND
(1) Studying the role of selected plant extracts (added singly or in combination), S. exfloliatus and T.harzianum (added singly or in combination), to reduce the detrimental effects of B fabae on faba bean plants (2) Finding an explanation for the above role based on test attributes (3) Evaluating the enhancement of yield of plants (4) Finding a recommendation for controlling the fungal disease
3 MATERIALS AND METHODS 4 5 RESULTS
DISCUSSION
1
ABSTRACT
Eucalyptus citriodora 柠檬桉( Ex1); Ipomoea carnea 树牵牛( Ex2) ; Cuminum cyminum 孜然芹( Ex3); Allium sativum 大蒜 ( Ex4); Hyoscyamus muticus纯天仙子 ( Ex5) Streptomyces exfloliatus 脱叶链霉菌(S) Trichoderma harzianum 哈茨木酶 (T)
3 MATERIALS AND METHODS
Grains of V. faba
3.1Plant Materials
Test biocontrol agents included plant leaf extracts, S. exfloliatus (S) and T. harzianum (T)
吴冰月
3 MATERIALS AND METHODS 3.1 Plant Materials 3.2 Preparation of B. fabae Spore Suspension 3.3 Preparation of Plant Extracts 3.4 Preparation of Microbioagent Suspensions 3.5 Laboratory Experiments 3.6 Greenhouse Experiment 3.7 Growth Conditions 3.8 Physiological Measurements 3.9 Enzyme Assay 3.10 Mineral Concentration 3.11 Chlorophyll (Chl) Concentraion 3.12 Photosynthetic (Hill-Reaction) Activity 3.13 Metabolite Concentration 吴冰月 3.14 Growth Criteria and Seed Yield 3.15 Statistical Analysis
3.3 Preparation
of Plantቤተ መጻሕፍቲ ባይዱExtracts
Grinding leaves（100g-200ml dh2o)—Filtrate—Centrifuged （3000rpm,15min) — lyophilized—re-extracted （methanol）—collect the organic layer—evaporated— dry matter was dissolved （10ml dh2o ) —centrifuged （ 3000rpm,15min ）
Increase minerals (N, P, K and Mg), and both Chl inhibiting spore biosynthesis and germination the photosynthetic activity ， accumulation of metabolites. 8h S+T > Ex1 +Ex2
Influence of Plant Extracts and Microbioagents on Physiological Traits of Faba Bean Infected with Botrytis fabae(蚕豆葡萄孢盘菌)
CONTENTS
1 2 ABSTRACT BACKGROUND
16h Ex1 +Ex2 > S+T The efficiency test+Ex2 biocontrol agents seemed to be in the 4 days S+T of > Ex1 > T > Ex2 > Ex1 = S
order: T+S > Ex1 +Ex2 > T > S > Ex2 > Ex1.
3.2 Preparation of B. fabae Spore Suspension
Isolated from the infected faba bean leaves—Identified—PDA medium—the spores（4 x 104 CFU m-1）
吴冰月
3 MATERIALS AND METHODS
Greenhouse experiments Laboratory experiments Reduce the activities of peroxidase(过氧化物酶), catalase
（过氧化氢酶） pectinase （果胶酶） in the infected controlling the and growth of B. fabae plants.
2
BACKGROUND
Vicia faba
Botrytis fabae
赤斑病
Antifungal compounds
Plant extracts Streptomycetes(S)
Trichoderma(T)
赤斑病
2
BACKGROUND
(1) Studying the role of selected plant extracts (added singly or in combination), S. exfloliatus and T.harzianum (added singly or in combination), to reduce the detrimental effects of B fabae on faba bean plants (2) Finding an explanation for the above role based on test attributes (3) Evaluating the enhancement of yield of plants (4) Finding a recommendation for controlling the fungal disease
3 MATERIALS AND METHODS 4 5 RESULTS
DISCUSSION
1
ABSTRACT
Eucalyptus citriodora 柠檬桉( Ex1); Ipomoea carnea 树牵牛( Ex2) ; Cuminum cyminum 孜然芹( Ex3); Allium sativum 大蒜 ( Ex4); Hyoscyamus muticus纯天仙子 ( Ex5) Streptomyces exfloliatus 脱叶链霉菌(S) Trichoderma harzianum 哈茨木酶 (T)
3 MATERIALS AND METHODS
Grains of V. faba
3.1Plant Materials
Test biocontrol agents included plant leaf extracts, S. exfloliatus (S) and T. harzianum (T)
吴冰月
3 MATERIALS AND METHODS 3.1 Plant Materials 3.2 Preparation of B. fabae Spore Suspension 3.3 Preparation of Plant Extracts 3.4 Preparation of Microbioagent Suspensions 3.5 Laboratory Experiments 3.6 Greenhouse Experiment 3.7 Growth Conditions 3.8 Physiological Measurements 3.9 Enzyme Assay 3.10 Mineral Concentration 3.11 Chlorophyll (Chl) Concentraion 3.12 Photosynthetic (Hill-Reaction) Activity 3.13 Metabolite Concentration 吴冰月 3.14 Growth Criteria and Seed Yield 3.15 Statistical Analysis
3.3 Preparation
of Plantቤተ መጻሕፍቲ ባይዱExtracts
Grinding leaves（100g-200ml dh2o)—Filtrate—Centrifuged （3000rpm,15min) — lyophilized—re-extracted （methanol）—collect the organic layer—evaporated— dry matter was dissolved （10ml dh2o ) —centrifuged （ 3000rpm,15min ）