多糖中糖醛酸含量测定方法的研究进展

食品研究与开发

F ood Research And Development

圆园19年9月第40卷第17期

多糖中糖醛酸含量测定方法的研究进展

李亚平1，周鸿立1，

2，*

(1.吉林化工学院化学与制药工程学院,吉林吉林132022;2.吉林省农业资源综合开发与利用工程

研究中心,吉林吉林132022)

摘要：综述多糖中糖醛酸含量测定的方法,以期为更准确的测定糖醛酸含量提供一定的参考。查阅近年国内外相关文献,对其含量测定的不同方法进行比较,并分析其各自的优缺点。常见方法有光谱法和色谱法。光谱法有吡唑-硫酸法、间羟基联苯法及3,5-二甲基苯酚法、色谱法有气相色谱法、分子体积排阻液相色谱法及阴离子液相色谱法。不同方法的测试条件及准确度各有差别。光谱法操作简单,但干扰因素多,测定结果偏离理论值较大,且测得的是总糖醛酸的含量;色谱法能够确地测定出不同种类的糖醛酸含量,其中高效阴离子液相色谱法不需要衍生化,是未来测定糖醛酸含量的主要方法。

关键词：多糖;糖醛酸;中性糖;含量测定;光谱法;色谱法

Progress on Determination Content of Uronic Acid in Polysaccharides

LI Ya-ping 1,ZHOU Hong-li 1,2,*

(1.College of Chemical and Pharmaceutical Engineering ,Jilin Institute of Chemical Technology ,Jilin 132022,Jilin ,China ;2.Engineering Research Center for Agricultural Resources and Comprehensive Utilization of Jilin

Province ,Jilin 132022,Jilin ,China )Abstract ：Methods for determination the content of uronic acid in polysaccharides were reviewed ，In order to provide some reference for the accurate determination of the content of uronic acid.By reviewing relevant literatures at home and abroad in recent years ，the characteristics of different methods were compared ，and analyzed their respective advantages and disadvantages.The common methods were spectroscopy and chromatography.The spectroscopy included pyrazole-sulfuric acid method ，hydroxybiphenyl method and 3，5-dimethylphenol method ，and the chromatography had gas chromatography ，molecular size exclusion liquid chromatography and anionic liquid chromatography.The test conditions and accuracy of different methods were different.The spectroscopy was simple to operate ，but there were many interference factors and large deviation and the measured result was the total uronic acid content.The chromatography could accurately determine

different kinds of uronic acid content ，among which the high-efficiency anion liquid phase chromatography did not require derivatization.It was the primary method for determination the uronic acid content in the future.Key words ：polysaccharide ；uronic acid ；neutral sugars determination content ；spectroscopic method ；chro -

matography

引文格式:

李亚平,周鸿立.多糖中糖醛酸含量测定方法的研究进展[J].食品研究与开发,2019,40(17):207-211

LI Yaping ,ZHOU Hongli.Progress on Determination Content of Uronic Acid in Polysaccharides[J].Food Research and Devel -opment ,2019,40(17):207-211

基金项目:吉林省省级产业创新专项资金项目(2017C044)

作者简介:李亚平(1991—),女(汉),硕士研究生,研究方向:天然产物的研究与开发。*通信作者:周鸿立(1967—),女(汉),教授,博士,研究方向:天然产物的研究与开发。

DOI :10.12161/j.issn.1005-6521.2019.17.036

专题论述

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