首次在长角血蜱中检测到汉赛巴尔通体

中国媒介生物学及控制杂志2015年2月第26卷第1期Chin J Vector Biol &Control,February 2015,Vol.26,No.1

蜱是最重要的疾病传播媒介之一，可以传播细菌（螺旋体、立克次体和弗朗西斯氏菌等）、病毒（黄病毒、Colti 病毒和内罗病毒等）和寄生虫等（巴贝西

虫等）［1］

。栗冬梅等［2］、孙继民等［3］、凌锋等［4］在我国

微小扇头蜱（Rhipicephalus microplus ）、中华硬蜱（Ixodes sinensis ）和长角血蜱（Haemaphysalis longicornis ）等蜱种中分离或检测到巴尔通体，并已

在猫、犬、啮齿类动物中分离或检测到巴尔通体［5］。巴尔通体的人类感染近年来在我国引起关注，特别是致病的汉赛巴尔通体（Bartonella henselae ）、五日热巴尔通体（Bartonella quintana ）等，由汉赛巴尔通体引起的猫抓病已有多起报道。现将采自石家庄市灵寿县长角血蜱中的汉赛巴尔通体自然感染情况报道如下。

·论著·

首次在长角血蜱中检测到汉赛巴尔通体

吴海霞1，李志芳1，2，3

，刘起勇1，张卫东2，栗冬梅1，马怀雷1，鲁亮1，刘京利1

1中国疾病预防控制中心传染病预防控制所媒介生物控制室，传染病预防控制国家重点实验室，世界卫生组织

媒介生物监测与管理合作中心，北京102206；2郑州大学公共卫生学院；3郑州大学附属洛阳中心医院

摘要：目的检测长角血蜱中汉赛巴尔通体的携带情况。方法

将采获于石家庄市灵寿县的长角血蜱分组，经无

菌处理后研磨匀浆，一部分直接提取DNA 进行PCR 检测，另一部分接种于含5%去纤维羊血的胰酶大豆培养基上，在37℃，5%CO 2的培养箱中进行培养后，挑取疑似菌落进行PCR 检测。对所得阳性条带的PCR 产物测序，并将所测核酸序列在GenBank 中进行序列比对。结果

菌落提取的DNA 样品中有2个样品出现阳性条带，但测序未得结果；

直接提取的DNA 样品中有1个样品出现阳性条带，经过同源性比对后为汉赛巴尔通体。结论石家庄市灵寿县采

集到的长角血蜱中存在汉赛巴尔通体感染，这是首次在长角血蜱中检测到汉赛巴尔通体。关键词：长角血蜱；汉赛巴尔通体；分离培养；聚合酶链反应中图分类号：R384.4；R183.7

文献标志码：A

文章编号：1003-4692（2015）01-0016-03

DOI ：10.11853/j.issn.1003.4692.2015.01.004

First detection of Bartonella henselae infection in Haemaphysalis

longicornis

WU Hai ⁃xia 1,LI Zhi ⁃fang 1，2，3

,LIU Qi ⁃yong 1,ZHANG Wei ⁃dong 2,LI Dong ⁃mei 1,

MA Huai ⁃lei 1,LU Liang 1,LIU Jing ⁃li 1

1WHO Collaborating Centre for Vector Surveillance and Management ,State Key Laboratory for Infectious Disease Prevention

and Control ,National Institute for Communicable Disease Control and Prevention ,Chinese Center for Disease Control and Prevention ,Beijing 102206,China;2Department of Epidemiology and Health Statistics ,College of Public

Health ,Zhengzhou University ;3Luoyang Central Hospital Affiliated to Zhengzhou University

Supported by the National Natural Science Foundation of China （No.30600513）and the National Science and Technology Major Project for Infectious Diseases in China （No.2012ZX10004-219）

Abstract:Objective To detect the infection with Bartonella henselae in Haemaphysalis longicornis .Methods Haemaphysalis longicornis captured in Lingshou county,Shijiazhuang,Hebei province,China,were divided into groups.The H.longicornis in each group was disinfected,sterilized,and grinded.Half of the grinded mixture was directly used for DNA extraction and polymerase chain reaction (PCR)analysis;the other half was inoculated onto tryptic soy agar supplemented with 5%defibrinated sheep blood followed by culturing in a 5%CO 2incubator at 37℃,and the suspected colonies were selected for PCR analysis.The PCR products that produced positive bands were sequenced,and the nucleotide sequences obtained were aligned with known sequences in GenBank.Results A positive band was observed in one sample from direct DNA extraction,the sequence of which was found to belong to B.henselae by alignment analysis.Positive bands were observed in two DNA samples isolated from bacterial cultures,but no results were produced from sequencing.Conclusion Haemaphysalis longicornis collected in Lingshou county,Shijiazhuang,are infected with B.henselae .This is the first detection of B.henselae infection in H.longicornis .

Key words:Haemaphysalis longicornis ;Bartonella henselae ;Culture;Polymerase chain reaction

基金项目：国家自然科学基金（30600513）；国家传染病科技重大专项（2012ZX10004-219）作者简介：吴海霞，女，博士，副研究员，主要从事蜱类相关研究，Email:wuhaixia@ 网络出版时间：2014-12-1711：21

网络出版地址：/kns/oldnavi/n_CNKIPub.aspx?naviid=59&BaseID=ZMSK&NaviLink=

·

·16