饲料原料的纤维

Xylose
Mannose
Galactose
Glucose Uronic acid
AVEVE Biochem - Ghent University Research
Weight percentage / RM
Fibre “finger print” determines enzyme concept
菜粕
GLUCANASE
XYLANASE
glucan
5
4
arabinoxylan
3
arabinan
2
1
pectins
0 Rhamnose
Fucose
Arabinose
Xylose
Mannose
Galactose
Glucose Uronic acid
Weight percentage / RM
PECTINASE
Fibre “finger print” determines enzyme concept
Aveve Group
棕榈粕
椰子粕
Fibre-degrading Enzymes in Pigs : Research Aveve - Ghent University
9
8
7
XYLANASE GLUCANASE
6
5
4
cellulose
3
arabinoxylan
2
1
0 Rhamnose
Fucose
Arabinose
Xylose
Mannose
Galactose
Glucose Uronic acid
Soy (22,00% NSP)
大豆
9
GLUCANASE
8
7
XYLANASE
消化位点与PH范围
Aveve Group
% rel. activiteit
pH profile xylanase activity in AveMix and competitor products
120%
100%
80%
60%
40%
20% 3
3,5
4
4,5
5
5,5
6
6,5
7
pH
Fungal Xylanase (Trichoderma spp.)
- Xylans are generally less soluble than glucans 通常，木聚糖可溶性低于葡聚糖。
- More insoluble fibres than soluble fibres 不可溶性纤维比可溶性纤维多。
- Insoluble fibres need higher enzyme activity ! 不可溶性纤维需要更高酶活。
2. Eliminate starch with amylase 除去淀粉含量
7
3. Recover the total dietary fibre fraction (TDF) 测定总日粮纤维含量
4. Hydrolyse the fibres with dilute acid to sugar monomers 水解纤维
20
18
GLUCANASE 16
14
GLUCANASE
Weight percentage / RM
XYLANASE
12
10
XYLANASE
8
6
4
2
Fucose
Arabinose
Xylose
Mannose
Galactose
Glucose Uronic acid
0 Rhamnose
Fucose
Arabinose
glucan
6
5
4
arabinoxylan
3
arabinan
pectins
2
1
0 Rhamnose
Fucose
Arabinose
Xylose
Mannose Galactose
Glucose Uronic acid
Weight percentage / RM
Weight percentage / RM
Uronic acid
0
Rhamnose
Fucose
Arabinose
Xylose
Mannose
Galactose
Glucose
Uronic acid
20 18 16 14 12 10
8 6 4 2 0
Rhamnose
Cotton seed (26,29% NSP)
棉粕
葵花粕 Sunflower (30,34% NSP)
Wheat (9,92% NSP)
小麦
9
Aveve Group
8
7
XYLANASE
Gபைடு நூலகம்UCANASE
6
5
4
3
arabinoxylan
2
glucan
1
0 Rhamnose
9 8 7 6
Fucose
Arabinose
Xylose
Mannose
Galactose
Glucose Uronic acid
Rapeseed (20,25% NSP)
xylan glucan
xylan glucan
xylan glucan
xylan glucan
xylan glucan
xylan glucan
xylan glucan
xylan glucan
100% 90% 80% 70% 60% 50% 40% 30% 20% 10% 0%
燕麦
Oa ts
大米
Rice
27 %
31 %
36 %
11 %
C ORN
玉米
WHEAT
小麦
SOYAMEAL
豆粕
WHEATBYPR.
C ORNGLUT.M
小麦副产品 玉米蛋白粉
Fiber- degrading Enzymes in Pigs- Project Aveve, Univ. of Ghent 1990- 1996
Percent (%) of total proteins linked to fibres (limiting CP digestibility of raw materials)
Weight percentage / RM Weight percentage / RM
15 14 13 12 11 10
9 8 7 6 5 4 3 2 1 0
Rhamnose
Fucose
Wheat bran (38,2% NSP)
麸皮
Arabinose
Xylose
Mannose Galactose
Aveve Group
原料中纤维结合蛋白的比例（限制蛋白质消化率的因素）
49%
27%
31%
36%
39%
33%
37%
31%
11%
15% 12%
Corn
Wheat
Soybean
Wheat meal
byCpororndugcltuteRnapmeseeael dTampeioacl a
Coconut mCoetatlonseeRdicme ebaral nSunflower meal
Glucose Uronic acid
Aveve Group
玉米
Corn (8,74% NSP)
9
8
7
6
5
4
3
2
1
0 Rhamnose
Fucose
Arabinose
Xylose
Mannose
Galactose
Glucose Uronic acid
米糠
中国玉米DDGS
Fibre-degrading Enzymes in Pigs : Research Aveve - Ghent University
Fibre types: “Fibre Fingerprint”
Aveve Group
小麦
Wheat (9,92% NSP)
9
8
7
XYLANASE
GLUCANASE
6
Weight percentage / RM
5
4
3
Arabinoxylan
=Arabinose
2
Glucan =Cellulose
1
纤维对消化位点迁移的影响
Aveve Group
AveMix® Enzymes: shift nutrient digestion to small intestine !
Nutrient monomers UPTAKE
LOSS of Amino-acids, phosphorus
Amino-acids, phosphorus, fats, sugars, … CONVERSION of sugars
49 %
- Delivery site of protein / AA in the animal ! - Effects of enzyme on nutrient delivery site : more proximal delivery - Impact of fibres on digesive system and nutrient losses
0
Rhamnose
Fucose
Arabinose
Xylose
Mannose
Galactose
Glucose Uronic acid
Weight percentage / RM
Fibre “finger print” determines enzyme concept
玉米
Corn (8,74% NSP)
6
5. Analyse the sugar composition with GC or colorimetric method用气
相色谱或比色方法分析糖的成分
5
6. The sugar profile is an indicator of the fibre types in the raw
4
material ( qualitative and quantitative ) 对糖分进行定性定量分析
大麦
小麦DDGS Wheat DDGS (25,77% NSP)
9
Aveve Group
XYLANASE
8
GLUCANASE 7
6
XYLANASE
GLUCANASE
Weight percentage / RM
5
4
3
2
1
Fucose
Arabinose
Xylose
Mannose
Galactose
Glucose
Cell Wall
Enzymes
Hydrophilic Storage Structure
Nutrients (e.g. wheat starch)
- 内源酶 - ”笼效应” - 纤维结合蛋白 - 养分消化位点 / 细菌发酵
β(1→3),(1→4) linked D-Glucan polymer β(1→4) linked D-Arabino- Xylan polymer
AVEVE Biochem - Ghent University Research
PECTINASE
Fibre “finger print” determines enzyme concept
Weight percentage / RM
9 8 7 6 5 4 3 2 1 0
Rhamnose
Barley (16,50% NSP)
Bacterial xylanase
真菌来源木聚糖酶
细菌来源木聚糖酶
纤维类型: “纤维指纹图谱”
Aveve Group
Corn (8,74% NSP) 英威酶：与纤维合理匹配
玉米
9
NSP-fibres: analysis of fibre sugar composition
8
1. Raw material sample 原料取样
饲料原料中的纤维
周勃 博士
Aveve Group
饲料原料中的纤维
Aveve Group
可溶/不可溶Soluble / insoluble – effect on digestion efficacy
不可溶纤维 Unsoluble = inclusion 可溶性Soluble Type = viscosity
Englyst (1989); Choct, University of New England, Australia
Feed Fibres : digestion of protein
饲料纤维：蛋白质的消化
Aveve Group
- Proteins in Fiber – 纤维中的蛋白
Fibre protein / Total Protein 纤维蛋白/总蛋白
大麦
Ba rle y
小麦
高粱
黑麦
Wheat
Sorghum
Rye
黑小麦 玉米
soluble insoluble
Tritica le
*
*
* Corn
* Glucan = cellulose
- Importance of glucans ( cellulose ) together with xylans !葡聚糖和木聚糖一样重要
Weight percentage / RM
3
2
1
0
Rhamnose
Fucose
Arabinose
Xylose
Mannose
Galactose
Glucose Uronic acid
鼠李糖 海藻糖 阿拉伯糖 木糖
AVEVE Biochem - Ghent University Research
甘露糖 半乳糖 葡聚糖 糖醛酸
- 持水率增加 - 内源酶损失 - 减缓食糜移动和养分消化吸收 - 采食量降低
原料中的纤维类型: 木聚糖和葡聚糖纤维的溶解度
谷物类原料中可溶性和不可溶性木聚糖和葡聚糖的比例
Aveve Group
Level of SOLUBLE et INSOLUBLE xylans and glucans in cereals