北京林业大学研究生学位论文模板

Supplemented with 10mg/L 2,4-D, suspension cultures initiated from callus derived from petiole of aspen hybrid 3# produced embryogenic callus and a highest number of globular-shaped embryos, and cotyledonary-stage embryos originated from white callus of grey aspen Ca(P.×canescens) shoot were first observed and embling were obtained. Genotypes and concentration of 2,4-D was critical to induce embryogenic and somatic embryogenesis. Morphogenesis of shoot was affected by culture temperature. Characteristic of callus produced embryogenic cell was heterogeneous, but potential ability to produce embryogenic cell was different.
2,4-D 高浓度悬浮培养下，成功的将美洲山杨与欧洲山杨杂种 3#叶柄愈伤组织调 节为胚性状态，通过细胞悬浮培养，获得了大量的球形胚，并首次观察到灰杨 Ca 茎 尖白色愈伤组织内起源的子叶胚形成，得到了完整植株。基因型和 2,4-D 浓度对愈伤 组织状态调节，至为关键。温度变化可影响茎尖形态发生。愈伤组织达到胚性状态时， 表面特征不一致，但有产生胚性细胞能力强弱之分。 关键词：白杨，茎尖培养，体细胞胚胎发生
Key words: Populus (aspen clones), shoot culture, somatic embryogenesis
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目录
目录
摘要............................................................................................................................................................ Ⅰ ABSTRACT............................................................................................................................................... Ⅱ 引言.............................................................................................................................................................. 1 1 文献综述................................................................................................................................................... 5 1.1 杨树茎尖培养研究进展........................................................................................................................5 1.1.1 杨树茎尖培养概述.............................................................................................................................7 1.1.2 杨树茎尖培养技术研究.....................................................................................................................9 1.1.2.1 培养基............................................................................................................................................ 10 1.1.2.2 外植体选择................................................................................................................................... 13 1.1.2.3 激素条件........................................................................................................................................ 15 1.2 杨树体细胞胚胎发生研究进展..........................................................................................................17 1.2.1 杨树体细胞胚胎发生概述...............................................................................................................20 图版............................................................................................................................................................ 23 参考文献.................................................................................................................................................... 26 个人简介.................................................................................................................................................... 31 导师简介.................................................................................................................................................... 33 致谢............................................................................................................................................................ 34
论文提交日期 2005 年 5 月
论文答辩日期 2005 年 6 月
学位授予日期
答辩委员会主席： 评 阅 人：
北京林业大学
独创性声明
本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成 果。尽我所知，除了文中特别加以标注和致谢的地方外，论文中不包括含其他人已经 发表或撰写过的研究成果，也不包括为获得北京林业大学或其它教育机构的学位或证 书而使用过的材料。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了 明确的说明并表示了谢意。
The results of study on aspen shoot culture using node as explant show that percent bud regeneration of AG (Populus alba×P. grandidentata) and 3# (P. tremuloides×P. tre- mula) was lower than that of the other clones. Percentage of bud regeneration could be improved at vertical cutting. With the increasing of zeatin concentration, in vitro organogenesis was different. All clones were easy to induce rooting, especially AG and Ca, and number of roots was fit to estimate the effect on rooting of plantlet.
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白杨无性系体茎尖培养及体细胞胚胎发生技术研究
Shoot Culture and Somatic Embryogenesis in Aspen Clones
Master Candiadate: Yu Zhishui (Specialty of Forestry Genetics and Tree Breeding)
签名：
Hale Waihona Puke Baidu
日期：
关于论文使用授权的说明
本人完全了解北京林业大学有关保留、使用学位论文的规定，即：学校有权保留 送交论文的复印件，允许论文被查阅和借阅；学校可以公布论文的全部或部分内容， 可以采用影印、缩印或其它复制手段保存论文。 (保密的论文在解密后应遵守此规定)
签名：
导师签名：
日期：
摘要
中文摘要
本论文采用茎尖培养三步法，首次对引入我国表现优良的 8 个白杨无性系，进行 了微体快繁，为今后系统的苗期测定及田间试验提供了材料。同时，选用了 5 个无性 系，以水培苗或组培无菌苗为材料，进行愈伤组织诱导或对其发育状态调控及体细胞 胚胎发生研究，为建立稳定的杨树体细胞胚胎发生体系奠定了基础。
分类号 密级 UDC
学位论文
白杨无性系茎尖培养及体细胞胚胎发生技术研究
(中文题名)
Shoot Culture and Somatic Embryogenesis in Aspen Clones
(英文题名)
ⅩⅩⅩ
(作者姓名)
指 导 教 师 ⅩⅩⅩ教授
申请学位级别 硕
士
学科专业名称 林木遗传育种
研 究 方 向生 物 技 术
Directed by Professor Zhang Zhiyi
ABSTRACT
In this paper, a three-step micropropagation was successfully employed for 8 selected genotypes of aspen. At the same time, through the induction of embryogenic callus, Somatic embryogenesis was studied. It would be beneficial to establish a stable system of embryogenesis in poplar.
试验结果表明，以水培苗带芽茎段为茎尖培养材料，银白杨与大齿杨杂种 AG 和 美洲山杨与欧洲山杨杂种 3#芽分化率显著低于其余 6 个无性系；垂直接种可显著提 高水培苗腋芽分化率；玉米素浓度对芽分化率影响不明显，但影响材料的离体器官发 生表现；8 个无性系皆易诱导生根，其中银白杨与大齿杨杂种 AG 和灰杨 Ca 较其它 无性系更易，选用根数作为评定生根效果指标更合适。