固定蛋白检测小分子-2015

Accepted Manuscript

Title:Dextran Hydrogel Coated Surface Plasmon Resonance

Imaging(SPRi)Sensor for Sensitive and Label-free Detection

of Small Molecule Drugs

Author:Shaopeng Li Mo Yang Wenfei Zhou Trevor G.

Johnston Rui Wang Jinsong Zhu

PII:S0169-4332(15)01118-6

DOI:/doi:10.1016/j.apsusc.2015.05.020 Reference:APSUSC30345

To appear in:APSUSC

Received date:8-3-2015

Revised date:3-5-2015

Accepted date:4-5-2015

Please cite this article as:S.Li,M.Yang,W.Zhou,T.G.Johnston,R.Wang,J.Zhu, Dextran Hydrogel Coated Surface Plasmon Resonance Imaging(SPRi)Sensor for Sensitive and Label-free Detection of Small Molecule Drugs,Applied Surface Science (2015),/10.1016/j.apsusc.2015.05.020

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A c c e p t e d M a n u s c r

i p t

Dextran Hydrogel Coated Surface Plasmon Resonance Imaging (SPRi) Sensor for Sensitive and Label-free Detection of Small Molecule Drugs

Shaopeng Li a, b ‡, Mo Yang a, c ‡, Wenfei Zhou a, c , Trevor G. Johnston d , Rui Wang a, c , Jinsong Zhu a,*

a National Center for NanoScience and Technology, Beijing 100190, China

b Department of Chemistry, Tsinghua University, Beijing 100084, China

c University of the Chinese Academy of Sciences, Yuquan Rd., 19(A), Beijing 100049, China d

Plexera LLC, Woodinville, Washington 98072, United States *Corresponding author: Jinsong Zhu, Tel.: +86-010-********, E-mail address: jizhu@ ‡

These authors contributed equally to this work Abstract The label-free and sensitive detection of small molecule drugs on SPRi is still a challenging task, mainly due to the limited surface immobilization capacity of the sensor. In this research, a dextran hydrogel coated gold sensor chip for SPRi was successfully fabricated via photo-cross-linking for enhanced surface immobilization capacity. The density of the dextran hydrogel was optimized for protein immobilization and sensitive small molecule detection. The protein immobilization capacity of the hydrogel was 10 times greater than a bare gold surface, and 20 times greater than an 11-mercaptoundecanoic acid (MUA) surface. Such a drastic improvement in immobilization capacity allowed the SPRi sensor to detect adequate response signals when probing small molecule binding events. The binding signal of 4 nM liquid-phase biotin to streptavidin immobilized on dextran surface reached 435 RU, while no response was observed