对羧基苯硼酸论文

本科毕业论文（设计、创作）

题目：对羧基苯硼酸-明胶纳米微球制备及抗肿瘤疗效研究

题目类别：□√论文□设计□ 创作

学生姓名：XXX 学号：XXXXXXX

所在院系：生命科学学院专业：生物技术

入学时间：2012年9月

导师姓名：王鑫职称/学位：教授

导师所在单位：安徽大学生命科学学院

完成时间：2016 年6月

安徽大学教务处制

对羧基苯硼酸-明胶纳米微球制备及抗肿瘤疗效研究

摘要

目标：以明胶为起始材料制备纳米微球，并在其表面修饰具有肿瘤靶向功能的小分子配体对羧基苯硼酸。方法：利用不良溶剂法制备不同明胶纳米微球，利用对羧基苯硼酸的羧基与明胶微球表面的氨基形成酰胺键制备靶向纳米微球。利用动态光散射研究了纳米微球的粒径分布以及在不同生理条件下的稳定性。利用动态光散射纳米激光粒度仪测量了纳米微球在不同pH值下的表面电荷变化。利用透射电子显微镜来察看了纳米微球的表形特征。负载抗肿瘤药物阿霉素，并探究了载药纳米微球的体外释放行为。结果：制备的明胶纳米微球及靶向纳米微球粒径在160 nm左右。稳定性实验分析数据表明纳米微球在不同生理环境下都具有良好的稳定性。体外药物释放实验证明负载阿霉素的纳米微球可以顺利的将药物释放出来，且释放行为具有一定的pH依赖性。细胞毒性实验证明载药纳米微球可以很好的抑制人结肠癌细胞的生长。

关键词：对羧基苯硼酸；明胶；纳米微球；抗肿瘤

4-Carboxyphenylboronic acid - gelatin nanoparticles preparation and

antitumor efficacy research

Abstract

Objective: Gelatin was chosen as an initial material to prepare nanoparticles. 4-Carboxyphenylboronic acid (4-CPBA) was then modified on the surface of gelatin nanoparticles to give tumor-targeting nanoparticle drug delivery systems. Methods: The size and distribution of these two nanoparticles were measured by dynamic light scattering (DLS). The micromorphology of nanoparticles was observed by transmission electronic microscope (TEM). Zeta potential of gelatin nanoparticles and 4-CPBA modified nanoparticles was measured by DLS. The stability of these two nanoparticles in different conditions were then investigated. Drug release from nanoparticles were measured at pH 5.0, 6.0 and 7.4. Cytotoxicity of these two nanoparticles were evaluated against HCT cell line. Results: TEM images display that gelatin nanoparticles and 4-CPBA modified nanoparticles are spherical like with a uniform diameter around 160 nm. Both tow nanoparticles display an extraordinary stability in different conditions. DOX was successfully loaded into NP1 and NP2, and DOX release rate from nanoparticles increase significantly with the change of pH value from 7.4 to 5.0. The results of MTT assay demonstrated that these DOX-loaded nanoparticles can efficiently inhibit the growth of HCT cells.

Key words: 4-Carboxyphenylboronic acid; Gelatin; nanoparticles; antitumor

目录

1 引言 (1)

1.1 明胶的基本概述 (1)

1.2 载药纳米微球的基本概述 (1)

1.3 肿瘤治疗的基本概述 (2)

1.4 论文选题意义 (2)

2 实验方法 (3)

2.1 材料及仪器 (3)

2.2 实验步骤 (3)

2.2.1 明胶以及4-羧基苯硼酸修饰的明胶纳米粒子的制备 (3)

2.2.2 NP1和NP2的粒径以及电位表征 (3)

2.2.3 NP1和NP2稳定性检测 (4)

2.2.4 NP1-DOX和NP2-DOX的制备 (4)

2.2.5药物释放 (4)

2.2.6 NP1-DOX和NP2-DOX的细胞毒性试验 (4)

3 结果讨论 (5)

3.1 NP1和NP2的粒径以及电位表征 (5)

3.2 NP1和NP2稳定性 (6)

3.3 药物释放 (7)

3.4 NP1-DOX和NP2-DOX的细胞毒性试验 (8)

4 结论 (10)

主要参考文献： (11)

致谢 (12)