荧光探针的发展与应用

Supplementary materials for:

Perylene diimide based “turn-on” fluorescence sensor for detection of

Pd2+ in mixed aqueous media

Hai-xia Wang a, b,*, Yue-he Lang a, Hui-xuan Wang a, Jing-jing Lou a, Hai-ming Guo a, b, Xi-you Li c,*

a School of Chemistry and Chemical Engineering, Key Laboratory of Green Chemical Media and Reactions of Ministry of Education, Henan Normal University, Xinxiang 453007, China

b School of Environmental Science, Henan Normal University, Xinxiang 453007, China

email: hxwang5270@ (H. Wang)

c School of Chemistry an

d Chemical Engineering, Shandong University, Jinan 250100, China email: xiyouli@ (X. Li)

Contents

Page S1: Title of the paper, authors along with the contents.

Page S2-S4: Copy of the 1H and 13C NMR spectra of PDI-1, PDI-2 and PDI-3.

Page S5:Photophysical properties of PDI-1, PDI-2and PDI-3derivatives in different solvents at room temperature; Fluorescence spectra change of PDI-2and PDI-3 upon addition of different metal(8.0 equiv) ions; UV-vis spectra of PDI-1 (6.0 μM) in the presence of different metal ions (8.0 equiv).

Page S6: Job’s plots in DMF/H2O (v/v, 7/1) and acetonitrile; Fluorescence spectra changes of PDI-1 (5.0 μM) in the presence of Pd2+in acetonitrile and chloroform;ESI mass spectra of PDI-1 in the presence of 1.0 equiv PdCl2 in CH3CN.

Page S7: Job’s plots of PDI-1 (5.0 μM)in the presence of Pd2+in chloroform; Influence of pH on fluorescence intensity of PDI-1 (5.0 μM) in the absence and presence of 1.0 eq Pd2+; Benesi-Hildebrand analysis results.

Fig. S1 1H NMR (400 MHz) spectrum of PDI-1 in CDCl3.

Fig. S213C NMR (100MHz) spectrum of PDI-1 in CDCl3.

Fig. S31H NMR (400 MHz) spectrum of PDI-2 in CDCl3.

Fig. S413C NMR (100 MHz) spectrum of PDI-2 in CDCl3.

Fig. S51H NMR (400 MHz) spectrum of PDI-3 in CDCl3.

Fig. S613C NMR (100 MHz) spectrum of PDI-3 in CDCl3.

Table S1: Photophysical properties of PDI-1, PDI-2 and PDI-3 derivatives in different solvents at room temperature

PDI-1 PDI-2 PDI-3

λabs m ax nm ㏒ε

M-1cm-1

λem

m ax

nm

Φ

F

λabs m ax㏒ελem m axΦF

nm M-1cm-1 nm

λabs

m ax

nm

㏒ε

M-1cm-1

λem

m ax

Φ

F

nm

Toluene 573 4.72 605 0.0062 572 4.58 603 0.080 572 4.64 604 0.49 DCE 577 4.70 613 0.021 577 4.53 608 0.083 577 4.63 611 0.44 DCM 578 4.70 614 0.010 578 4.52 614 0.090 578 4.63 613 0.50 THF 568 4.69 605 0.0043 569 4.65 598 0.082 567 4.63 600 0.36 Dioxane 567 4.73 596 0.0052 568 4.66 598 0.091 567 4.65 601 0.43 CH3CN 569 4.71 604 0.0063 569 4.64 604 0.079 568 4.09 601 0.39 DMSO 577 4.69 600 0.0030 578 4.57 611 0.041 577 4.40 614 0.24

Fig. S7 Fluorescence spectra change of PDI-2 (left) and PDI-3 (right) upon addition of different metal (8.0 equiv) ions including Cr3+, Mn2+, Fe3+, Co2+, Ni2+, Cu2+, Zn2+, Ag+, Cd2+, Hg2+, Pb2+ and Pd2+ in DMF/H2O (v/v, 7:1), λex = 540 nm, Slit: 2.5 nm; 5.0 nm.

Fig. S8 UV-vis spectra of chemosensor PDI-1 (6.0 μM)in the presence of different metal ions (8.0 equiv) of Mn2+, Co3+, Ni2+,Cu2+, Zn2+, Cd2+, Hg2+ and Pd2+ in DMF/H2O (v/v, 7:1). Inset showing the color change (left) before (right) after the addition of Pd2+ in visible light.