大鼠主动脉环张力的作用及机制

海州香薷总黄酮对离体大鼠主动脉环张力的作用及机制

徐佳1陈军津1 周育成1 王会平2

（1浙江大学医学院2004级临床医学专业3系3A班，浙江杭州 310058；

2浙江大学医学院生理教研室，浙江杭州310058）

[摘要] 目的：研究海州香薷总黄酮（total flavones from Elsholtzia splendens ，TFES）对离体大

鼠胸主动脉环收缩张力的作用及其机制。方法：采用大鼠离体胸主动脉灌流模型，累积加药，

检测海州香薷总黄酮对去氧肾上腺素（PE）和KCL预收缩的胸主动脉环收缩张力的影响。结果：海州香薷总黄酮对内皮完整的离体主动脉环基础张力的作用不明显（p>0.05）；内皮完整和去

内皮时，海洲香薷总黄酮（5、10、25、50、100、200ug/mL）对PE（10-4mol/L）和KCL（3mol/L）预收缩的血管环均产生舒张作用（p﹤0.05）；而用NO 合酶（NOS）抑制剂L-NAME（10-4mol/L），鸟苷酸环化酶（GC）抑制剂ODQ（10µmol/L）以及环氧酶（COX）抑制剂Indo（10-5mol/L）处理血管后，对低浓度及高浓度下海州香薷总黄酮舒张血管效应的阻断作用均不明显(p>0.05)；使用电压依赖性的K+通道阻断剂4-AP(1mmol/L)，Ca2+敏感性的K+通道阻断剂TEA(1mmol/L)

以及ATP 敏感性的K+通道阻断剂格列苯脲（Glib）(3µmol/L)处理后，对海州香薷总黄酮舒血

管效应的阻断作用亦均不明显；内皮完整和去内皮时，海州香薷总黄酮对PE（10-4mol/L）预收缩血管环的舒张作用均具有时间依赖性。结论：在低浓度及高浓度下，海州香薷总黄酮对大鼠

离体胸主动脉环产生的舒张效应并非通过NO-鸟苷酸环化酶途径，环氧合酶途径以及直接作用

于平滑肌细胞膜上电压依赖性的K+通道、Ca2+敏感性的K+通道、ATP 敏感性的K+通道的途径产生的，其中浓度下的舒张作用可能与鸟苷酸环化酶有关，其机制待进一步研究明确。

[关键词]海州香薷总黄酮;胸主动脉环;血管舒张;内皮

Effects and Mechanism of Total Flavonoids of Elsholtzia Splendens on Rat Thoracic aortic XU jia1,CHEN jun-jin1,ZHOU yu-cheng1，WANG hui-ping2

(1Clinical Medicine,2Department of Physioligy,Zhejiang University School of Medicine,Hangzhou 310058,China)

[Abstract] Objective：To investigate the effect of total flavones from Elsholtzia splendens（TFES）on rat thoracic aorta rings and the underlying mechanisms. Methods: The study was performed with the model of isolate rat thoracic aorta rings in organ bath, we investigate the effects of accumulated total flavones from Elsholtzia splendens on the constriction of high KCL and Phenylephrine(PE) preconstricted rat thoracicaorta with endothelium. Results: There is no effect of total flavones from Elsholtzia splendens (TFES) on the basal tonus in rat isolated aortic rings with endothelium(p>0.05).Total flavones from Elsholtzia splendens（5、10、25、50、100、200ug/mL）concentration dependently caused relaxation in vessels with or without endothelium precontracted both with 10-4mol/L phenylephrine (PE) and 60mmol/L KCL(p<0.05).However, the relaxation evoked by TFES of High and low concentration wasn’t inhibited by 10-4mol/L NG-nitro-L-arginine methyl ester (L-NAME)，10µmol/L guanyly cyclase inhibitor 1H-[1,2,4] oxadiazolo [4,3-alpha] quinoxalin-1-one (ODQ) and 10-5mol/L Indomethacin（Indo）(p>0.05). Also, the relaxation to the total flavones from Elsholtzia splendens was not inhibited by 1mmol/L tetraethylammonium (TEA) 、1mmol/L 4-aminopyridine（4-AP）and 3µmol/L Glibenclamide（Glib）. TFES time dependently caused relaxation in vessels with or without endothelium precontracted with 10-4mol/L phenylephrine (PE).Conclusion:The results indicate that TFES can relax the rat thoracic aorta rings with or without endothelium . At the same time, the relaxation by TFES of High and low concentration isn’t mediated by the NO-guanylyl cyclase pathway ,COX pathway and three kinds of K+-channels on the membrane of vascular smooth muscle,yet,the relaxation by TFES of mediate concentration may be mediated by GC,so the mechanisms need more research.

[Key words] TFES; thoracic aorta rings；vascular relaxation；endothelium