玉米穗行数全基因组关联分析

作物学报 ACTA AGRONOMICA SINICA 2014, 40(1): 1−6

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ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@

本研究由国家自然科学基金项目(31201219)和国家重点基础研究发展计划(973计划)项目(2011CB100106)资助。

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通讯作者(Corresponding authors): 翁建峰, E-mail: jfweng@; 李新海, E-mail: lixinhai@

第一作者联系方式: E-mail: zhanghuanxin150@

Received(收稿日期): 2013-06-19; Accepted(接受日期): 2013-09-16; Published online(网络出版日期): 2013-10-22. URL: /kcms/detail/11.1809.S.20131022.1730.016.html

DOI: 10.3724/SP.J.1006.2014.00001

玉米穗行数全基因组关联分析

张焕欣 翁建峰* 张晓聪 刘昌林 雍洪军 郝转芳 李新海*

中国农业科学院作物科学研究所 / 作物分子育种国家工程实验室, 北京100081

摘 要: 穗行数是玉米产量的重要组成性状, 其遗传解析对高产育种具有指导意义。本文以203份主要玉米自交系为材料, 2007年在新疆乌鲁木齐、吉林公主岭和海南三亚进行穗行数测定; 采用分布于玉米基因组的41 101个单核苷酸多态性(SNP)标记对穗行数进行关联分析。共鉴定出9个与穗行数显著关联(P < 0.0001)的SNP, 分别位于染色体框1.02、1.10、7.03、8.02、9.06和10.03。8个SNP 位于已定位的数量性状座位(QTL)区间内。在显著SNP 位点LD 区域内发掘出4个候选基因, 分别编码含F-box 结构域的生长素受体蛋白、玉米kn1蛋白、AP2结构域蛋白和富亮氨酸重复的跨膜蛋白激酶。采用全基因组关联分析策略发掘穗行数基因位点及候选基因, 将为克隆控制玉米产量性状基因奠定基础。

关键词: 玉米; 穗行数; 全基因组关联分析; 候选基因

Genome-wide Association Analysis of Kernel Row Number in Maize

ZHANG Huan-Xin, WENG Jian-Feng *, ZHANG Xiao-Cong, LIU Chang-Lin, YONG Hong-Jun, HAO Zhuan-Fang, and LI Xin-Hai *

Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Engineer Laboratory of Crop Molecular Breeding, Beijing 100081, China

Abstract: Kernel row number (KRN) is one of grain yield components in maize (Zea mays L.). Investigation of its genetic archi-tecture will help develop high-yield varieties in maize. In this study, the KRN in a panel of 203 maize inbred lines was detected in Urumqi of Xinjiang, Gongzhuling of Jilin, and Sanya of Hainan in 2007, and used to perform the genome-wide analysis for KRN using MaizeSNP50 BeadChip. A total of nine SNPs were found to be significantly associated with KRN at a threshold of P < 0.0001, which were on chromosome Bins 1.02, 1.10, 7.03, 8.02, 9.06, and 10.03, respectively. Eight of these SNPs were located in the QTL intervals reported previously. Meanwhile, four candidate genes were scanned, encoding auxin signaling F-box containing protein, kn1 protein, AP2 domain containing protein and leucine-rich repeat transmembrane protein kinase respectively. In sum-mary, these identified genes and SNPs will offer essential information for cloning yield-related genes in maize. Keywords: Maize; Kernel row number; Genome-wide association analysis; Candidate gene

玉米穗行数(kernel row number, KRN)形成于小穗分化期, 由小穗成对分生组织数目决定[1]。穗行数是决定玉米产量的主要构成因素, 属于数量性状, 广义遗传力较高[2], 其遗传解析对玉米高产育种具有指导意义。分子标记的发展使得QTL 作图成为解析穗行数遗传结构的有效方法[3]。目前, 关于穗行数定位研究报道较多, 影响穗行数的QTL 在玉米10条染色体上均有分布。Ma 等[4]利用综3×87-1构建

的294份重组自交系(recombinant inbred line, RIL)群体检测出13个穗行数QTL, 分别位于第1、第3、第4、第5、第8、第9和第10染色体。Lu 等[5]利用掖478×丹340的150个F 2:3家系共定位到13个控制穗行数的QTL, 位于染色体框7.03位点来自丹340的穗行数主效QTL qkrn7可解释平均表型变异17.86%。Guo 等[6]用郑58×昌7-2的231个F 2:3家系在两种播种密度下进行穗行数QTL 定位, 分别检测