磁性纳米颗粒MNPs

(scale bar: 10 µm). c) Bio-TEM images of
A549 cells incubated with IONP20-TAT-Tf (i–iii)
Peng H, Tang J, Zheng R, et al. Nuclear-Targeted Multifunctional Magnetic Nanoparticles for Photothermal Therapy.[J].
Applications
1.MRI and imaging applications
The figure shows T2-weighted MR images of dextran coated MNPs with different Fe concentrations. It is shown that the T2-weighted images’ brightness decreases significantly with the increase of Fe concentration. The observation reveals that dextran coated MNPs can be considered as a good MR T2 contrast agent under the T2-imaging sequences.
Applications
Applications
MRI and imaging applications Micro-NMR as point of care device
Targeted drug delivery with MNPs
Hyperthermia
SERS detection combined with magnetic trapping
the rising of the GDP of China, the morbidity rates of cancer rose to
0.314% from 0.286% and 0.181% in 2012 .
Chen W, Zheng R, Baade P D, et al. Cancer statistics in China, 2015.[J]. Ca A Cancer Journal for Clinicians, 2016, 66(2):115.
Khalkhali M, Sadighian S, Rostamizadeh K, et al. Synthesis and characterization of dextran coated magnetite nanoparticles
Applications
2.Magnetic nanoparticles in cancer therapy
Khalkhali M, Sadighian S, Rostamizadeh K, et al. Synthesis and characterization of dextran coated magnetite nanoparticles
Applications
1.MRI and imaging applications
Peng H, Tang J, Zheng R, et al. Nuclear-Targeted Multifunctional Magnetic Nanoparticles for Photothermal Therapy.[J].
Applications
The optical and photothermal properties of IONPs. a) The vis–NIR absorbance spectra of IONP-20, IONP-11, and IONP-5 with a concentration of 100 µg mL−1. b) The photothermal effects of IONP-20, IONP-11, and IONP-5 aqueous dispersions (100 µg mL− 1) were measured by laser irradiation (808 nm, 3 W cm−2). c) The photothermal heating curves of I O N P - 2 0 a n d I O N P - 11 a t d i f f e r e n t concentrations under 808 nm laser irradiation. d) The change in photothermal effect of IONP-20 and IONP-11 particles as a function of storage time (3 W cm−2, 100 µg mL−1, 500 s). The inset photos show the color change of IONP-20, IONP-11, and IONP-5 after 112 d.
Fuan A. The use of magnetic nanoparticles in cancer theranostics: Toward handheld diagnostic devices[J]. Biotechnology Advances, 2016, 34(4):354-361.
The synthesis of IONP-TAT-Tf and nuclear targeted strategy for effective cancer photothermal therapy.
Peng H, Tang J, Zheng R, et al. Nuclear-Targeted Multifunctional Magnetic Nanoparticles for Photothermal Therapy.[J].
Applications
TEM images and magnetic characterization of IONPs. a,b) TEM images of monodisperse iron oxide nanocrystals. a) IONP-20; b) IONP-11;
d) Magnetization loops of IONP-20, IONP-11, and IONP-5 particles. e) T1 (upper)/T2 (lower)-weighted MR images of IONPs particles dispersions with different concentrations
Applications
a) CLSM images of IONP-20-Tf and IONP-20-TAT-Tf incubated A549 cancer cells after irradiation by the 808 nm laser at 3 W cm−2 for 5 min. The cells were costained by calcein-AM and propidium iodide before imaging. b) Flow cytometry graphs of A549 cells treated by IONP-20, IONP-20Tf, IONP-20-TAT, and IONP-20-TAT-Tf with a concentration of 50 µg mL−1 (NIR laser λ = 808 nm, 1.5 W cm−2, 300 s).
Peng H, Tang J, Zheng R, et al. Nuclear-Targeted Multifunctional Magnetic Nanoparticles for Photothermal Therapy.[J].
Applications
The intracellular trafficking p r o c e s s o f I O N P - 2 0 - TAT- T f i s following : (I) enter the cell via Tf receptormediated endocytosis; (II)escape endosomal/lysosomal pathways; (III) target to nucleus due to the TAT peptides.
Applications of magnetic nanoparticles in cancer theranostics
Background
According to the report , in 2015, there are 4292000 new cases of cancer in China, and the death tolls had reached into 2814000.With
the mortality rate rose to 0.206% from
CONTENT
01
02 Applications
03 Conclusion
Introduction
Introduction
Introduction
Preparation Physical methods, Wet chemical
preparation methods, Microbial methods.
Surface
The core-shell structure of functionalized magnetic nanoparticles.
The agglomeration is caused easily in the process of preparation and application because of its higher surface energy. Once produced, the synthesized MNPs are typically surface-coated to improve their colloidal stability, increase their water-dispersibility, and provide chemical functionality for bioactive molecule additions. Physical Properties small particle diameter， large specific surface ，superparamagnetic， magnetic responsibility.
T1 relaxation rate plotted as a function of Fe concentration (mM) for dextran coated MNPs.
T2 relaxation rate plotted as a function of Fe concentration (mM) for dextran coated MNPs.
Applications
Intracellular colocalization of A549
cells incubated with IONP-20-TAT-Tf at
different incubate time. IONP-20-TAT-Tf is labeled with by FITC fluorescence from FITC-C6-TAT in cells (green). The endosome and lysosomes of A549 cells are stained with Dextran, Alexa Fluor 594 (red) and Lysotracker (blue), respectively
T2-weighted MRI images (1.5T, spin-echo sequence: repetition time TR = 1600 ms, echo time TE = 79 ms) of the dextran coated MNPs at various iron concentrations at 25°C.
Schematic diagram of endocytosis and nuclear-targeted of IONP-20-TAT-Tf.
Peng H, Tang J, Zheng R, et al. Nuclear-Targeted Multifunctional Magnetic Nanoparticles for Photothermal Therapy.[J].