人脑微血管内皮细胞

Human Brain Microvascular Endothelial Cells

(HBMEC)

Catalog Number: 1000 Cell Specification

The brain microvascular endothelial cells (MEC) are the major element of the blood-brain barrier and comprise the primary limitation to passage of substances, both soluble and cellular, from the blood into the brain. Brain MEC utilizes unique features that distinguish themselves from those of peripheral endothelial cells. Most prominent among these are the following: 1) intercellular ‘tight junctions’ that display high transendothelial electrical resistance and retard paracellular flux [1], 2) absence of fenestrae and a reduced level of fluid-phase endocytosis [2], and 3) asymmetrically-localized enzymes and carrier-mediated transport systems that engender a truly ‘polarized’ phenotype [3]. Like peripheral endothelial cells, however, brain MEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. In view of the organ specificity of MEC, Brain MEC has been widely used for studying the molecular and cellular basis of blood-brain barrier. HBMEC from ScienCell Research Laboratories are isolated from human brain tissue. HBMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HBMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HBMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBMEC are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HBMEC in vitro.

Product Use

HBMEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Crone, C. and Oleson, S. P. (1992) Electrical resistance of brain microvessel endothelium. Brain Res. 241: 49-

55.

[2] Reese, T. S. and Karnovsky, M. J. (1967) Fine structural localization of blood-brain barrier to exogenous

peroxidase. J. Cell Biol. 34:9-14.

[3] Vorbrodt, A. W. (1988) Ultrastructural cytochemistry of blood-brain barrier endothelia. Prog. Histochem.

Cytochem. 18(3):1-96.