基因工程学
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10. Animals can be genetically altered
11. Transgenic plants are important for agriculture
Teachers：
Chang-Sheng DU (杜昌升老师)：Preparation of DNA/RNA; Electrophoresis and hybridization of DNA/RNA; Vectors; Restriction enzymes and ligase; PCR and qPCR
Question: Insulin is a crucial drug for treatment of type I diabetes, please design a procedure to produce active insulin.
Tel: 65988634 E-mail: zhan-yun.guo@tongji.edu.cn
ห้องสมุดไป่ตู้
1. Cleavage of DNA at specific sites by restriction enzymes
2. Ligation of DNA fragments by DNA ligase
Genetic Engineering: A Brief Introduction
What is Genetic Engineering? Manipulation of DNA and RNA.
Zhan-Yun GUO, Ph.D.
Professor of Institute of Protein Research, Tongji University
Zhan-Yun GUO (郭占云老师)：From DNA sequence to protein product
Hua YANG (杨桦老师)：Animal (mice) engineering
How to learn this class：
1、Basic Acknowledge
2、Application of the knowledge
3. Amplification of the cloned DNA fragment
4. Proteins can be recombinantly expressed in host cells
5. DNA fragment can be selectively amplified by PCR
6. DNA molecules can be separated by gel electrophoresis
7. DNA can be rapidly sequenced
8. cDNA can be synthesized based on mRNAs
9. Gene can be mutated by sitedirected mutagenesis