DIAGNOSIS OF DISEASES
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申请人：GRONOWITZ JAN SIMON,KAELLANDER CLAS FREDRIK R
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摘要：A method of determining dTk isoenzyme levels of a human or animal body fluid or cell sample is disclosed, which comprises the steps of reacting said sample with a substrate for said isoenzyme in the presence of a phosphate donor and a buffer system, and measuring the amount of phosphorylated product formed, said amount being proportional to said isoenzyme level. The method is characterized by using in combination… a) a buffer system at pH ranging from 5 to 9 and being present in a concentration of no more than 250 mM,… b) a substrate consisting of 2 min -deoxy-5halouridin, part of which has a radio-labelled 5-halogen, and being present in a concentration ranging from 2 x 10<-><9> - 5 x 10<-><6>M, and… c) the phosphate donor being present in a concentration not exceeding 20 mM. …The sensitivity of the method permits measurement of minute amounts of dTk, and the method can be used for e.g. diagnosis, prognostics and monitoring of diseases involving alterated ATP mediated dTk activity, such as cancers, tumours, and certain virus infections, or alterated CTP mediated dTk activity, such as HSV type 1, type 2, and VZV infections.
IS OF DISEASES 发明人：GRONOWITZ, JAN-SIMON,KALLANDER,
CLAS FREDRIK RUNESSON 申请号：AU1554 083 申请日：19830510 公开号：AU1554 083A 公开日：19831202
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摘要：A method of determining dTk isoenzyme levels of a human or animal body fluid or cell sample is disclosed, which comprises the steps of reacting said sample with a substrate for said isoenzyme in the presence of a phosphate donor and a buffer system, and measuring the amount of phosphorylated product formed, said amount being proportional to said isoenzyme level. The method is characterized by using in combination… a) a buffer system at pH ranging from 5 to 9 and being present in a concentration of no more than 250 mM,… b) a substrate consisting of 2 min -deoxy-5halouridin, part of which has a radio-labelled 5-halogen, and being present in a concentration ranging from 2 x 10<-><9> - 5 x 10<-><6>M, and… c) the phosphate donor being present in a concentration not exceeding 20 mM. …The sensitivity of the method permits measurement of minute amounts of dTk, and the method can be used for e.g. diagnosis, prognostics and monitoring of diseases involving alterated ATP mediated dTk activity, such as cancers, tumours, and certain virus infections, or alterated CTP mediated dTk activity, such as HSV type 1, type 2, and VZV infections.
IS OF DISEASES 发明人：GRONOWITZ, JAN-SIMON,KALLANDER,
CLAS FREDRIK RUNESSON 申请号：AU1554 083 申请日：19830510 公开号：AU1554 083A 公开日：19831202