研究领域蛋白质组学

纪建国 教授
Jianguo Ji, PhD
Professor of Biochemistry and Molecular Biology
研究领域：蛋白质组学
主要从事衰老及其退行性病变的功能蛋白质组学研究。

利用体内外模型通过蛋白质组分析，从线粒体功能损伤、蛋白质翻译后修饰、小分子药物调节后关键蛋白质表达的动态变化、细胞核蛋白质复合物及组蛋白质动态修饰与功能关系揭示衰老及其退行性病变等细胞病变的发病机制，为重大疾病的预诊、干预和治疗提供创新性的理论依据。

主要研究内容:
1.线粒体功能损伤、蛋白质翻译后修饰及小分子化合物调控的分子基础
2.细胞核蛋白质复合物及组蛋白质动态修饰与功能关系
3.蛋白质组学新技术新方法
Research Fields: Proteomics
Our group mainly focuses on brain aging and neurodegenerative diseases by functional proteomics technology. We had built up the cell culture and animal models, sample preparation and separation, as well as high qualified mass spectrometry platform. 2-DE, 2-D LC coupled with MALDI TOF/TOF MS, LTQ Orbitrap MS and FT ICR MS were used to find the proteome expression changes and their modifications. We also utilize affinity-based enrichment and isotope labeling strategy for the qualitative identification and quantitative analysis of protein modification sites. In order to decipher the mechanism underlying brain aging and neurodegenerative diseases, we studied the dynamic changes of mitochondria dysfunction, protein post-translational modification, small molecular regulation, nuclear protein complex and histone modification using models in vivo or in vitro.
Representative Publications:
1.Liu Y, He J, Wang Q, Ji S, Pu H, Jiang T, Meng L, Yang X, Ji J. 2008. Comparative studies of
early liver dysfunction in senescence-accelerated mouse using mitochondrial proteomics approaches. Mol Cell Proteomics 7: 1755-1762
2.Xie X, Li S, Liu S, Lu Y, Shen P and Ji J, 2008. Proteomic analysis of mouse islets after
multiple low dose streptozotocin injection. BBA: Proteins and Proteomics 1784: 276-284.
3.He J, Liu Y, He S, Wang Q, Pu H, Tong Y and Ji J, 2007. Comparison of two-dimensional gel
electrophoresis based and shotgun strategies in the study of plasma membrane proteome.
Proteomics-Clinical Application 1: 231-241.
4.He J, Liu Y, He S, Wang Q, Pu H and Ji J, 2007. Proteomic analysis of a membrane skeleton
fraction from human liver. Journal of Proteome Research 6: 3509-3518.
5.Diao W, Chen W, Wu Y, Liu P, Xie X, Li S, Shen P, Ji J, 200
6. Serum, liver, and kidney
proteomic analysis for the alloxan-induced type I diabetic mice after insulin gene transfer of naked plasmid through electroporation. Protoemics 6: 5837-5845.
6.He S, Wang Q, He J, Pu H, Yang W and Ji J, 2006. Proteomic analysis and comparison of the
biopsy and autopsy specimen of human brain temporal lobe. Proteomics 18: 4987-4996.
7.Yang W, Liu P, Liu Y, Wang Q, Tong Y and Ji J, 2006. A proteomics profiling of rat
pheochromocytoma PC12 cells, Proteomics, 10:2982-2990.
8.Chen T, Wang Q, Cui J, Yang W, Shi Q, Ji J and Shen P, 2005. Induction of apoptosis in mice
liver by microcystin-LR: a combined transcriptomic, proteomic and simulation strategy.
Molecular & Cellular Proteomics 4: 958-974.
9.Zhou B, Yang W, Ji J and Ru B, 2004. Differential display proteome analysis of PC-12 cells
transiently transfected with metallothionein-3 gene. Journal of Proteome Research 3: 126-131. 10.Zhao R, Ji J, Tong Y, Pu H, Ru B, 2003. Use of serological proteomic methods to find
biomarkers associated with breast cancer. Proteomics 3: 433-439.
Contact:
Jianguo Ji, Ph.D. and Professor
The National Laboratory of Protein Engineering and Plant Genetic Engineering
College of Life Sciences
Peking University
Beijing 100871
P. R. China
Tel: 86-10-62755470 Fax: 62751526
E-mail: jijg@, proteomicsjjg@.
Group Members:
Yanfei Ren, Jingquan Dai, Hai Pu, Qingsong Wang, Jing Wu, Xiao Zou, Lu Luo, Mingrui An, Wenjia Lai, Xuyang Zhao, Hongyan Shen, Xiaoqing Ren, Yuanpeng Tong
Former Lab Members:
1.Rui Zhao, University of Wisconsin, Postdoctoral
2.Wen Chen, Medical School, Harvard University, Postdoctoral
3.Bo Zhou, Medical School, Harvard University, Postdoctoral
4.Xuedong Wang, NIH, Bethesda, Postdoctoral
5.Wei Yang, Medical School, Harvard University, Research Fellow
6.Sizhi He, Company
7.Weiqiang Chen, Viena Medical University, Postdoctoral
8.Weifei Diao, Viena Medical University, Postdoctoral
9.Yan Yan, University of Melburne, Postdoctoral
10.Xiaolei Xie, University of Michigan, Postdoctoral
11.Jintang He, University of Michigan, Postdoctoral
12.Yashu Liu, University of Michigan, Postdoctoral
13.Peng Liu,Connect & Develop, BJTC,P&G Bioscience, Asia
14.Shuai Li, Cornell University, Ph.D. Student
15.Qian Wang, School of Medicine, New York University, Ph.D. Student
16.Siyu Liu, School of Law, Peking University, Ph.D. Student
17.Hai Pu, Peking University, Postdoctoral
18.Qingsong Wang, Peking University, Postdoctoral
19.Xin Guan, Iowa State University, Ph.D. Student
20.Ting Chen, Medical School, Harvard University, Postdoctoral。