DUAL FUNCTION PRIMERS FOR AMPLIFYING DNA AND METHO
- 1、下载文档前请自行甄别文档内容的完整性，平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益，请联系客服反馈,我们会尽快为您处理(人工客服工作时间：9:00-18:30)。
专利名称：DUAL FUNCTION PRIMERS FOR
AMPLIFYING DNA AND METHODS OF USE 发明人：BEHLKE, MARK A.,WALDER, JOSEPH A.
申请号：EP06846404
申请日：20061130
公开号：EP2054530A4
公开日：
20091111
专利内容由知识产权出版社提供
摘要：The present invention provides novel nucleotide compositions that enable the detection of DNA synthesis products and methods for use thereof. In one embodiment, the method can be used in PCR and allows the progress of the reaction to be monitored as it occurs. In one embodiment, the invention employs at least one fluorescence-quenched oligonucleotide that can prime DNA extension reactions. In a second embodiment, the invention employs at least one fluorescence-quenched oligonucleotide that can function as a template for DNA extension reactions. In both embodiments, the oligonucleotide also functions as a probe for detecting the progress of successive extension reaction cycles. Signal detection is dependent upon DNA synthesis and can occur with or without probe cleavage.
申请人：INTEGRATED DNA TECHNOLOGIES, INC.
更多信息请下载全文后查看。