JNK信号介导TNF-α对内皮细胞VCAM-1的诱导表达

JNK信号介导TNF-α对内皮细胞VCAM-1的诱导表达
黄春;吴文伟;徐春平;窦萍
【期刊名称】《中国老年学杂志》
【年(卷),期】2009(029)014
【摘要】目的探讨肿瘤坏死因子(TNF)-α对内皮细胞血管细胞黏附分子-
1(VCAM-1)表达和分泌的影响,及其可能的细胞内信号途径.方法用10ng/ml TNF-α与人主动脉内皮细胞(HAEC)共培养不同时间,蛋白印迹法检测细胞VCAM-1蛋白表达和丝裂原活化蛋白激酶ERK1/2、INK的磷酸化水平,ELISA法测定条件培养液中可溶性VCAM-1(sVCAM-1)含量;分别用MEK-ERK1/2抑制剂PD98059和JNK抑制剂SP600125预处理HAEC 1h,观察TNF-α对VCAM-1表达和分泌的影响.结果 TNF-α以时问依赖方式诱导HAEC VCAM-1蛋白表达,4 h达最大诱导表达,为对照组的(5.80±0.44)倍,条件培养液中sVCAM-1的含量与其蛋白表达呈平行变化;SP600125(而非PD98059)显著抑制上述诱导效应.结论 TNF-α诱导HAEC VCAM-1的表达和分泌至少部分是通过JNK信号介导的.%Objective To investigate the effect of TNF-α on vascular cell adhesion molecule-1 (VCAM-1) expression and secretion in human aortic endothelial cell (HAEC), and the probable intraeeilular signaling pathway. Methods HAECs were incubated with 1O ng/ml TNF-α for various periods, VCAM-1 protein expression, phosphorylation of ERK1/2 and JNK were detected by Western blot. Sol-uble VCAM-1 (sVCAM-1) in the conditioned media was detected by ELISA. HAECs were pre-incubated with PD98059 (MEK-ERK1/2 in-hibitor) or SP600125 (JNK inhibitor) for 1 h, subsequently with TNF-α for 4 h,
expression and secretion of VCAM-1 were assessed. Re-stilts TNF-α induced expression of VCAM-1 protein in a time-dependent manner, with a peak at 4 h (5.80 0. 44 folds vs control group).The level of sVCAM-1 in the conditioned media was parallel to the protein expression. SP600125, but not PD98059, reduced TNF-α-induced expression of VCAM-1. Conclusions TNF-α-induced expression of VCAM-1 in HAECs is mediated by JNK signaling at least partly.
【总页数】3页(P1740-1742)
【作者】黄春;吴文伟;徐春平;窦萍
【作者单位】福建省老年医学研究所,福建医科大学附属协和医院干部病房,福建,福州,350001;福建省老年医学研究所,福建医科大学附属协和医院干部病房,福建,福州,350001;福建省老年医学研究所,福建医科大学附属协和医院干部病房,福建,福州,350001;福建省老年医学研究所,福建医科大学附属协和医院干部病房,福建,福州,350001
【正文语种】中文
【中图分类】R543
【相关文献】
1.丹皮酚对脂多糖诱导的大鼠血管内皮细胞VCAM-1、TNF-α释放及TLR4/NF-κB信号通路的影响 [J], 杨黎;戴敏;陈鹏;
2.青藤碱抑制TNF-α诱导人脐静脉内皮细胞VCAM-1表达 [J], 黄建林;林灼锋;罗敏琪;古洁若;余步云
3.丹皮酚对脂多糖诱导的大鼠血管内皮细胞VCAM-1、TNF-α释放及TLR4/NF-
κB信号通路的影响 [J], 杨黎;戴敏;陈鹏
4.3种中药复方血清对脂多糖诱导的人脐静脉内皮细胞炎症因子LOX-1、TNF-α、VCAM-1和ICAM-1表达的影响 [J], 姜华;姜玉姬
5.黄芪水提取物对TNF-α诱导的小鼠动脉内皮细胞VCAM-1表达的影响 [J], 游洋;段岩;张效林;康建;闫承慧;张秀莉;丰加涛;韩雅玲
因版权原因，仅展示原文概要，查看原文内容请购买。