黄连素对大鼠心脏缺血再灌注损伤的保护作用

黄连素对大鼠心脏缺血再灌注损伤的保护作用
张婷
【期刊名称】《贵州医药》
【年(卷),期】2018(042)005
【摘要】目的探讨黄连素(berberine,BBR)对大鼠心脏缺血再灌注损伤的作用及
其机制.方法将SD大鼠随机均分为假手术组(Sham)、心脏缺血/再灌注损伤组(IRI)和黄连素(低,中,高)组(BBR)(n=10).通过结扎左冠状动脉前降支构建大鼠MI/R模型.检测各组乳酸脱氢酶(LDH),肌酸激酶同工酶(CK-MB)、天门冬氨酸氨基转移酶(AST)、JAK2、STAT3、p-JAK2、p-STAT3、p-p65、p65、白介素-6(IL-6)、白介素1((IL-1β)和肿瘤坏死因子-α(TNF-α)的表达及心脏病理形态改变.结果与Sham组相比,IRI组CK-MB、AST、LDH、p-JAK2、p-STAT3、p-p65、IL-6、
IL-1β和TNF-α的表达以及心脏病理改变均明显增加(P<0.05),而JAK2、STAT3
和p65表达无差异(P>0.05).与IRI组相比,BBR组CK-MB、AST、LDH、p-JAK2、p-STAT3、p-p65、IL-6、IL-1β和TNF-α的表达以及心脏病理改变均明显减少(P<0.05),但JAK2、STAT3和p65表达无差异(P>0.05).结论黄连素预处理可减轻心脏缺血再灌注损伤,其作用机制与黄连素抑制JAK2/STAT3/NF-KB信号通路激活介导的炎症相关.%Objective To investigate the effect and mechanism of Berberine to myocardial ischemia reperfusion in-jury in rats.Methods 50 SD rats were randomly equally divided into Sham group (Sham),myocardial ischemia reperfusion injury group (MI/R),and Berberine
(low,middle,high)pretreatment group (BBR)(n=10).The model of IRI was induced by ligating left anterior descending coronary artery.The cardiac
pathology and the expres-sion of CK-MB,AST,LDH,p-JAK2,p-STAT3,p-
p65,IL-6,IL-1,and TNF-in the rats of all groups were ex-amined.Results Compared with Sham group,the IRI group had higher expression of CK-MB,AST,LDH,p-JAK2,p-STAT3,p-p65,IL-6,IL-1 and TNF-with higher change of cardiac pathology and there were no statistical significantly differences on the expression of JAK2,STAT3 and pared with IRI group,the BBR groups had lower expression of CK-MB,AST,LDH,p-JAK2 ,p-STAT3,p-
p65,IL-6,IL-1 and TNF-with lower change of cardiac pathology and there were no statistical significantly differences on the expression of
JAK2,STAT3 and p65.Conclusion Berberin can pretreatmentprotect rat against myocardial ischemia reperfusion injury.The mecha-nism of which is associated with suppressing JAK2/STATA3/NF-KB signal pathway mediating inflammation.
【总页数】4页(P522-525)
【作者】张婷
【作者单位】资阳市第一人民医院急诊科,四川资阳 641300
【正文语种】中文
【中图分类】R816.2;R322.1
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