MEASUREMENT OF BETA-ENOLASE
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专利名称：MEASUREMENT OF BETA-ENOLASE 发明人：KATOU KANEFUSA 申请号：JP22151882 申请日：19821216 公开号：JPS59111062A 公开日：1984 0627
摘要：PURPOSE:To improve measuring sensitivity, by a method wherein beta-enolase to be examined and enzyme labeled anti-betabeta-enolase antibody are immobilized by a solid phase through anti-betabeta-enolase antibody and, thereafter, the activity of the label enzyme bonded to the solid phase is measured. CONSTITUTION:An anti-betabetaenolase antibody is obtained by a method wherein a mammal such as a rabbit or a goat is immunized with betabeta-enolase isozyme and the serum thereof is subjected to salting out. In addition, as label enzyme, beta- D-galactosidase or alkali phosphatase is used while, as the bonding method of the label enzyme and the betabeta-enolase antibody, a method not deactivating the respective activities of the label enzyme and the antibetabeta-enolase antibody may by employed and a polyfunctional reagent such as glutaraldehyde or N,N-o- phenylmaleimido can be used. As a solid phase for insolublizing an anti-betabeta- enolase antibody, polysaccharides such as agarose, synthetic resin such as polystyrene, glass or polyacrylamide are used. By this method, measurement with high sensitivity and preciseness is enabled without receiving the influence of other components in live body fluids in an object to be examined.
专利名称：MEASUREMENT OF BETA-ENOLASE 发明人：KATOU KANEFUSA 申请号：JP22151882 申请日：19821216 公开号：JPS59111062A 公开日：1984 0627
摘要：PURPOSE:To improve measuring sensitivity, by a method wherein beta-enolase to be examined and enzyme labeled anti-betabeta-enolase antibody are immobilized by a solid phase through anti-betabeta-enolase antibody and, thereafter, the activity of the label enzyme bonded to the solid phase is measured. CONSTITUTION:An anti-betabetaenolase antibody is obtained by a method wherein a mammal such as a rabbit or a goat is immunized with betabeta-enolase isozyme and the serum thereof is subjected to salting out. In addition, as label enzyme, beta- D-galactosidase or alkali phosphatase is used while, as the bonding method of the label enzyme and the betabeta-enolase antibody, a method not deactivating the respective activities of the label enzyme and the antibetabeta-enolase antibody may by employed and a polyfunctional reagent such as glutaraldehyde or N,N-o- phenylmaleimido can be used. As a solid phase for insolublizing an anti-betabeta- enolase antibody, polysaccharides such as agarose, synthetic resin such as polystyrene, glass or polyacrylamide are used. By this method, measurement with high sensitivity and preciseness is enabled without receiving the influence of other components in live body fluids in an object to be examined.