苹果矮化砧木‘sh6’组培快繁培养基大量元素配方的优化
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果 树 学 报 2020，37（1）：40-49 Journal of Fruit Science
DOI:10.13925/ki.gsxb.20190213
苹果矮化砧木‘SH6 ’组培快繁培养基 大量元素配方的优化
杨雨璋，周贝贝，李民吉，李兴亮，张军科，张 强，魏钦平*
（北京市林业果树科学研究院·农业农村部华北地区研究中 心，北京 100097）
YANG Yuzhang，ZHOU Beibei，LI Minji，LI Xingliang，ZHANG Junke，ZHANG Qiang，WEI Qinping*
(Beijing Academy of Forestry and Pomology Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs/Beijing Engineering Research Center for Deciduous Fruit Trees, Beijing 100097, China)
mg·L-1，培养 35 d 继代系数 4.2，幼苗高度 2.6 cm。
关键词：苹果矮化砧木‘；SH6’；组织培养；大量元素；二次回归正交旋转设计；配方优化
中图分类号：S661.1
文献标志码：A
文章编号：1009-9980（2020）01-0040-10
Optimization of macro- element composition in the medium for in vitro propagation of apple dwarfing rootstock‘SH6’
KH2PO4 > CaCl2 · 2H2O，影响幼苗高度的主次顺序是 KNO3 > NH4NO3 > CaCl2 · 2H2O > MgSO4 · 7H2O > KH2PO4。因子交 互效应表明 ，继代系数受 NH4NO3 与 CaCl2 · 2H2O 交互效应的影响 ；幼苗高度受 KNO3 与 NH4NO3、KNO3 与 KH2PO4、 KNO3与 CaCl2·2H2O 交互效应的影响。配方优化的研究表明，理论预测与实验验证的结果无显著差异。【结论】MS 培
Abstract：【Objective】‘SH6’, a representative Chinese apple dwarfing rootstock, was used in this study to investigate the effect of macro-elements in tissue culture medium on in vitro subculture, in order to improve the efficiency of tissue culture. Growth regulators and carbohydrates have been widely studied in tissue culture research. However, macro-elements have also an important effect on tissue culture. Macro-elements play indispensable roles in the rapid propagation of apple dwarfing rootstock. Reasonable dosage and composition of macro-elements are important for rapid propagation system.【Methods】A five-factor quadratic regression orthogonal rotation design (1/2) was used to construct the experiment. Concentrations of macro- elements in MS medium including KNO3, NH4NO3, MgSO4 · 7H2O, KH2PO4 and CaCl2 · 2H2O were set as independent variables. The amounts of trace-element followed the standard MS medium. 6-BA at 1.0 mg · L-1 and IBA at 0.1 mg · L-1 were used as growth regulators, and the pH value was adjusted to 5.8. The temperature of the culture chamber was controlled at (232) ℃; the photoperiod was 16 h/8 h (day/night); the light intensity was 30 µmol · m-2 · s-1. Statistical analysis was carried out after 35 days of culture. The subculture coefficient and the average height of seedlings
养基的大量元素影响苹果矮化砧木‘SH6’的继代系数和幼苗高度，KNO3与 NH4NO3是主要影响因素。大量元素优化 配方为 ：KNO3 1 535 mg · L-1、NH4NO3 1 430 mg · L-1、MgSO4 · 7H2O 310 mg · L-1、KH2PO4 172 mg · L-1 与 CaCl2 · 2H2O 445
摘 要：【目的】探讨培养基大量元素组分和配比对苹果矮化砧木‘SH6’组培继代的影响，为提高组培快繁效率、建立
快繁技术提供理论依据。【方法】采用五因素二次回归正交旋转设计(1/2 实施)方法，以 MS 培养基大量元素 KNO3、
NH4NO3、MgSO4·7H2O、KH2PO4与 CaCl2·2H2O 为自变量，接种后 35 d 的组培苗继代系数和幼苗高度为因变量。【结果】 单因子效应分析表明，大量元素影响苹果矮化砧木‘SH6’继代系数的主次顺序是 KNO3 > NH4NO3 > MgSO4 · 7H2O >
DOI:10.13925/ki.gsxb.20190213
苹果矮化砧木‘SH6 ’组培快繁培养基 大量元素配方的优化
杨雨璋，周贝贝，李民吉，李兴亮，张军科，张 强，魏钦平*
（北京市林业果树科学研究院·农业农村部华北地区研究中 心，北京 100097）
YANG Yuzhang，ZHOU Beibei，LI Minji，LI Xingliang，ZHANG Junke，ZHANG Qiang，WEI Qinping*
(Beijing Academy of Forestry and Pomology Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs/Beijing Engineering Research Center for Deciduous Fruit Trees, Beijing 100097, China)
mg·L-1，培养 35 d 继代系数 4.2，幼苗高度 2.6 cm。
关键词：苹果矮化砧木‘；SH6’；组织培养；大量元素；二次回归正交旋转设计；配方优化
中图分类号：S661.1
文献标志码：A
文章编号：1009-9980（2020）01-0040-10
Optimization of macro- element composition in the medium for in vitro propagation of apple dwarfing rootstock‘SH6’
KH2PO4 > CaCl2 · 2H2O，影响幼苗高度的主次顺序是 KNO3 > NH4NO3 > CaCl2 · 2H2O > MgSO4 · 7H2O > KH2PO4。因子交 互效应表明 ，继代系数受 NH4NO3 与 CaCl2 · 2H2O 交互效应的影响 ；幼苗高度受 KNO3 与 NH4NO3、KNO3 与 KH2PO4、 KNO3与 CaCl2·2H2O 交互效应的影响。配方优化的研究表明，理论预测与实验验证的结果无显著差异。【结论】MS 培
Abstract：【Objective】‘SH6’, a representative Chinese apple dwarfing rootstock, was used in this study to investigate the effect of macro-elements in tissue culture medium on in vitro subculture, in order to improve the efficiency of tissue culture. Growth regulators and carbohydrates have been widely studied in tissue culture research. However, macro-elements have also an important effect on tissue culture. Macro-elements play indispensable roles in the rapid propagation of apple dwarfing rootstock. Reasonable dosage and composition of macro-elements are important for rapid propagation system.【Methods】A five-factor quadratic regression orthogonal rotation design (1/2) was used to construct the experiment. Concentrations of macro- elements in MS medium including KNO3, NH4NO3, MgSO4 · 7H2O, KH2PO4 and CaCl2 · 2H2O were set as independent variables. The amounts of trace-element followed the standard MS medium. 6-BA at 1.0 mg · L-1 and IBA at 0.1 mg · L-1 were used as growth regulators, and the pH value was adjusted to 5.8. The temperature of the culture chamber was controlled at (232) ℃; the photoperiod was 16 h/8 h (day/night); the light intensity was 30 µmol · m-2 · s-1. Statistical analysis was carried out after 35 days of culture. The subculture coefficient and the average height of seedlings
养基的大量元素影响苹果矮化砧木‘SH6’的继代系数和幼苗高度，KNO3与 NH4NO3是主要影响因素。大量元素优化 配方为 ：KNO3 1 535 mg · L-1、NH4NO3 1 430 mg · L-1、MgSO4 · 7H2O 310 mg · L-1、KH2PO4 172 mg · L-1 与 CaCl2 · 2H2O 445
摘 要：【目的】探讨培养基大量元素组分和配比对苹果矮化砧木‘SH6’组培继代的影响，为提高组培快繁效率、建立
快繁技术提供理论依据。【方法】采用五因素二次回归正交旋转设计(1/2 实施)方法，以 MS 培养基大量元素 KNO3、
NH4NO3、MgSO4·7H2O、KH2PO4与 CaCl2·2H2O 为自变量，接种后 35 d 的组培苗继代系数和幼苗高度为因变量。【结果】 单因子效应分析表明，大量元素影响苹果矮化砧木‘SH6’继代系数的主次顺序是 KNO3 > NH4NO3 > MgSO4 · 7H2O >