靶向沉默Notch1基因对胃癌细胞SGC-7901增殖和侵袭的影响

靶向沉默Notch1基因对胃癌细胞SGC-7901增殖和侵袭的
影响
魏光兵;常远鸿;王康;仇广林;柴明明
【期刊名称】《西安交通大学学报（医学版）》
【年(卷),期】2012(033)006
【摘要】Objective To investigate the effect of silencing Notchl gene on cell proliferation in vitro in gastric cancer cell line SGC-7901 and the underlying mechanisms. Methods The siRNA of notchl was transfected into gastric cancer cell line SGC-7901 by Lipofectamine 2000. Proliferation and invasion of SGC-7901 were detected after transfection by methabenzthiazuron assay and transwell assay, respectively. The mRNA expression of Notchl was determined by RT-PCR. The protein expressions of Cyclin Dl, Cyclin Al and CDK2 were determined by Western blotting. The mRNA expressions of MMP-2 and COX-2 were determined by RT-PCR. Results Compared with that in control group, the mRNA expression of Notchl was significantly decreased after transfection. The proliferation and invasion of SGC-7901 cells were inhibited significantly after Notchl silencing. Additionally, the protein expressions of Cyclin Dl and Cyclin Al as well as the mRNA expressions of MMP-2 and COX-2 were obviously inhibited. However, silence targeting Notchl did not affect CDK2. Conclusion RNA silence targeting Notchl can inhibit the proliferation and invasion of gastric cell line SGC-7901 probably through decreasing the
expressions of Cyclin Dl and Cyclin Al and down-regulating the expressions of MMP-2 and COX-2, respectively.%目的探讨靶向沉默Notch1基因对人胃癌细胞SGC-7901增殖和侵袭作用的影响及可能机制.方法通过小干扰RNA转染胃癌细胞SGC-7901,以未转染细胞为正常对照组,转染siRNA Control为阴性对照组,RT-PCR法检测靶向沉默Notch1基因的效果,MTT法检测各组SGC-7901细胞的增殖能力,Transwell小室侵袭试验检测各组SGC-7901细胞的侵袭能
力,Western blot检测细胞周期相关蛋白Cyclin D1、Cyclin A1和CDK2蛋白表达水平,RT-PCR检测MMP-2和COX-2 mRNA的表达水平.结果靶向沉默Notch1基因可明显抑制胃癌SGC-7901细胞增殖,干扰24 h后与正常对照组或阴性对照组比较差异有统计学意义.靶向沉默Notch1基因明显下调细胞周期相关蛋白Cyclin D1和Cyclin A1,但对CDK2无影响.靶向沉默Notch1基因明显抑制胃癌SGC-7901细胞侵袭能力,同时明显下调MMP-2和COX mRNA的水平.结论靶向沉默Notch1基因可抑制胃癌细胞SGC-7901增殖和侵袭,可能与其抑制细胞周期相关蛋白Cyclin D1、Cyclin A1和侵袭相关分子MMP-2和COX-2有关.【总页数】5页(P760-764)
【作者】魏光兵;常远鸿;王康;仇广林;柴明明
【作者单位】西安交通大学医学院第一附属医院普通外科,陕西西安710061;西安第四医院消化内科,陕西西安710004;西安交通大学医学院第一附属医院普通外科,陕西西安710061;西安交通大学医学院第一附属医院普通外科,陕西西安710061;西安交通大学医学院第一附属医院普通外科,陕西西安710061
【正文语种】中文
【中图分类】R735.2
【相关文献】
1.siRNA沉默mdm2基因对胃癌细胞SGC-7901增殖和Rb基因蛋白表达的影响[J], 杨海霞;成诗银;米建强;王亮;杨国栋;徐继业
2.慢病毒介导的αB-晶状体蛋白基因沉默对胃癌细胞SGC-7901增殖和迁移能力的影响 [J], 张峰;赵达;侯小明;袁得峰;张彦军;金延玲;李敏
3.DEK基因在胃癌中的表达及调控Notch1信号通路对胃癌细胞增殖及侵袭的影响 [J], 邢苑;韩钧凌;俞力军;陈和萍;张宏
4.siRNA 靶向沉默PcG蛋白EZH2对人胃癌细胞株 MKN45增殖和侵袭力的影响[J], 蔡干慧;陈晓宇
5.微小RNA-645靶向IFIT2对幽门螺杆菌感染胃癌细胞增殖和侵袭的影响微小RNA-645靶向IFIT2对幽门螺杆菌感染胃癌细胞增殖和侵袭的影响 [J], 陈德薇;张新星
因版权原因，仅展示原文概要，查看原文内容请购买。