Method is used to prepare a next-generation sequen
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专利名称：Method is used to prepare a next-
generation sequencing library (NGS) from
ribonucleic acid (RNA) sample and for
implementing identical composition
发明人：CHANG, Cynthia,BOSTICK, Magnolia
申请号：EP16847330
申请日：20160915
公开号：EP3350732A4
公开日：
20190227
专利内容由知识产权出版社提供
摘要：The method for preparing the next-generation library sequencing (NGS) from a ribonucleic acid (RNA) provides sample. The aspect of method, it includes the RNA samples for cDNA the first chain priming paint and a template switch oligonucleotide that combination has under cDNA the first chain synthetic technological conditions, and one of cDNA the first chain priming paint and template switch oligonucleotide include a first door pillar-tagmentation amplimers bond area here. Resultant product is limited by the amplification condition for being enough to produce a bifilar complementary DNA, followed by the tagmented together with it of a transpo including second column-tagmentation amplimers bond area. Tagmented samples are then subjected to the amplificationconditions used first including alignment problem platform adapter and the second column-tagmentation amplification primers are constructed to produce the library NGS. Aspect of the invention further includes the composition generated by being applied to the Method and kit for of implementation method.
申请人：Takara Bio USA, Inc.
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