miRNA的敲除过表达实验,对照组如何设置

直接转染RNA链和转染质粒，有什么区别？
如果是做miRNA敲除/过表达实验，选择哪种，为什么？
文献似乎两者兼有。

另外，设计miRNA的敲除过表达实验，对照组如何设置？
perfect complementary，anti-miRNA，pre-miRNA，mature miRNA，anti-miRNA-mutation，pre-miRNA-mutation，mature miRNA-mutation，random sequence，空载体，无转染的细胞以上这些，哪些需要包含在实验设计分组中，哪些没有必要呢？
Direct siRNA transfection efficiency will be lower than that of transfection with plasmid DNA, and the efficiency varies markedly among each direct siRNA transfection. If you have miRNA construct, use that construct plasmid DNA for transfection as each RNAi experiment with plasmid transfection can be evaluated by compatible transfections.
In control plasmid, you should use scramble sequences (the length of randomly chosen nucleotides should be quite same as the length of your miRNA.
In what you have cited different forms of miRNA, you can choose random sequences, empty vector or mock transfection cells as negative control.。