兔骨髓间充质干细胞体外培养定向诱导分化为软骨细胞

兔骨髓间充质干细胞体外培养定向诱导分化为软骨细胞
俞猛;于方;付胜良
【期刊名称】《中国组织工程研究》
【年(卷),期】2011(015)027
【摘要】BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have a multi-differentiated potential to differentiate into bone, cartilage, tendon, adipose and so on.OBJECTIVE: To build a method of differentiation of rabbit BMSCs into chondrocytes in vitro and to determine the factors involved in the differentiation process.METHODS: BMSCs were isolated from young rabbit femur marrow, and then planted in plastic culture flask. After stimulated by basic fibroblast growth factor (bFGF), passage 3 cells were chosen to induce into cartilage differentiation in different concentrations of transforming growth factor-?1 (TGF-?1). Tow weeks later, the morphology of chondrocytes was detected by toludine blue, type Ⅱ collagen and aggrecan assay with RT-PCR.RESULTS AND CONCLUSION: bFGF could stimulate the proliferation of BMSCs. Induced BMSCs had typical appearance of chondrocytes, the expression of type Ⅱ collagen and aggrecan were positive. 10 μg/L TGF-?1 was the optimal concentration for cartilage differentiation.%背景:骨髓间充质干细胞具有分化为骨、软骨、肌腱、脂肪等组织的多分化潜能.目的:体外培养兔骨髓间充质干细胞并定向诱导分化为软骨细胞,探讨体外诱导分化为软骨的方法和条件.方法:取兔股骨骨髓,全骨髓贴壁法分离培养骨髓间充质干细胞,碱性成纤维生长因子体外培养后,取第3 代细胞在培
养基中添加不同质量浓度转化生长因子β1 的软骨分化诱导剂,2 周后倒置显微镜观察细胞形态,甲苯胺蓝染色,RT-PCR技术检测Ⅱ型胶原和聚集蛋白聚糖的表达.结果与结论:可以从兔骨髓中培养出骨髓间充质干细胞,碱性成纤维生长因子能明显的促进骨髓间充质干细胞增殖.经软骨诱导分化后骨髓间充质干细胞呈软骨细胞形态,甲苯胺蓝染色阳性,Ⅱ型胶原和聚集蛋白聚糖的表达阳性.10 μg/L 的转化生长因子β1 诱导成软骨分化能力最强.
【总页数】4页(P4951-4954)
【作者】俞猛;于方;付胜良
【作者单位】首都医科大学附属北京世纪坛医院骨科,北京市100038;首都医科大学附属北京世纪坛医院骨科,北京市100038;首都医科大学附属北京世纪坛医院骨科,北京市100038
【正文语种】中文
【中图分类】R394.2
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