A201003-591

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德固赛金属加工液助剂产品列表

1015（消泡剂）
改性硅氧烷为基础的浓缩乳液
2-89（消泡剂）改性硅氧烷乳液
V20
聚氧乙烯醚-20 油酰篦麻醇酸酯
W 5840（润湿剂，改性硅氧烷的润
沉降剂）
湿剂
AC 101(防锈剂)
妥尔油脂肪酸乙氧基化单乙醇胺 (MEA)浓缩液
乳白，不透明白色乳液清澈液体
清澈液体
◇在金属切削液、工业和家庭用清洗剂、纺织配方中作为抑泡剂.
德国赢创德固赛公司金属加工液助剂产品列表
名称
类型
形态
典型应用领域
AL 200（铝镁缓蚀脂肪醇乙氧基化
剂、极压剂）
磷酸酯
透明液体
◇轧制、切削、冲压（微）乳化油
建议用量
0.5%-3%
改性脂肪酸胺的清澈，褪
AC 28（防锈剂）浓缩液.
色液体 ◇金属加工液的抑腐剂和防锈剂，推荐用于乳化油、微乳液
2-5%
0.03%-0.1%
◇多种工业用途,解决基材与涂层间不兼容的不良使用效果。
◇在金属加工油品行业，常被用作腐蚀抑制剂和乳化剂。
◇与磺酸钠、非离子表面活性剂、硼酸胺一起用于金属加工油品。
◇与磺酸钠搭配用于工业清洗时，可增强清洗剂的润湿性能和脱脂能力。
3-5%
◇对铸铁有良好的缓蚀效果，能提高工件的光洁度以及改善金属碎屑的沉降性，避免积屑瘤
◇Tagat V20 具有较强的防腐能力,能有效弥补植物油易腐败的缺陷； ◇Tagat V20 配制的乳液粒径小、低泡；
10-15%
◇TAGAT® V 20 是由植物原材料生产出来的，有良好的皮肤试验报告 (对皮肤无刺激)
◇TEGO®SurtenW5840 可以改善水性体系的铺展及润湿性能。

出口商品代码

0
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ab ab ab ab ab ab abfe ab abfe ab ab ab ab fe ------------
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-----10 91 99 10 90 ---10 90 -----------
香水及花露水唇用化妆品眼用化妆品指(趾)甲化妆品香粉,不论是否压紧护肤品其他含濒危植物成分美容化妆品其他美容化妆品含濒危植物成分的洗发剂其他洗发剂(香波) 烫发剂定型剂其他护发品含濒危植物成分牙膏其他牙膏其他洁齿品清洁牙缝用的纱线(牙线) 其他口腔及牙齿清洁剂剃须用制剂人体除臭剂及止汗剂香浴盐及其他沐浴用制剂神香及其他通过燃烧散发香气制品其他室内除臭制品其他编号未列名的芳香料制品其他初级形状的丙烯酸聚合物
45
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
0
17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17
17
30 30 30 30 30 0 30 30 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
33030000 33041000 33042000 33043000 33049100 33049900 33049900 33049900 33051000 33051000 33052000 33053000 33059000 33061010 33061010 33061090 33062000 33069000 33071000 33072000 33073000 33074100 33074900 33079000

科莱恩重点推荐产品

科莱恩重点推荐产品HLB EPA 组成活性物质含量浊点脂肪醇乙氧基化合物(calc.) (%);外观 (?)ca.80.5 11 910,930 液体乳化剂，增效剂带有8个EO的油醇聚乙二醇醚100;液体糊状 ?Genapol O 080ca.50.0 12 910,930 液体乳化剂，增效剂带有10个EO的油醇聚乙二醇醚100;糊状类似蜡 ?Genapol O 100ca.65.5 11 910,930 液体乳化剂，润湿剂带有6个EO的异13醇聚乙二醇醚100;液体 ?Genapol X 060ca.75.5 13 910,930 液体乳化剂，润湿剂带有8个EO的异13醇聚乙二醇醚100;液体 ?Genapol X 080脂肪醇乙氧基化合物ca.67.0 9 910,930 矿物油乳化剂油醇聚乙二醇醚 ca.100;液体 ?Emulsogen M(低倾点(ca.0?)，在低至ca.12?时仍能保持均一性)三苯乙烯苯酚乙氧基化合物(600#)ca.60.0 13 920 乳化剂带有16个EO的2，4，6-三(1-苯乙基)100;液体 ?Emulsogen TS 160-酚聚乙二醇醚ca.57.5 13.5 920 乳化剂带有20个EO的2，4，6-三(1-苯乙基)100;液体糊状 ?Emulsogen TS 200-酚聚乙二醇醚蓖麻油乙氧基化合物64.0-69.0 13.5 960 乳化剂带有36个EO的蓖麻油乙氧基化合物 100;液体 ?Emulsogen EL360EO/PO 嵌段共聚物ca.56.8 14 960 液体分散剂,乳化剂含有20%EO的EO/PO嵌段共聚物 100;液体 ?Genapol PF 20ca.85.0 11 960 液体分散剂,乳化剂正丁醇烷氧物 100;类似蜡 ?Emulsogen 3510ca.67.8 16 960 液体分散剂,乳化剂含有40%EO的EO/PO嵌段共聚物 100;液体 ?Genapol PF 40ca.40.5 12 920 液体分散剂,乳化剂脂肪醇烷氧化物 100;液体 ?Genapol EP 2584ca.72.9 6 960 液体分散剂,乳化剂 EO/PO嵌段共聚物 100;糊状 ?Emulsogen V1816三丁基苯酚乙氧基化合物ca.67.0 11 液体乳化剂, 润湿剂带有8个EO的三仲丁基酚聚乙二醇醚 100;液体未豁免 ?Sapogenat T 080ca.43.0 13 液体乳化剂, 润湿剂带有11个EO的三仲丁基酚聚乙二醇醚 100;液体未豁免 ?Sapogenat T 110EPA 组成活性物质含量种类水溶性分散剂 (100%);外观固体分散剂甲酚-甲醛钠盐的浓缩产品 ca.80;粉末阴离子溶未豁免 ?Dispersogen 1494SC,WDG,WP 固体分散剂脂肪酸甲基牛磺酸钠盐 ca.60-65;粉末阴离子溶910，?Hostapon TPHCWDG 920，(950)萘磺酸盐分散剂 Coralon OTWP,WDG固体润湿剂WP,WDG Hostapur OSB非离子液体梳状共聚聚丙烯酸酯接枝共聚物 ca.100;糊状非离子溶未豁免 ?Dispersogen PSL 100物 SC阴离子液体梳状共聚Dispersogen ACP 120物 SC磷酸酯酸 Dispersogen LFHSC磷酸酯盐 Dispersogen LFSSC磷酸酯盐 Dispersogen Agent 3618SC钙盐支链钙盐70% Phenylsulfonat CA直链钙盐70% Phenylsulfonat CAL草甘膦助剂牛脂胺 Genamin 267绿色性 Synergen GL 5两性离子 Synergen G2D 溶剂绿色溶剂 Genagen 4166 绿色溶剂 Genagen 4296。

100818 常用塑胶材料牌号

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日本三丰总代理游标卡尺货号大全

日本三丰量具总代理游标卡尺货号大全来源：青岛首丰精密机电有限公司 tel：400-0532-510日本三丰防水防油数显卡尺货号：500-702-10/500-712-10/500-706-11/500-716-11/500-709-11/500-719-10/500-721-10/500-723-10/500-727-11/500-703-10/500-713-10/500-707-11/500-717-11/500-722-10/500-724-10/500-728-11/500-714 -10/500-718-11/500-704-10/500-708-11日本三丰数显卡尺货号：500-150-20；500-180-20；500-151-20；500-154-20；500-155-20；500-158-20；500-181-20；500-152-20；500-156-20;500-157-20;500-182-20;500-153;500-500-10;500-501-10;500-502-10/500-170-20/500-195-20/5 00-171-20/500-174-20/500-175-20/500-178-20/500-196-20/500-159-20/500-160-20/500-172-20/500-176-20/500-177-20/500-197-20/500-163-20/500-164-20/500-173/500-167/500-168/500-193/500-165/500-166/ 500-505-10/500-506-10/500-507-10日本三丰游标卡尺货号：530-102/530-101/530-320/530-335/530-319/530-122/530-108/530-321/530-123/530-109/530-322/530-124/530-501/530-502/530-104/530-316/530-125/530-312/530-114/530-118/530-115/530-119/530-105/530-3 14/530-116日本三丰带圆弧刃量爪卡尺货号：数显类550-301-10/550-331-10/550-203-10/550-205-10/550-207-10/550-311-10/550-341-10/550-223-10/550-225-10/550-227-10；游标类：160-170/160-180/160-130/ 160-131/160-132 日本三丰低测力数显卡尺货号：573-191-20/573-291-20日本三丰快速卡尺货号：573-181-20/573-182-20/573-281-20/573-282-20日本三丰内径卡尺货号：573-642/573-643/573-645/573-647/573-646/573-648/573-742/573-745/573-746/536-142/536-145/536-146/536-147/536-148/536-149日本三丰背置量爪型中心线卡尺货号：573-116-10/573-117-10/573-118-10-573-119-10日本三丰薄片卡尺货号：573-634/573-635/573-734/536-134/536-135/536-136日本三丰长量爪游标卡尺货号：534-109/534-110/534-113/534-114/534-115/534-116/534-101/534-105/534-102/534-106/534-103/534-107/534-104/534-108/534-117/534-118/534-119/534-120日本三丰带表卡尺货号：505-680/505-671/505-683/505-707/505-711/505-681/505-685/505-672/505-684/505-682/505-686/505-67 3/505-674/505-675/505-689/505-708/505-712/505-676/505-690/505-709/505-713/505-720/505-677/505-721/505-710/505-714日本三丰钩式游标卡尺货号：536-171/536-172日本三丰管壁厚度卡尺货号：573-661/573-662/573-761/536-161日本三丰划线卡尺货号：573-676/573-677/573-679/536-221/536-222/536-223日本三丰尖爪卡尺货号：573-621/573-625/573-622/573-626/573-721/573-725/536-121日本三丰偏置卡尺货号：573-601/573-611/573-602/573-612/573-604/573-614/573-701/573-702/573-704/536-101/536-102/536-10 3日本三丰偏置中心线卡尺货号：573-605/573-615/573-606/573-616/573-608/573-618/573-705/573-706/573-708/536-105/536-106/536-107日本三丰塑料卡尺货号：700-127/700-128/700-126日本三丰外凹槽卡尺货号：573-651/573-652/573-653/573-654/573-751/573-752/536-151/536-152日本三丰旋转型游标卡尺货号：536-212日本三丰带有圆弧刃量爪游标卡尺货号：160-130/160-131/160-132/160-133/160-134/160-127/160-128/160-101/160-104/160-110/160-113/160-150/160-151/160-153/160-155/160-157/160-159/160-124/160-116/160-102/160-105/160-11 1/160-114/160-125/160-119/160-103/160-106/160-112/160-115。

商品归类代码说明第九十章一

商品归类代码说明第九十章一商品归类代码说明（第九十章一）商品名称编码附加编码附加序号说明光导纤维 90011000 - 001 但编号8544的货品除外光导纤维束 90011000 - 002 -光导纤维束及光缆 90011000 - 003 但编号8544的货品除外光缆 90011000 - 004 但编号8544的除外光纤 90011000 - 005 但编号8544的除外反光片 90012000 - 001 偏振材料制的反射偏光片 90012000 - 002 偏振材料制的偏光片 90012000 - 003 -偏振材料片 90012000 - 004 -偏振片 90012000 - 005 -偏振元件 90012000 - 006 -抛弃型隐形眼镜片 90013000 - 001 -一次性隐形眼镜 90013000 - 002 -隐形眼镜 90013000 - 003 -隐形眼镜镜片毛坯 90013000 - 004 -玻璃制变色镜片 90014010 - 001 -太阳镜片 90014091 - 001 玻璃制的太阳眼镜玻璃片 90014091 - 002 -玻璃眼镜片 90014099 - 001 非太阳镜片老花镜片 90014099 - 002 玻璃制的非玻璃材料制变色镜片 90015010 - 001 -非玻璃材料制太阳镜片 90015091 - 001 -塑胶太阳镜片 90015091 - 002 -塑料太阳镜片 90015091 - 003 -太阳镜片 90015091 - 004 非玻璃制的亚克力太阳镜片 90015091 - 005 -老花镜片 90015099 - 001 非玻璃制的树脂镜片 90015099 - 002 -非眼镜用的玻璃镜片 90019000 - 001 未经装配的、经光学加工的非眼镜用的镜片 90019000 - 002 未经装配的、经光学加工的光学镜片 90019000 - 003 非眼镜用的、未经装配的棱镜 90019000 - 004 -滤光片 90019000 - 005 -滤色镜片 90019000 - 006 -透镜 90019000 - 007 -相机用镜片 90019000 - 008 未经装配的衍射光栅 90019000 - 009 -特殊用途照相机用物镜 90021110 - 001 -缩微阅读机用物镜 90021120 - 001 -相机用物镜 90021190 90 001 特种照相机和缩微阅读机用除外已装配的透镜 90021190 90 002 特种照相机和缩微阅读机除外照相机物镜镜头 90021190 90 003 特种照相机和缩微阅读机用除外已装配的透镜 90021990 - 001 税号90021110至90021910除外已装配的物镜 90021990 - 002 税号90021110至90021910除外照相机用滤色镜 90022010 - 001 -已装配的滤光镜 90022090 - 001 照相机用的除外防盗专用镜头 90029090 - 001 无电器元件取景器 90029090 - 002 -野生动物产品制眼镜架 90031900 10 001 -眼镜架 90031900 90 001 非塑料制的已电镀眼镜架 90031900 90 002 -鼻垫 90039000 - 001 眼镜架用的鼻粒 90039000 - 002 眼镜架用的鼻梁架 90039000 - 003 眼镜架用的鼻托 90039000 - 004 眼镜架用的鼻须 90039000 - 005 眼镜架用的鼻中 90039000 - 006 眼镜架用的边丝 90039000 - 007 眼镜架用的合口 90039000 - 008 眼镜架用的夹口 90039000 - 009 眼镜架用的金属眼镜臂 90039000 - 010 -铜鼻顶 90039000 - 011 眼镜架用的铜鼻尾 90039000 - 012 眼镜架用的铜脚 90039000 - 013 眼镜架用的托叶 90039000 - 014 眼镜架用的眼镜架用铰链 90039000 - 015 -太阳镜 90041000 - 001 -太阳眼镜 90041000 - 002 -安全眼镜 90049090 - 001 -单片眼镜 90049090 - 002 -挡风镜 90049090 - 003 -防护镜 90049090 - 004 -护目镜 90049090 - 005 -偏光眼镜 90049090 - 006 -双筒望远镜 90051000 - 001 -望远镜 90051000 - 002 双筒的.赤道仪 90058010 - 001 -地平经度度盘 90058010 - 002 -地平经纬仪 90058010 - 003 -定天镜 90058010 - 004 -反射望远镜 90058010 - 005 -量日仪 90058010 - 006 -日光观测镜 90058010 - 007 -日光摄谱仪 90058010 - 008 -天顶仪 90058010 - 009 -天文折射望远镜 90058010 - 010 -中星仪 90058010 - 011 -单筒望远镜 90058090 - 001 -望远镜右内筒 90059090 - 001 -望远镜右外壳 90059090 - 002 -望远镜左内筒 90059090 - 003 -望远镜左外壳 90059090 - 004 -电子分色机 90061010 - 001 -制版照相机 90061090 - 001 -缩微照相机 90062000 - 001 -比较照相机 90063000 - 001 法庭或犯罪学用激光照相仪 90063000 - 002 -激光照相仪 90063000 - 003 特种用途用特种照相机 90063000 - 004 -一次成像照相机 90064000 - 001 -单镜头反光式照相机 90065100 - 001 -暗箱式照相机 90065900 - 001 -记录照相机 90065900 - 002 -立体照相机 90065900 - 003 -全景照相机 90065900 - 004 -袖珍照相机 90065900 - 005 -照相机 90065900 - 006 税号90061010至90065300除外折叠式照相机 90065900 - 007 -方形闪光灯 90066100 - 001 -闪光灯 90066100 - 002 -闪光灯组件 90066100 - 003 放电式闪光灯管 90066200 - 001 非放电式照相用闪光灯泡 90066200 - 002 -闪光管 90066200 - 003 非放电式照相用自动对焦组件 90069191 - 001 -快门组 90069192 - 001 不含90069110,9120商品快门组件 90069192 - 002 不含90069110,9120商品按钮 90069199 - 001 照相机用窗口 90069199 - 002 照相机用电池盖 90069199 - 003 照相机用光圈 90069199 - 004 -球窝式云台 90069199 - 005 照相机用取景窗 90069199 - 006 -照相机背盖 90069199 - 007 -照相机本体 90069199 - 008 未构成整机照相机部件单品类 90069199 - 009 除其它税号列名照相机机身 90069199 - 010 -照相机外壳 90069199 - 011 -照相机线轴 90069199 - 012 -照相机压板 90069199 - 013 -照相机专用齿轮 90069199 - 014 -。

除油粉专用表面活性剂

进口
C-201
无泡活性剂
（聚乙烯醇丙烯醚）
200KG
适用强力喷淋高碱无泡除油粉配置，几乎无泡沫
进口
C—300
除油除蜡活性剂
200KG
适合配置钢铁合金等高低碱度除油除蜡粉，不上基材，清洗速度奇快！按推介配方配置效果更佳
本厂
渗透剂TA-2
电解脱脂活性剂
(乙基丙醇聚氧乙烯醚)
200KG
适合配置钢铁合金电解除油粉耐高温高碱，适合阴阳极电解，泡沫低不爆炸
进口
AL-602
聚醚多元醇602
200KG
适用强力喷淋高碱无泡除油粉配置，几乎无泡沫，也可用于消泡剂使用，主要是内置消泡剂，不同于一般的消泡剂
中国
NNF
扩散剂
25KG
黑碱除油王主料
络合粉
25KG
电解脱脂粉用
DL-211
氨基酸盐
50KG
玻璃不锈钢清洗
除油粉系列表面活性剂
产地
产品编号
产品名称
包装规格
PH
简要说明
进口
C-202
中常温活性剂
（聚醚合成）
215KG
中常温除油粉配置专用，分解快，无浮油，耐酸碱！
进口
C-202A
中Hale Waihona Puke 温活性剂200KG中高温除油粉配置专用，耐强碱，对清洗拉伸油重油特别突出
进口
C-200
低泡活性剂
(乙酯乙烯醚)
200KG
用于配置低泡除油粉，耐酸碱，高温，适合喷淋清洗剂！

日本白光HAKKO主机型号大全

锡线 HEXSOL FS301-01 FS302-01 FS302-02 FS302-03 FS302-04 FS303-01 FS303-02
锡线 HEXSOL FS300-01 FS400-01 FS400-02 FS401-01 FS401-02
锡线 HEXSOL FS402-01 FS402-02 FS402-03 FS402-04
化学助焊膏 PASTE(FS-120) FS120-01 FS120-02
助焊剂 001 001-01 001-02 001-03 001-04
助焊剂 CHEMICAL 1
助焊剂 SUSSOL-F 89-031 89-100 89-400 89
锡线 HEXSOL FS500-01 FS501-01 FS501-02 FS501-03 FS600-01 FS600-02 FS600-03 FS600-04
化学膏 FS-100 FS-100
焊咀清洁器 FT-700 FT700-01
电热剥线钳 FT-801 FT801-01
切割机电动装备 152B 152B
电阻切割成形机 153 153-1
电阻切割成形机 154 154-1
电阻切割机 155 155-1 155-2
精钢电子工具剪钳 106-01~08 106-01 106-02 106-03 106-04 106-05 106-06 106-07 106-08
自动出锡焊铁 958 958-01 958-02
自动出锡焊铁 959 959-01 959-02
电烙铁 FX-600 FX600-01 FX600-02
电烙铁 FX-601 FX601-01 FX601-03
定温烙铁 DASH N452 N453 N454 N452E N453E N454E

Zud Heavy Duty Cleaner 产品说明书

SAFETY DATA SHEET1. IdentificationZud Heavy Duty Cleaner Product identifierOther means of identification5409Product Code Cleaning Recommended use None known.Recommended restrictionsManufacturer/Importer/Supplier/Distributor information Manufacturer Malco Products, Inc.Address361 Fairview Ave Company name Contact personWebsite TelephonePhone 800-253-2526Fax330-777-8317Emergency phone numberPhone1-800-424-9300Technical Department E-mailUnited States Barberton, OH 442032. Hazard(s) identificationNot classified.Physical hazards Category 2Skin corrosion/irritationHealth hazards Category 2ASerious eye damage/eye irritation Not classified.Environmental hazards Not classified.OSHA defined hazardsLabel elementsSignal word WarningHazard statement Causes skin irritation. Causes serious eye irritation.Precautionary statementPreventionWash thoroughly after handling. Wear eye protection/face protection. Wear protective gloves.ResponseIf swallowed: Rinse mouth. Do NOT induce vomiting. If on skin: Wash with plenty of water. If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If exposed or concerned: Get medical advice/attention. If skin irritation occurs: Get medical advice/attention. If eye irritation persists: Get medical advice/attention. Take off contaminated clothing and wash before reuse.Storage Store away from incompatible materials.DisposalDispose of waste and residues in accordance with local authority requirements.Hazard(s) not otherwise classified (HNOC)None known.Supplemental information98.63% of the mixture consists of component(s) of unknown acute hazards to the aquaticenvironment. 98.63% of the mixture consists of component(s) of unknown long-term hazards to the aquatic environment.3. Composition/information on ingredientsMixturesCAS number %Chemical nameCommon name and synonyms 13397-24-5Calcium Sulfate Dihydrate(Gypsum)60 - < 701317-65-3Limestone5 - < 106153-56-6Oxalic Acid Dihydrate(Ethanedioc acid, dihydrate) 5 - < 101305-62-0Calcium Hydroxide1 - < 310 - < 20Other components below reportable levels546-93-0Magnesium Carbonate 1 - < 3*Designates that a specific chemical identity and/or percentage of composition has been withheld as a trade secret.4. First-aid measuresMove to fresh air. Call a physician if symptoms develop or persist.Inhalation Remove contaminated clothing. Wash with plenty of soap and water. If skin irritation occurs: Get medical advice/attention. Wash contaminated clothing before reuse.Skin contact Immediately flush eyes with plenty of water for at least 15 minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Get medical attention if irritation develops and persists.Eye contact Rinse mouth. Get medical attention if symptoms occur.IngestionSevere eye irritation. Symptoms may include stinging, tearing, redness, swelling, and blurred vision. Coughing. Skin irritation. May cause redness and pain.Most importantsymptoms/effects, acute and delayedProvide general supportive measures and treat symptomatically. Keep victim under observation.Symptoms may be delayed.Indication of immediatemedical attention and special treatment needed Ensure that medical personnel are aware of the material(s) involved, and take precautions to protect themselves.General information5. Fire-fighting measuresWater fog. Foam. Dry chemical powder. Carbon dioxide (CO2).Suitable extinguishing media Do not use water jet as an extinguisher, as this will spread the fire.Unsuitable extinguishing mediaDuring fire, gases hazardous to health may be formed.Specific hazards arising from the chemicalSelf-contained breathing apparatus and full protective clothing must be worn in case of fire.Special protective equipment and precautions for firefighters Move containers from fire area if you can do so without risk.Fire fightingequipment/instructions Use standard firefighting procedures and consider the hazards of other involved materials.Specific methods No unusual fire or explosion hazards noted.General fire hazards6. Accidental release measuresKeep unnecessary personnel away. Keep people away from and upwind of spill/leak. Wearappropriate protective equipment and clothing during clean-up. Do not touch damaged containers or spilled material unless wearing appropriate protective clothing. Ensure adequate ventilation.Local authorities should be advised if significant spillages cannot be contained. For personal protection, see section 8 of the SDS.Personal precautions,protective equipment and emergency proceduresShould not be released into the environment.Large Spills: Stop the flow of material, if this is without risk. Dike the spilled material, where this is possible. Absorb in vermiculite, dry sand or earth and place into containers. Following product recovery, flush area with water.Small Spills: Wipe up with absorbent material (e.g. cloth, fleece). Clean surface thoroughly to remove residual contamination.Never return spills to original containers for re-use. For waste disposal, see section 13 of the SDS.Methods and materials for containment and cleaning upPrevent further leakage or spillage if safe to do so. Do not contaminate water. Avoid discharge into drains, water courses or onto the ground.Environmental precautions7. Handling and storageAvoid contact with eyes, skin, and clothing. Avoid prolonged exposure. Provide adequateventilation. Wear appropriate personal protective equipment. Observe good industrial hygiene practices.Precautions for safe handlingStore in original tightly closed container. Store away from incompatible materials (see Section 10of the SDS).Conditions for safe storage,including any incompatibilities8. Exposure controls/personal protectionOccupational exposure limitsThe following constituents are the only constituents of the product which have a PEL, TLV or other recommended exposure limit.At this time, the other constituents have no known exposure limits.US. OSHA Table Z-1 Limits for Air Contaminants (29 CFR 1910.1000)Value Components FormType PEL5 mg/m3Respirable fraction.Calcium Hydroxide (CAS 1305-62-0)15 mg/m3Total dust.PEL5 mg/m3Respirable fraction.Calcium SulfateDihydrate(Gypsum) (CAS 13397-24-5)15 mg/m3Total dust.PEL 5 mg/m3Respirable fraction.Limestone (CAS 1317-65-3)15 mg/m3Total dust.PEL5 mg/m3Respirable fraction.Magnesium Carbonate (CAS 546-93-0)15 mg/m3Total . ACGIH Threshold Limit Values Value ComponentsFormType TWA 5 mg/m3Calcium Hydroxide (CAS 1305-62-0)TWA 10 mg/m3Inhalable fraction.Calcium SulfateDihydrate(Gypsum) (CAS 13397-24-5)STEL 2 mg/m3Oxalic AcidDihydrate(Ethanedioc acid,dihydrate) (CAS 6153-56-6)TWA1 mg/m3US. NIOSH: Pocket Guide to Chemical Hazards Value Components FormType TWA 5 mg/m3Calcium Hydroxide (CAS 1305-62-0)TWA5 mg/m3Respirable.Calcium SulfateDihydrate(Gypsum) (CAS 13397-24-5)10 mg/m3TotalTWA 5 mg/m3Respirable.Limestone (CAS 1317-65-3)10 mg/m3TotalTWA5 mg/m3Respirable.Magnesium Carbonate (CAS 546-93-0)10 mg/m3Total No biological exposure limits noted for the ingredient(s).Biological limit values Good general ventilation (typically 10 air changes per hour) should be used. Ventilation rates should be matched to conditions. If applicable, use process enclosures, local exhaust ventilation,or other engineering controls to maintain airborne levels below recommended exposure limits. If exposure limits have not been established, maintain airborne levels to an acceptable level. Provide eyewash station. Eye wash fountain and emergency showers are recommended.Appropriate engineering controlsIndividual protection measures, such as personal protective equipmentFace shield is recommended. Wear safety glasses with side shields (or goggles).Eye/face protectionSkin protectionWear appropriate chemical resistant gloves.Hand protectionWear appropriate chemical resistant clothing. Use of an impervious apron is recommended.OtherIn case of insufficient ventilation, wear suitable respiratory equipment.Respiratory protection Wear appropriate thermal protective clothing, when necessary.Thermal hazards Always observe good personal hygiene measures, such as washing after handling the material and before eating, drinking, and/or smoking. Routinely wash work clothing and protective equipment to remove contaminants.General hygiene considerations9. Physical and chemical propertiesAppearanceSolid.Physical state Powder.Form White Color None.OdorOdor threshold Not available.pH1.5 -2.5 3% in Water Melting point/freezing point Not available.Initial boiling point and boiling range Not available.Flash point Not available.Evaporation rate Not available.Not available.Flammability (solid, gas)Upper/lower flammability or explosive limits Flammability limit - lower(%)Not available.Flammability limit - upper (%)Not available.Explosive limit - lower (%)Not available.Explosive limit - upper (%)Not available.Vapor pressure Not available.Vapor density Not available.Relative density Not available.Solubility(ies)Solubility (water)Not available.Partition coefficient (n-octanol/water)Not available.Auto-ignition temperature Not available.Decomposition temperature Not available.ViscosityNot available.Other informationNot explosive.Explosive propertiesNot oxidizing.Oxidizing properties VOC0 % w/w By Weight10. Stability and reactivityReacts violently with strong alkaline substances. This product may react with reducing agents.Reactivity Material is stable under normal conditions.Chemical stability No dangerous reaction known under conditions of normal use.Possibility of hazardous reactionsContact with incompatible materials. Do not mix with other chemicals.Conditions to avoid Acids. Bases. Reducing agents. Aluminum. Fluorine.Incompatible materials No hazardous decomposition products are known.Hazardous decomposition products11. Toxicological informationInformation on likely routes of exposureInhalationProlonged inhalation may be harmful.Skin contact Causes skin irritation.Eye contact Causes serious eye irritation.IngestionExpected to be a low ingestion hazard.Symptoms related to thephysical, chemical andtoxicological characteristics Severe eye irritation. Symptoms may include stinging, tearing, redness, swelling, and blurred vision. Coughing. Skin irritation. May cause redness and pain.Information on toxicological effectsAcute toxicityNot available.Causes skin irritation.Skin corrosion/irritation Causes serious eye irritation.Serious eye damage/eye irritationRespiratory or skin sensitizationRespiratory sensitizationNot a respiratory sensitizer.This product is not expected to cause skin sensitization.Skin sensitization No data available to indicate product or any components present at greater than 0.1% are mutagenic or genotoxic.Germ cell mutagenicity CarcinogenicityNot classifiable as to carcinogenicity to humans.IARC Monographs. Overall Evaluation of CarcinogenicityNot listed.OSHA Specifically Regulated Substances (29 CFR 1910.1001-1050)Not regulated.US. National Toxicology Program (NTP) Report on CarcinogensNot listed.This product is not expected to cause reproductive or developmental effects.Reproductive toxicitySpecific target organ toxicity -single exposureNot classified.Specific target organ toxicity -repeated exposure Not classified.Aspiration hazard Not an aspiration hazard.Chronic effectsProlonged inhalation may be harmful.12. Ecological informationBecause of the low pH of this product, it would be expected to produce significant ecotoxicity upon exposure to aquatic organisms and aquatic systems.EcotoxicityComponentsTest ResultsSpecies* Estimates for product may be based on additional component data not shown.Calcium Hydroxide (CAS 1305-62-0)AquaticLC50Fish33.8844 mg/l, 96 hoursZambezi barbel (Clarias gariepinus)No data is available on the degradability of this product.Persistence and degradability No data available.Bioaccumulative potential No data available.Mobility in soil Other adverse effectsNo other adverse environmental effects (e.g. ozone depletion, photochemical ozone creation potential, endocrine disruption, global warming potential) are expected from this component.13. Disposal considerationsCollect and reclaim or dispose in sealed containers at licensed waste disposal site. Do not allow this material to drain into sewers/water supplies. Dispose of contents/container in accordance with local/regional/national/international regulations.Disposal instructionsDispose in accordance with all applicable regulations.Local disposal regulationsThe waste code should be assigned in discussion between the user, the producer and the waste disposal company.Hazardous waste code Dispose of in accordance with local regulations. Empty containers or liners may retain some product residues. This material and its container must be disposed of in a safe manner (see:Disposal instructions).Waste from residues / unused productsSince emptied containers may retain product residue, follow label warnings even after container is emptied. Empty containers should be taken to an approved waste handling site for recycling or disposal.Contaminated packaging14. Transport informationDOTNot regulated as dangerous goods.IATANot regulated as dangerous goods.IMDGNot regulated as dangerous goods.Not applicable.Transport in bulk according toAnnex II of MARPOL 73/78 and the IBC Code15. Regulatory informationThis product is a "Hazardous Chemical" as defined by the OSHA Hazard Communication Standard, 29 CFR 1910.1200.US federal regulationsTSCA Section 12(b) Export Notification (40 CFR 707, Subpt. D)Not regulated.CERCLA Hazardous Substance List (40 CFR 302.4)Not listed.SARA 304 Emergency release notificationNot regulated.OSHA Specifically Regulated Substances (29 CFR 1910.1001-1050)Not regulated.Superfund Amendments and Reauthorization Act of 1986 (SARA)Immediate Hazard - Yes Delayed Hazard - Yes Fire Hazard - NoPressure Hazard - No Reactivity Hazard - NoHazard categoriesSARA 302 Extremely hazardous substanceNot listed.NoSARA 311/312 Hazardous chemicalSARA 313 (TRI reporting)Not regulated.Other federal regulationsClean Air Act (CAA) Section 112 Hazardous Air Pollutants (HAPs) ListNot regulated.Clean Air Act (CAA) Section 112(r) Accidental Release Prevention (40 CFR 68.130)Not regulated.Not regulated.Safe Drinking Water Act(SDWA)California Safe Drinking Water and Toxic Enforcement Act of 1986 (Proposition 65): This material is not known to contain any chemicals currently listed as carcinogens or reproductive toxins.US state regulations International InventoriesCountry(s) or region Inventory nameOn inventory (yes/no)*NoAustralia Australian Inventory of Chemical Substances (AICS)No Canada Domestic Substances List (DSL)NoCanadaNon-Domestic Substances List (NDSL)Country(s) or region Inventory name On inventory (yes/no)* China Inventory of Existing Chemical Substances in China (IECSC)Yes Europe European Inventory of Existing Commercial ChemicalNoSubstances (EINECS)Europe European List of Notified Chemical Substances (ELINCS)No Japan Inventory of Existing and New Chemical Substances (ENCS)No Korea Existing Chemicals List (ECL)No New Zealand New Zealand InventoryYes Philippines Philippine Inventory of Chemicals and Chemical SubstancesNo(PICCS)United States & Puerto Rico Toxic Substances Control Act (TSCA) InventoryNo *A "Yes" indicates that all components of this product comply with the inventory requirements administered by the governing country(s)A "No" indicates that one or more components of the product are not listed or exempt from listing on the inventory administered by the governingcountry(s).16. Other information, including date of preparation or last revision08-03-2017Issue date08-03-2017Revision dateVersion #04Malco Products, Inc. cannot anticipate all conditions under which this information and its product, Disclaimeror the products of other manufacturers in combination with its product, may be used. It is theuser’s responsibility to ensure safe conditions for handling, storage and disposal of the product,and to assume liability for loss, injury, damage or expense due to improper use. The informationprovided in this Safety Data Sheet is correct to the best of our knowledge, information and belief atthe date of its publication. The information given is designed only as a guidance for safe handling,use, processing, storage, transportation, disposal and release and is not to be considered awarranty or quality specification. The information relates only to the specific material designatedand may not be valid for such material used in combination with any other materials or in anyprocess, unless specified in the text.This document has undergone significant changes and should be reviewed in its entirety. Revision information。

东洋纺织株式会社试剂说明书

manual THUNDERBIRD Probe qPCR Mix 0910 A4250K THUNDERBIRD™ Probe qPCR MixQPS-101T 1 mL x 1QPS-101 1.67 mL x 3Store at -20°C, protected from lightContents[1] Introduction[2] Components[3] Primer/Probe design[4] Template DNA[5] Protocol1. Standard reaction set up2. Cycling conditions2-1. Real-time PCR conditions using Applied Biosystems 7900HT2-2. Real-time PCR conditions using Roche LightCycler 1.1[6] Related ProtocolcDNA synthesis[7] Troubleshooting[8] Related productsC AUTIONAll reagents in this kit are intended for research purposes only. Do not use for diagnosis or clinical purposes. Please observe general laboratory precautions and observe safety procedures while using this kit.-LightCycler™ is a trademark of Idaho Technology, Inc. and Roche Molecular Systems, Inc.-TaqMan® is a registered trademark of Roche Molecular Systems, Inc.-SYBR® is a registered trademark of Roche Molecular Systems, Inc.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio 1JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************1[ 1 ] Introduction [ 2 ] Components DescriptionTHUNDERBIRD™ Probe qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan® probes. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments.This product is an improved version of Realtime PCR Master Mix (Code No. QPK-101). In particular, reaction specificity and PCR efficiency is enhanced.Features-High specificityThe specificity for the detection of low-copy targets is improved.-Homogeneous amplificationThe dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending)-Broad dynamic rangeHigh specificity and effective amplification enable the detection of a broad dynamic range.-Compatibility for various real-time cyclers.The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the 50x ROX reference dye is individually supplied with this kit, the kit can be applied to real-time cyclers that require a passive reference dye.-Hot start PCRThe master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.About the fluorescent probe detection systemThe TaqMan® probe system utilizes fluorescence emission from the probes. The probes hybridize to the target amplicons and then emit fluorescence upon degradation by the 5'-3' exonuclease activity of Taq DNA polymerase. This type of detection system can achieve higher specificity in real-time PCR assays than the SYBR® Green I detection system.This kit includes the following components for 40 reactions (QPS-101T) and 200 reactions (QPS-101), with 50 μl per reaction. All reagents should be stored at -20°C.<QPS-101T>THUNDERBIRD™ Probe qPCR Mix 1 ml x 150x ROX reference dye 50 μl x 1JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************2[ 3 ] Primer/Probedesign <QPS-101>THUNDERBIRD™ Probe qPCR Mix 1.67 ml x 350x ROX reference dye 250 μl x 1Notes:-THUNDERBIRD™ Probe qPCR Mix can be stored, protected from light, at 2-8°C for up to 3 months. For longer storage, this reagent should be kept at -20°C and protected from light. No negative effect was detected by 10 freeze-thaw cycles of THUNDERBRID™ Probe qPCR Mix. This reagent does not contain the ROX reference dye.-50x ROX reference dye can be stored, protected from light, at 2-8°C or -20°C. For real-time cyclers that require a passive reference dye, this reagent must be added to the reaction mixture at a concentration of 1x or 0.1x. The master mix solution with the ROX reference dye can be stored, protected from light, at 2-8°C for up to 3 months. For longer storage, this reagent should be kept at -20°C and protected from light. The pre-mixed reagents can be prepared according to the following ratios. [5] Table 1 shows the optimal concentration of the ROX dye.1x solutionTHUNDERBIRD™ Probe qPCR Mix : 50x ROX reference dye = 1.67 ml : 66.8 μl THUNDERBIRD™ Probe qPCR Mix : 50x ROX reference dye = 1 ml : 40 μl0.1x solutionTHUNDERBIRD™ Probe qPCR Mix : 50x ROX reference dye = 1.67 ml : 6.7 μl THUNDERBIRD™ Probe qPCR Mix : 50x ROX reference dye = 1 ml : 4 μlFor real-time cyclers that do not require a passive reference dye, THUNDERBIRD™ Probe qPCR Mix without the ROX reference dye can be used.1. Primer conditionsHighly sensitive and quantitative data depend on primer design. The primer should be designed according to the following suggestions;-Primer length: 20-30 mer-GC content of primer: 40-60%-Target length: ≤ 200 bp (optimally, 80-150 bp)-Melting temperature (Tm) of primers: 60-65°C-Purification grade of primers: Cartridge (OPC) grade or HPLC gradeNotes:-Longer targets (>200 bp) reduce efficiency and specificity of amplification.-Tm of the primers can be flexible, because the Tm value depends on the calculation formula.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************3[ 4 ] Template DNA 2. Fluorescent probeThe probes should be designed according to the guidelines of each probe system. Because insufficiently purified probes may inhibit the reaction, HPLC-grade probes should be used.The following DNA samples can be used as templates.1. cDNANon-purified cDNA, generated by reverse transcription reactions, can be used directly for real-time PCR using THUNDERBIRD™ Probe qPCR Mix. Up to 10% of the volume of a cDNA solution can be used for a real-time PCR reaction. However, excess volume of the cDNA may inhibit the PCR. Up to 20% (v/v) of the cDNA solution from ReverTra Ace® qPCR RT Kit (Code No. FSQ-101) can be used for real-time PCR (see [6]).2. Genomic DNA, Viral RNAGenomic DNA and viral RNA can be used at up to 200 ng in 50 μl reactions.3. Plasmid DNAAlthough super-coiled plasmids can be used, linearized plasmid DNA produces more accurate assays. The copy number of the plasmid DNA can be calculated by the following formula.Copy number of 1μg of plasmid DNA = 9.1 x 1011 / Size of plasmid DNA (kb)JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************4[ 5 ] Protocol1. Reaction mixture setupReaction volume FinalReagent50µl20µlConcentrationDWXµlXµlTHUNDERBIRD™ Probe qPCR Mix 25 µl 10 μl 1xForward Primer 15 pmol 6 pmol 0.3 μM*1Reverse Primer 15 pmol 6 pmol 0.3 μM*1TaqMan® Probe 10 pmol 4 pmol 0.2 μM*150x ROX reference dye 1μl / 0.1 μl 0.4μl / 0.04μl 1x / 0.1x*2DNA solution Y µl Y µlTotal50µl20µlNotes:*1 Primer / probe concentration should be determined according to the manufacturer’sinstructions.Higher primer concentration tends to improve the amplification efficiency, and lowerprimer concentration tends to reduce the non-specific amplification. The primerconcentration should be set between 0.2-0.6 μM.*2 50x ROX reference dye must be added when using real-time cyclers that require apassive reference dye, according to Table 1. Table 1 shows the optimum concentrationof the ROX reference dye. This dye is not necessary for real-time cyclers that do notrequire a passive reference dye.Table 1 Recommended ROX dye concentrationReal-time cycler Optimal dye concentration(dilution ratio)Applied Biosystems 7000, 7300, 7700, 7900HT etc. 1x (50:1)Applied Biosystems 7500, 7500Fast,Stratagene cyclers (Optional) etc.0.1x (500:1)Roche’ cyclers, Bio-Rad cyclers, BioFlux cyclers etc. Not requiredNotes:The ROX dye in Realtime PCR Master Mix (Code No. QPK-101) corresponds to 1xconcentration.JAPAN CHINA TOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140 www.toyobo.co.jp/e/bio********************52. PCR cycling conditionsThe following table shows the recommended thermal conditions using primers designed according to the recommended primer and probe conditions described in [3]. Almost all targets can also be amplified using the ongoing conditions with other real-time PCR reagents.*1Due to the anti-Taq antibody hot start PCR system, the pre-denaturation can be completed within 60 sec. The pre-denaturation time should be determined according to the recommendations of each real-time cycler. If the optimal pre-denaturation time cannot be determined, the time should be set at 60 sec.Table 2 The recommended pre-denaturation time for each real-time cycler Real-time cycler Pre-denaturation time High speed cycler (e.g. Applied Biosystems 7500Fast) 20 sec Capillary cycler (e.g. Roche LightCycler™ 1.x, 2.0) 30 secGeneral real-time cyclers (Applied Biosystems 7700, 7500,7900HT (normal block), Stratagene cyclers, BioFlux cyclers 60 sec *2The following table shows the optimal denaturation times for each real-time cycler. If the optimal denaturation time cannot be determined, the time should be set at 15 sec.Table 3 The recommended denaturation time for each real-time cycler Real-time cycler denaturation time High speed cycler (e.g. Applied Biosystems 7500Fast) 3 sec Capillary cycler (e.g. Roche LightCycler™ 1.x, 2.0) 5 secGeneral real-time cyclers (Applied Biosystems 7700, 7500,7900HT (normal block), Stratagene cyclers, BioFlux cyclers 15 sec *3Insufficient amplification may be improved by decreasing the extension temperature, and non-specific amplification (e.g. abnormal shapes of the amplification curve at low template concentrations) may be reduced by increasing the extension temperature. The extension temperature should be set at 56-64°C. *4If the target size is smaller than 300 bp, the extension time can be set at 30 sec on almost all real-time cyclers. Instability of the amplification curve or variation of data from each well may be improved by setting the extension time at 45-60 sec. Some real-time cyclers or software need over 30 sec for the extension step. In these cases, the time should be set according to each instruction manual (e.g. Applied Biosystems 7000/73000: ≥ 31 sec; Applied Biosystems 7500: ≥ 35 sec.).<3-step cycle> Temperature Time Ramp Pre-denaturation:95°C 20-60 sec *1Maximum Denaturation:95°C 1-15 sec *2 MaximumExtension: 60°C *3 30-60 sec *4 Maximum (data collection should be set at the extension step)JAPAN CHINA TOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140 www.toyobo.co.jp/e/bio********************62-1. Real-time PCR conditions using Applied Biosystems 7900HT(Normal block type, software version 2.2.2)The following is an example of a TaqMan ® assay using Applied Biosystems 7900HT.(1) The cycling parameters should be set according to the following “Thermal CyclerProtocol” window under the “Instrument” tab.Notes:- Appropriate sample volumes should be set. - ≥ 45 sec is necessary for the extension step.(2) Click the “Data collection” tab.(3) Insert the PCR plate(4) Start the programJAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************72-2. Real-time PCR conditions using Roche LightCycler 1.1(Software version 3.5)The following is an example of a TaqMan® probe assay using Roche LightCycler 1.1. (1)The cycling parameters should be set according to the following window. Analysisand Acquisition mode of the initial denaturation step must be set at “None”. (2)The cycling parameters should be set according to the following window. Analysismode of the PCR step must be set at “Quantification”. Acquisition modes of 95°C and 60°C must be set at “None” and “Single”, respectively.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************8(3)The cycling parameters should be set according to the following window. Analysisand Acquisition mode of the cooling step must be set at “Non”.(4) Insert the capillaries in the carousel, and start the cycling program.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************9[ 6 ] Related Protocol1. cDNA synthesiscDNA synthesized by various cDNA synthesis reagents can be used withTHUNDERBIRD™ Probe qPCR Mix. However, cDNA synthesized by a reagentspecialized for real-time PCR can increase sensitivity.ReverTra Ace® qPCR RT Kit (Code No. FSQ-101) is a cDNA synthesis kit suitable forreal-time PCR. Here, the protocol with ReverTra Ace® qPCR RT Kit is described.However, for the detailed protocol, please refer to the instruction manual of the kit.(1) Denaturation of RNAIncubate the RNA solution at 65°C for 5 min and then chill on ice.Notes:-This step can be omitted. But this step may increase the efficiency of the reversetranscription of RNA, which forms secondary structures.-Do not add 5x RT Buffer and/or enzyme solution at this step.(2) Preparation of the reaction solutionReagent V olume (amount)Nuclease-freeWaterXµl5x RT Buffer 2 µlPrimerMix 0.5µlEnzymeMix 0.5µlRNAsolution 0.5pg-1 µgTotal10µl(3) Reverse transcription reaction-Incubate at 37°C for 15 min. <Reverse transcription>-Heat at 98°C for 2 min. <Inactivation of the reverse transcriptase>-Store at 4°C or -20°C.**This solution can be used in the real-time PCR reaction directly or after dilution.Notes:The above temperature conditions are optimized for ReverTra Ace® qPCR RT Kit.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************10[ 7 ] TroubleshootingSymptom Cause SolutionLoss of linearity in the high cDNA/DNA concentration region. Inhibition by the components in thecDNA/DNA solution.-DNA: The DNA sample may contain PCRinhibitors. The DNA samples should berepurified.-cDNA: The components in the cDNA synthesisreagent may inhibit the PCR reaction. The cDNAsample should be used after dilution.The template DNA is insufficient. When the DNA/cDNA copy number is lower than10 copies per reaction, the linearity of the reactiontends to be lost. The template concentrationshould be increased.Adsorption of the DNA to the tubewall.The diluted DNA templates tend to be absorbedonto the tube wall. Dilution should be performedjust prior to experiments.Lost of linearity or lower signal in the lowDNA/cDNAconcentration region. Competition with primer dimerformation. In the probe assay, primer dimers are not detected. However, dimer formation may reduce the amplification efficiency of the target, especially for reactions at low template concentration. The reaction conditions should be optimized or the primer sequences should be changed.Loss of linearity of the amplification carves. Competition with non-specificamplification.In the probe assay, non-specific amplification isnot detected. However, non-specific amplificationmay reduce the amplification efficiency of thetarget. The reaction conditions should beoptimized or the primer sequences should bechanged.Inappropriate cycling conditions. Optimize the cycling conditions according to [5]. Degradation of the primers. Fresh primer solution should be prepared.The PCR efficiency is lower than 90% (slope:<-3.6) The calculation of the PCRefficiency is inappropriate. The Ct value on the linear region should be used to calculate PCR efficiency.The PCR efficiency is higher than 110%. The calculation of the PCRefficiency is inappropriate.The Ct value on the linear region should be usedto calculate PCR efficiency.Poor purification of the templateDNA.Low-purity DNA may contain PCR inhibitors.Re-purify the DNA samples.Absorption of the template DNA tothe tube wall.Diluted DNA templates tend to be absorbed ontothe tube wall. Dilution of the templateDNA/cDNA should be performed just prior toexperiments.Plasmid DNA or PCR product isused as a template.In general, plasmid DNA or PCR product is usedat low concentration. Diluted DNA templates tendto be absorbed onto the tube wall. Dilution of thetemplate DNA/cDNA should be performed justprior to experiments. Dilution with a carriernucleic acid solution (Yeast RNA) is alsoeffective in improving linearity.Inappropriate thermal conditions. Optimize the thermal conditions according to [5].Reproducibility is notgood.Low purity of the primers or probes.Different lots of primers or probes may showdifferent results. When the lot is changed, priortesting of the primer or probe should beperformed.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************11Symptom Cause SolutionContamination or carry over of the PCR products. Change the contaminated reagent.Amplification from the non-template control(NTC). Inappropriate settings offluorescence measurement, such asin the case of multiplex PCR. In multiplex experiments, inappropriate setting of fluorescence measurement may cause the detection of noise by the cross talk of fluorescent dyes. Settings should be reconfirmed.Excessive amount of ROX reference dye. Excessive amount of ROX reference dye may cause low signal. 50x ROX reference dye should be used according to [5] Table 1.Inappropriate settings of fluorescence measurement. Settings should be confirmed according to the instruction manual of each detector.Low purity of fluorescent probes. Low purity of the probe may increase the baseline. HPLC grade probes should be used.Excessive intensity of the quencher Dye. Certain quenchers (e.g. TAMRA) may cause a higher baseline because of its fluorescence. Use of a non-fluorescent quencher may improve the high baseline.Degradation of the probe. Store the probes according to the manufacture’srecommendations.Insufficient fluorescence measurement time. Certain detection systems require a longer time to detect the fluorescent signal. Longer extension (measurement) time (45-60 sec) may improve the unstable signal.Low amplificationcurve signal /Unstable amplificationcurve signal.Insufficient reaction volume. Low reaction volume may cause an unstablesignal. Increase the reaction volume.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************12[ 8 ] Related productsProduct name Package Code No.High efficiency real-time PCR master mix THUNDERBIRD™ SYBR® qPCR Mix 200reactionsQPS-201High efficiency cDNA synthesis kit for real-time PCR ReverTra Ace® qPCR RT Kit 200reactionsFSQ-101One-step Real-time PCR master mix for probe assayRNA-direct™ Realtime PCR Master Mix 0.5 mL x 20.5 mL x 5QRT-101TQRT-101One-step Real-time PCR master mix for SYBR® Green assayRNA-direct™ SYBR® Realtime PCR Master Mix 0.5 mL x 20.5 mL x 5QRT-201TQRT-201JAPAN CHINA TOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140 www.toyobo.co.jp/e/bio********************NOTICE TO PURCHASER: LIMITED LICENSEA license to perform the patented 5’ Nuclease Process for research is obtained by the purchase of (i) both Authorized 5' Nuclease Core Kit and Licensed Probe, (ii) a Licensed 5’ Nuclease Kit, or (iii) license rights from Applied Biosystems.This product is an Authorized 5’ Nuclease Core Kit. Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152, 5,773,258, 5,407,800, 5,322,770, 5,310,652, 5,210,015, 5,487,972, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. Separate purchase of a Licensed Probe would convey rights under the applicable claims of US Patents Nos. 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, 5,804,375 (claims 1-12 only), and 6,214,979, and corresponding claims outside the United States. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.。

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日本住友化学株式会社product list
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钢铁件环保高效水基除蜡剂的研制

removing agent was determined by orthogonal test as follows: diethanolamine 11 g/L, coconut alkanolamide phosphate
(6503) 9 g/L, fatty alcohol EO-PO polyether 9 g/L, fatty alcohol polyoxyethylene ether (AEO-7) 5 g/L, oleic acid 10 g/L,
通过正交试验确定的除蜡剂钢铁件环保高效水基除蜡剂的研制?373?表表110种表面活性剂的除蜡率cod及及ni残留量table1waxremovalratecodandniresiduefortentypesofsurfactants表面活性剂类型供应商除蜡率codmg?l?1nimg?l?1一乙醇胺阴离子型陶氏化学48111774320二乙醇胺阴离子型陶氏化学669708073三乙醇胺阴离子型陶氏化学541270846866501非离子型巴斯夫652019612356503非离子型巴斯夫6991961366508非离子型巴斯夫68154533973r非离子型巴斯夫733269043aeo3非离子型巴斯夫5618769152aeo7非离子型巴斯夫689761023aeo9非离子型巴斯夫6522585089表表2正交实验结果表table2resultoforthogonaltest实验号二乙醇胺g?l?16503g?l?1rg?l?1aeo7g?l?1除蜡率177557682797782337119979449779735599958866911577737117979248119598129111175891均值1均值2均值3极差79579887681809840819317848168688484884078068最优水平a3b2c3d1992最终配方记为tj33为

2021报关员考试商品归类代码说明

2021报关员考试商品归类代码说明商品名称编码附加编码附加序号说明虫胶 13011000 - 001 -虫胶板 13011000 - 002 -虫胶片 13011000 - 003 -废虫胶 13011000 - 004 -梗胶 13011000 - 005 -片胶 13011000 - 006 -石榴色虫胶 13011000 - 007 -原胶 13011000 - 008 -阿拉伯胶 13012000 - 001 -巴士拉胶 13012000 - 002 -黄蓍胶 13012000 - 003 -尼罗河胶 13012000 - 004 -塞内加尔胶 13012000 - 005 -亚丁胶 13012000 - 006 -腰果树胶 13012000 - 007 -印度胶 13012000 - 008 -胶黄耆树胶 13019010 - 001 -没药 13019020 - 001 -乳香 13019020 - 002 -血竭 13019020 - 003 -阿魏 13019030 - 001 -阿魏胶 13019030 - 002 -松脂 13019040 - 001 -大戟脂 13019090 10 001 -龙血树脂 13019090 10 002 -愈创树脂 13019090 10 003 -氨草胶 13019090 90 001 -安息香胶 13019090 90 002 -巴西胶 13019090 90 003 -贝壳松脂 13019090 90 004 -波斯树脂 13019090 90 005 -草树树脂 13019090 90 006 -达马脂 13019090 90 007 -大麻树脂 13019090 90 008 -刚果胶 13019090 90 009 -黑牵牛子树脂 13019090 90 010 -黄脂石 13019090 90 011 -加拿大香脂 13019090 90 012 -柯巴树脂 13019090 90 013 -柯巴香脂 13019090 90 014 -苦树脂 13019090 90 015 -麦加香脂 13019090 90 016 -秘鲁香脂 13019090 90 017 -山达脂 13019090 90 018 -苏合香脂 13019090 90 019 -塔普香脂 13019090 90 020 -藤黄树脂 13019090 90 021 -妥鲁香脂 13019090 90 022 -香树脂 13019090 90 023 -榄香脂 13019090 90 024 -鸦片浸膏 13021100 - 001 -鸦片液汁 13021100 - 002 -甘草浸膏 13021200 - 001 -甘草液汁 13021200 - 002 -啤酒花浸膏 13021300 - 001 -啤酒花液汁 13021300 - 002 -巴巴斯可根浸膏 13021400 - 001 -除虫菊浸膏 13021400 - 002 -除虫菊液汁 13021400 - 003 -枯杷木浸膏 13021400 - 004 -泡林藤浸膏 13021400 - 005 -鱼藤浸膏 13021400 - 006 -日本漆 13021910 - 001 生的生漆 13021910 - 002 天然漆天然漆 13021910 - 003 -中国漆 13021910 - 004 生的麻黄浸膏粉 13021990 11 001 供制农药用麻黄浸膏 13021990 12 001 供制农药用麻黄浸膏粉 13021990 91 001 供制医药用麻黄浸膏 13021990 92 001 供制医药用麻黄浸膏粉 13021990 93 001 非制农药、医药用麻黄浸膏 13021990 94 001 非制农药、医药用颠茄浸膏 13021990 99 001 -龙胆浸膏 13021990 99 002 -芦荟复合浸膏 13021990 99 003 -天仙子浸膏 13021990 99 004 -天仙子液汁 13021990 99 005 -雄蕨浸膏 13021990 99 006 -雄蕨液汁 13021990 99 007 -药西瓜浸膏 13021990 99 008 -制药用的芦荟液汁 13021990 99 009 -制药用的人参浸膏 13021990 99 010 -制药用的人参液汁 13021990 99 011 -果胶 13022000 - 001 -果胶酸盐 13022000 - 002 -果胶酸脂 13022000 - 003 -琼脂 13023100 - 001 -瓜耳胶天然碱 13023200 - 001 -瓜尔豆胶 13023200 - 002 -农用植物胶 13023200 - 003 -食用植物胶 13023200 - 004 -古俄胶 13023900 - 001 -。

改良Y培养基产品说明书

广东环凯微生物科技有限公司网址：地址：广州市黄埔区科学城神舟路788号邮编：510663传真：860288778876产品说明书Product Manual【产品名称】通用名称：改良Y 培养基英文名称：Modified Yersina Medium 【产品编号与包装规格】产品编号产品类型包装规格029915干粉250g/瓶【产品用途】用于小肠结肠炎耶尔森氏菌的选择性分离和培养。

【检验原理】蛋白胨和水解酪蛋白提供氮源和微量元素；乳糖用于糖发酵试验；氯化钠维持正常的渗透压，去氧胆酸钠和三号胆盐抑制革兰氏阳性菌；丙酮酸钠刺激目标菌的生长；琼脂是培养基的凝固剂。

【使用方法】称取本品67g，加入蒸馏水或去离子水1L，搅拌加热煮沸至完全溶解，分装三角瓶，121℃高压灭菌15分钟。

【质量控制】下列质控菌株接种后于26±1℃培养48h ，观察结果如下表：贮存于避光、干燥处，用后立即旋紧瓶盖；贮存期三年。

【注意事项】1、称量时注意粉尘，佩戴口罩操作以避免引起呼吸道系统不适。

2、干粉培养基使用后立即旋紧瓶盖，避免吸潮结块。

贮存于避光、干燥处。

未开封产品保质期三年。

开封后根据存放条件的不同保质时间存在一定的差异。

3、质检报告可以登录环凯网站，打开“下载中心”页面，输入产品批号下载。

【废物处理】检测之后带菌物品置于121℃下高压灭菌30分钟后处理。

【执行标准】Q/HKSJ03-2011广东环凯微生物科技有限公司企业标准普通微生物培养基【说明版本】2019年11月18日【参考文献】GB4789.8-2016中华人民共和国国家标准食品卫生微生物学检验小肠结肠炎耶尔森氏菌检验广东环凯微生物科技有限公司网址：地址：广州市黄埔区科学城神舟路788号邮编：510663传真：************-8619 ************8602************88778876。

用于工业与公共设施清洗的r几种脂肪醇乙氧基化物的性能研究

用于工业与公共设施清洗的r几种脂肪醇乙氧基化物的性能研究吴霞【摘要】脂肪醇乙氧基化物非离子表面活性剂在清洗剂中常被用做主活性成分,带支链结构的脂肪醇醚产品相对于直链产品具有更强的去污力、更低的泡沫、更好的润湿性能和更低的凝固点.本文主要从基础应用性能方面,探究几种不同结构的非离子表面活性剂在润湿、泡沫、洗涤、硬表面清洗、耐碱和凝固点等方面的性能,并与常规的月桂醇乙氧基化物进行比较.希望对工业和公共设施清洗配方的设计有所帮助.【期刊名称】《中国洗涤用品工业》【年(卷),期】2018(000)005【总页数】8页(P58-65)【关键词】脂肪醇乙氧基化物;应用性能;工业和公用设施清洗【作者】吴霞【作者单位】沙索(中国)化学有限公司,江苏南京,210047【正文语种】中文【中图分类】TQ423.2工业洗涤剂是在工业化生产领域，由于生产技术上的需要而使用的洗涤剂，包括工艺用洗涤剂和非工艺用洗涤剂。

例如在机械加工工业中，对钢材的酸洗（除锈）、碱洗（脱油脂）属于工艺洗涤过程，对机械加工设备的清洗（使用金属清洗剂）属于非工艺洗涤过程。

但是用的洗涤剂都属于工业洗涤剂。

公用设施洗涤剂是适应人类生活社会化，从家用洗涤剂分化出来的一类洗涤剂，用于公用设施及社会化清洁服务。

其洗涤基质大都为有规则的硬表面，洗涤过程由专职人员来承担。

例如，承担社会清洁服务的洗衣房使用有别于家用织物洗涤剂的一类洗涤用品，承担建筑物清洗，宾馆、剧场、车站等公共场所的专业清洗公司，一般使用机械化方式和专用洗涤剂完成清洗工程。

服务行业中与洗涤有密切关系的如餐饮业洗涤餐具、厨具、灶具；医院中洗涤手术器械、病床用织物，手术前洗手消毒；公共场所洗涤厕所、卫生间，都需要较家用洗涤剂洗涤消毒性能更好的专用产品。

工业与公共设施清洗剂中使用的原料范围很宽，种类很多，这是因为工业及公共设施洗涤工艺中，面对的基质和污垢要复杂得多，洗涤现场千差万别，对洗涤剂的技术要求也参差不齐，但是使用频率最高的仍然是表面活性剂。