AF38469_1531634-31-7_DataSheet_MedChemExpress
游离雌三醇测定试剂盒(时间分辨荧光免疫分析法)产品技术要求模板
医疗器械产品技术要求编号：游离雌三醇测定试剂盒（时间分辨荧光免疫分析法）1.产品型号/规格及划分说明1.1产品型号/规格96人份/盒（半自动）、96人份/盒（全自动）。
1.2结构组成试剂盒由uE3校准品（冻干粉）、uE3标记物（冻干粉）、uE3抗体、uE3实验缓冲液、浓缩洗液、增强液和uE3微孔反应板组成。
1.3 适用范围用于定量检测人体血清中的游离雌三醇含量。
2.性能指标2.1装量实验缓冲液、增强液、浓缩洗液的最大允许负偏差为 6.0％。
2.2外观试剂盒中校准品、标记物为冻干粉，呈白色或者淡黄色粉末或块状物，复溶后为澄清或浅黄色液体，不应含异物、混浊或摇不散的沉淀；其它液体组份试剂均为澄清透明；微孔反应板应封口良好，无破损。
2.3最低检测限试剂盒的最低检测限应不高于 0.6 nmol/L。
2.4特异性）交叉反应系数应不高于 2%；与孕酮（P）交叉反应系数应不高于与雌二醇（E22%。
2.5线性相关系数试剂（盒）的线性范围（0.6～49.2）nmol/L 内的线性应符合如下要求：a)线性相关系数 r≥0.990；b)检测浓度＜4 nmol/L 的uE3 时，线性的绝对偏差的绝对值应在 0.5 nmol/L 的范围内；检测浓度≥4 nmol/L 的uE3 时，线性相对偏差的绝对值≤15%。
2.6校准品的准确性用国家标准品制备的二级线性校准品（注册检验时使用）或经国家标准品标化的企业参考品对产品校准品（零值除外）的浓度进行测定，产品校准品 B、C 点的实测浓度与标示浓度绝对偏差的绝对值应在 0.5 nmol/L 的范围内，产品校准品 D～F 点的实测浓度与标示浓度相对偏差的绝对值≤20%。
2.7测量准确度用试剂盒对检测范围内低、中两个浓度人血清样本进行回收实验，其回收率应在（85%~115%）范围内。
2.8测量精密度2.8.1 校准品不精密度（均一性）试剂盒校准品（零值除外）B 点、C 点的不精密度（CV）应不超过 20.0%；D 点、E 点、F 点的不精密度（CV）应不超过 15.0%。
IVD行业国外原料主要供应商
.aaltobioreagents.ie .aaltoscientific..aetltd..biocell..npods.ru.diarect..endocrinetech..scipac..eastcoastbio..haemtech. .immunovision..mainebiotechnology. .operon.es.equitech-bio..quadfive..promeddx..seracare..chemogen..modiquest..seramon..midlandbio..capricornproducts. .instruchemie.nl.sheffield-products. .biogenes.de.biocheckinc..biospacific..bioprocessinginc..fitzgerald-fii..microbix..inventdiagnostica.de .biomarket.fi.calbioreagents..xema-medica..scrippslabs..silverlakeresearch..ssi.dk.virostat-inc..virusys..oycus..accessbiologicals. .anshlabs..arlingtonscientific..auditmicro..brt-us..cardinalbiologicals. .diasource.be.diazyme..dsitaly..icllab..immunoreagents. .magsphere.丹麦提供诊断试剂盒和抗体、抗原和血清，有特色的产品是 CE认证的NGAL诊断试剂盒，MBL试剂盒重症监护和止血，临床化学仪器，试剂盒日本提供诊断试剂盒产品的公司，特色产品是低密度LDL 和胱抑素C 试剂盒。
产品涉及质控品，转染病，糖尿病，肿瘤，生殖，甲状腺等试剂盒Acris 是一家德国的著名抗体公司，提供近 3 万种各种优质抗体、蛋白及抗体纯化试剂盒，产品X 围涉及免疫学、细胞生物学、细胞神经信号传导、蛋 白组学、肿瘤生物学等。
碧云天细胞自噬染色检测试剂盒(MDC法)说明书
细胞自噬染色检测试剂盒(MDC 法)产品简介：碧云天生产的细胞自噬染色检测试剂盒(MDC 法)，即Autophagy Staining Assay Kit with MDC ，是一种使用丹酰尸胺，也称单丹磺酰尸胺、丹酰尸胺或丹酰戊二胺(monodansylcadaverine, MDC)作为荧光探针快速便捷地检测细胞自噬的试剂盒。
自噬(autophagy)是一种在进化上高度保守的通过溶酶体吞噬并降解部分自身组分的细胞内分解代谢途径。
自噬与多种生理功能有关，在饥饿等环境条件下，细胞通过自噬降解多余或异常的细胞内组分，为细胞的生存提供能量及原材料，促进生物体的生长发育、细胞分化及对环境变化产生应答。
自噬异常与多种病理过程如肿瘤、神经退行性疾病、代谢疾病、病原体感染等都有密切关系。
由于细胞自噬在生理和病理过程中都有重要作用，自噬已经成为细胞生物学领域的一个研究热点。
MDC 是细胞自噬检测最常用的荧光探针之一。
MDC 可以通过离子捕获(ion trapping)和与膜脂的特异性结合，从而特异性标记自噬体(autophagosome)，也称autophagic vacuole ，因而常用于细胞自噬的检测。
MDC 是一种嗜酸性荧光探针，很多酸性膜性结构也会被MDC 染色，因此MDC 染色时正常的细胞也会有一定的染色背景。
本产品的染色原理决定了本产品只能用于培养的细胞或者组织的细胞自噬荧光染色检测，不能用于冻存的或固定的细胞、组织或者组织切片的染色检测。
使用本产品染色后可以通过荧光显微镜拍照观察，也可以通过荧光酶标仪或流式细胞仪进行荧光检测。
荧光显微镜观察时可以使用紫外区激发光激发，发出绿色荧光。
荧光酶标仪或流式细胞仪推荐的激发波长为335nm (330-360nm 均可)，发射波长为512nm (510-540nm 均可)。
本产品用于细胞自噬染色的效果参考图1。
图1. 细胞自噬染色检测试剂盒(MDC 法)的染色效果图。
碧云天生物技术 Beyotime Biotechnology 生物素标记EMSA探针说明书
碧云天生物技术/Beyotime Biotechnology订货热线：400-168-3301或800-8283301订货e-mail：******************技术咨询：*****************网址：碧云天网站微信公众号生物素标记EMSA探针－β-Catenin/TCF (0.2μM)产品编号产品名称包装GS018B 生物素标记EMSA探针－β-Catenin/TCF (0.2µM) 200µl产品简介：生物素标记EMSA探针－β-Catenin/TCF是用于EMSA(也称gel shift)研究的生物素(Biotin)标记的β-Catenin/TCF consensus oligonucleotide。
这个生物素标记的双链寡核苷酸含有公认的β-Catenin/TCF结合位点，可以用作EMSA研究时的探针。
β-Catenin/TCF consensus oligo的序列如下：5'-CCC TTT GAT CTT ACC-3'3'-GGG AAA CTA GAA TGG-5'本生物素标记EMSA探针已经过纯化，可以直接用于EMSA结合反应。
本生物素标记EMSA探针可以和碧云天的化学发光法EMSA试剂盒(GS009)配套使用。
一个包装的生物素标记探针可以进行约200-400个样品的EMSA检测。
包装清单：产品编号产品名称包装GS018B 生物素标记EMSA探针－β-Catenin/TCF (0.2µM) 200µl—说明书1份保存条件：-20ºC保存，一年有效。
注意事项：避免加热到40ºC以上，温度过高会导致双链DNA探针解聚成单链。
而单链无法用于EMSA研究。
对于基于生物素标记的EMSA检测的详细操作可以参考碧云天的化学发光法EMSA试剂盒(GS009)的使用说明。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
Amtech Tacky 助焊膏系列安全数据表说明书
Inventec Performance Chemicals USA, LLCSAFETY DATA SHEET (SDS)SECTION 1: PRODUCT AND COMPANY IDENTIFICATIONPRODUCT NAME: Amtech Tacky Paste Flux Series: 200, 400, 500, 600, 4000, SynTECH, WSFC-305L and #61 SYNONYMS:Tacky FluxMANUFACTURER: Inventec Performance Chemicals USA, LLCADDRESS:PO Box 989 Deep River, CT 06417 USAPHONE:860-526-8300FAX:860-526-8243EMERGENCY:Infotrac-(800)535-5035REVISION DATE:December 19, 2014REVISION DATE: 3DOCUMENT NAME:SDS-Tacky Flux-008PRODUCT USE:Bonding solder joints in production and repair of circuit boardsSECTION 2: HAZARDS IDENTIFICATIONCHEMICAL NAME:N/ACHEMICAL FAMILY:MixtureCHEMICAL FORMULA:N/AROUTES OF ENTRY: Inhalation, Ingestion, Skin/Eye ContactGHS:Signal Word: WarningHazard statement(s)H302 Harmful if swallowedH317 May cause an allergic skin reactionH320 Causes eye irritationH335 May cause respiratory irritationPrecautionary statement(s)P102 Keep out of reach of childrenP233 Keep container tightly closedP264 Wash hands thoroughly after handlingP270 Do not eat, drink or smoke when using this productP280 Wear protective gloves/protective clothing/eye protection/face protectionP302+P352 IF ON SKIN: Wash with plenty of soap and waterP305+P351 IF IN EYES: Rinse continuously with water for several minutesP404 Store in a closed containerP501 Dispose of contents/containers in accordance with Federal, State/Provincial, and/or local regulations POTENTIAL HEALTH EFFECTS:EYE CONTACT: May cause moderate irritation. Do not allow material to come in contact with eyes.SKIN CONTACT: May cause moderate skin irritation.INHALATION: May cause irritation to the respiratory tract.INGESTION: Harmful if swallowed. May cause irritation to the mouth, throat, and stomach. May cause abdominal discomfort, nausea, vomiting, and/or diarrhea.CHRONIC: Not established.SECTION 2 NOTES:Inventec Performance Chemicals USA, LLC does not recommend, manufacture, market, or endorse any of its products for human consumption.SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTSIngredient CAS Number Exposure LimitsModified Rosins N/A N/APine Oil Derivatives 8000-41-7 N/AProprietary Ingredients N/A N/AMixed Carboxylic Acids N/A N/ASECTION 3 NOTES:Percentages of individual components are not listed as this information is considered a trade secret.SECTION 4: FIRST AID MEASURESEYES: Flush with plenty of water, contact a physician. If contact lenses can be removed easily, flush eyes without contact lenses. SKIN: Wash affected area with plenty of warm, soapy water. If irritation persists, seek medical attention.INGESTION: Call a physician or Poison Control Center immediately. Do not induce vomiting.INHALATION: Remove to fresh air. If not breathing, seek immediate medical attention.SECTION 5: FIRE-FIGHTING MEASURESEXTINGUISHING MEDIA: Dry chemical, foamSPECIAL FIRE FIGHTING PROCEDURES: Do not use water. Use NIOSH-approved self-contained Breathing Apparatusand full protective clothing if involved in a fire.UNUSUAL FIRE AND EXPLOSION HAZARDS:This product does not present any unusual fire and explosion hazards. SECTION 6: ACCIDENTAL RELEASE MEASURESACCIDENTAL RELEASE MEASURES: If material spills or leaks, collect and place into a properly labeled waste container. Remove traces of tacky flux using cloth rags or paper towels moistened with Isopropyl Alcohol. Follow on-site personal protective equipment recommendations.SECTION 6 NOTES:See Sections 2, 4, and 7 for additional information.SECTION 7: HANDLING AND STORAGEHANDLING/STORAGE: Keep containers tightly closed when not in use. Use care to avoid spills. Avoid inhalation of fumes or dust. Avoid contact with eyes, skin, and clothing.OTHER PRECAUTIONS: Empty containers may retain product residues in vapor, liquid, and/or solid form. All labeled hazard precautions should be observed.WORK HYGIENIC PRACTICES: Cosmetics/Food/Drink/Tobacco should not be consumed or used in work areas. Always wash hands after handling material and before applying or using cosmetics/food/drink/tobacco.SECTION 7 NOTES:For industrial use only.SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTIONVENTILATION: Provide sufficient mechanical (general and/or local exhaust) ventilation to maintain exposure below TLVs. RESPIRATORY PROTECTION: Use with adequate ventilation.EYE PROTECTION: Use with appropriate safety glasses.SKIN PROTECTION: Protective gloves and clothing should be worn when handling material. Wash hands thoroughly with soap and water upon leaving the work area.SECTION 9: PHYSICAL AND CHEMICAL PROPERTIESAPPEARANCE: Clear, White, or Yellow to Dark Amber gelODOR: Mild odorODOR THRESHOLD: Not establishedpH as SUPPLIED: N/ASECTION 9: PHYSICAL AND CHEMICAL PROPERTIES (continued)MELTING POINT: Not establishedFREEZING POINT: Not establishedINITIAL BOILING POINT: Not establishedBOILING RANGE: Not establishedFLASH POINT: Not establishedEVAPORATION RATE: Not establishedFLAMMABILITY (solid): Not establishedUPPER/LOWER FLAMMABILITY: Not establishedUPPER/LOWER EXPLOSIVE LIMITS:Not establishedVAPOR PRESSURE (mmHg): N/A (°F/°C)VAPOR DENSITY (AIR = 1): N/A (°F/°C)RELATIVE DENSITY: Not establishedSOLUBILITY IN WATER: PartiallyPARTITION COEFFICIENT (n-octanol/water): Not establishedAUTOIGNITION TEMPERATURE: Not establishedDECOMPOSITION TEMPERATURE: Not establishedVISCOSITY: N/A (°F/°C)SECTION 10: STABILITY AND REACTIVITYSTABILITY: StableCONDITIONS TO AVOID (STABILITY): Freezing temperatures. High temperatures. INCOMPATIBILITY (MATERIAL TO AVOID): Strong oxidizing materialsHAZARDOUS DECOMPOSITION/BY-PRODUCTS: Harmful organic fumes and toxic oxide fumes may form at elevatedtemperatures.POSSIBILITY OF HAZARDOUS REACTIONS: Will not occurSECTION 11: TOXICOLOGICAL INFORMATIONACUTE TOXICITY: Not availableSKIN CORRISION/IRRITATION: Not establishedSERIOUS EYE DAMAGE/IRRITATION: Not availableRESPIRATORY OR SKIN SENSITIZATION: Not establishedGERM CELL MUTAGENICITY: Not availableCARCINOGENICITY: Not availableREPRODUCTIVE TOXICITY: Not availableSTOT-SINGLE EXPOSURE: Not availableSTOT-REPEATED EXPOSURE: Not availableASPIRATION HAZARD: Not availableSECTION 12: ECOLOGICAL INFORMATIONTOXICITY: Product not testedPERSISTENCE AND DEGRADIBILITY: Product not testedBIOACCUMULATIVE POTENTIAL: Product not testedMOBILITY IN SOIL: Product not testedOTHER ADVERSE EFFECTS: Product not testedSECTION 13: DISPOSAL CONSIDERATIONSWASTE DISPOSAL METHOD: Scrap and waste solder should be stored in a dry, sealed container for later disposal. Disposal must be in accordance with Federal, State/Provincial, and Local Regulations.SECTION 14: TRANSPORT INFORMATIONTransport in accordance with applicable regulations and requirements.UN Number: Not availableUN Proper Shipping Name: Not availablePackaging Group:Not applicableEnvironmental Hazards:NoneTRANSPORT HAZARD CLASSES:US DOT Hazardous Material Classification: Tacky Flux is not listed as a DOT hazardous materialWater Transportation: Tacky Flux is not listed as a hazardous materialIATA Hazardous Material Classification: Tacky Flux is not listed as IATA hazardous materialSECTION 15: REGULATORY INFORMATIONAll ingredients used to manufacture this product are listed on the EPA TSCA Inventory.U.S. FEDERAL REGULATIONS: Not regulatedSTATE REGULATIONS: Not regulatedINTERNATIONAL REGULATIONS: Not regulatedSECTION 16: OTHER INFORMATIONHMIS Rating: Health=1 Flammability=1 Physical Hazard=0 Personal Protection=X KEY:N/A: Not applicableGHS: Global Harmonized SystemOSHA: Occupational Safety and Health AdministrationACGIH: American Conference of Governmental Industrial HygienistsNTP: National Toxicology ProgramIARC: International Agency for Research on CancerCAS: Chemical Abstract ServiceNIOSH: National Institute for Occupational Safety & HealthSTOT: Specific target organ toxicityTLV: Threshold limit valueUS DOT: United States Department of TransportationDOT: Department of TransportationIATA: International Air Transport AssociationEPA:Environmental Protection AgencyTSCA:Toxic Substance Control ActHMIS:Hazardous Material Identification SystemPREPARATION INFORMATION:This update supersedes all previously released documents.PREPARED BY: Wendy W. GesickAPPROVED BY: Leigh W. GesickDISCLAIMER:The information contained herein is based on data considered to be accurate but does not purport to be all-inclusive and shall be used only as a guide. No warranty is expressed or implied regarding the accuracy of this data and Inventec Performance Chemicals USA, LLC shall not be held liable for any damage resulting from any handling or contact with the above product. Liability is expressly disclaimed for loss or injury arising out of use of this information or the use of any materials designated. This material is not for resale, unauthorized distribution, or personal use.。
美国贝克曼库尔特流式细胞分析仪
美国贝克曼库尔特流式细胞分析仪（Beckman coulter cell）产品型号：Cell Lab Quanta SC当前价格：0.00元产品数量：0新旧程度：全新有效期至：0000-00-00所在地：产品简介：仪器简介：T细胞亚群检测的CD45/CD4/CD8/CD3、CD45/CD56/CD19/CD3；阵发性血红蛋白尿（PNH）检测的CD55、CD59；血小板无力症（GT）检测的CD41、CD61等等详细信息仪器简介：T细胞亚群检测的CD45/CD4/CD8/CD3、CD45/CD56/CD19/CD3；阵发性血红蛋白尿（PNH）检测的CD55、CD59；血小板无力症（GT）检测的CD41、CD61等等。
但对于白血病/淋巴瘤免疫分型，国际上迄今为止也没有统一的抗体组合。
在2000年国际细胞分析学会（ISAC）大会上，临床血细胞计数协会组织了一次国际专家会议，以期对检测血液淋巴系统肿瘤所需最少、最有效的单抗数达成共识。
75%与会者一致认为，对于慢性淋巴系统增殖性疾病（CLD）有9种单抗：CD5,CD19,κ,λ,CD3,CD20,CD23,CD10,CD45对初诊来说是最基本的。
淋巴瘤和CLD相似，需要至少12-16种单抗。
对于急性白血病（AL），75%的与会者认为大约13-15种单抗是最基本的：CD10,CD19,CD79a,CD13,CD33,CD34,CD45,CD2,MPO，CD7,CD14,CD3,HLA-DR等，对初步鉴别白血病系列是必需的。
其他一些（CD16,CD56,CDw65,TdT,cyCD3）可能对某些病例有用。
几乎所有的投票者都认为，要对急性白血病完善分类所需单抗的恰当数量平均为20-24种。
但这些抗体之间组合也是一大难题，目前也无统一规定（如表二）。
大会多数发言者(11/13)指出，对已确诊病人的监护和分期来说，仅需较少单抗。
抗体的质量控制是实验的关键环节。
抗体的质量包括其特异性、灵敏度、精密度。
QPCR及QRT-PCR系列产品
Invitrogen的ICFC系列产品促销1．QPCR及QRT-PCR系列产品Invitrogen公司专门为中国客户提供的定量PCR试剂盒，结合了 UDG 防止残余污染技术和SYBR® Green I 荧光染料（存在于SYBR® Green I荧光定量PCR试剂盒中），在美国接受了严格的质量监控，可提供极高灵敏度的目的序列定量检测，线性剂量低，反应浓度范围很大。
qPCR Supermix-- 即用型反应剂，专为高特异性、实时定量DNA扩增设计UDG-- 防止携带污染物，减少克隆片段假阳性结果ROX参考染料-- 适用ABI仪器的校正染料产品信息活动时间：即日起至2009年4月30日2．Gibco南美胎牛血清即日起凡优惠价¥1780购买Gibco胎牛血清500ml（目录号:C2027050）即可获赠送价值¥250现金抵用券。
您可以凭现金抵用券在英韦创津公司购买任何商品，此券有效期至2009年5月31日。
产品信息活动时间：即日起至2009年4月30日独特的采集方式：GIBCO采用无菌心脏穿刺的方式采血原装直送，避免污染：原产地采集、加工、检测、包装。
完善的质控：采集、处理、检测、运输等环节都有文件和证书。
3．Invitrogen TA Cloning克隆产品专门用于克隆Taq聚合酶扩增的PCR产物。
采用pCR载体，能产生80%以上的重组产物，90%以上重组产物都包含插入片段。
产品信息活动时间：即日起至2009年5月31日附：pCR载体优点及图谱：3’-T突出端可直接连接Taq扩增的PCR产物可选择T7或T7和Sp6启动子进行体外RNA转录和测序侧向EcoRⅠ位点的通用多接头位点方便了插入片段的切离可以选择卡那霉素或氨苄青霉素进行筛选非常简便的蓝/白克隆筛选具有M13正向和反向引物位点，方便测序4．GIBCO液体培养基系列产品创立近50年的历史，品质优秀，产品种类丰富；为了中国用户利益，特建立国内生产线；所有产品，从原材料到生产全部按照GIBCO质量标准进行，每批均送抵美国公司总部质检合格后，才在国内销售。
叶酸ELISA试剂盒产品手册说明书
Product ManualFolic Acid ELISA KitCatalog NumberMET-5068 96 assaysMET-5068-5 5 x 96 assays FOR RESEARCH USE ONLYNot for use in diagnostic proceduresIntroductionFolic acid is a B vitamin also known as Vitamin B9. Since humans don’t synthesize folic acid, it is required from the diet and is therefore considered to be an essential vitamin. In cells, folic acid is required for amino acid metabolism as well as to carry one-carbon groups used for methylation reactions and synthesis of nucleic acids (such as thymine and purine bases). Therefore, deficiency in folic acid disrupts DNA synthesis and cell division, affecting mostly hematopoietic cells and abnormal tissue growths because of their higher rate of cell division.Folic acid is used to supplement folic acid deficiency. This deficiency can cause anemia. Symptoms of anemia can include fatigue, heart palpitations, difficulty breathing, open sores observed on the tongue, and color changes of the hair or skin. Deficiency can occur in children after only a month of consuming a folic acid deficient diet. In adults, normal total body folic acid levels are between10,000–30,000 micrograms (µg) with plasma levels of greater than 7 nM (3 ng/mL) (Table 1). Women also take supplemental folic acid during pregnancy to prevent fetal neural tube defects (NTDs). Insufficient levels of dietary folic acid in early pregnancy are thought to be the cause of over half of babies born with neural tube defects. As a result, over 50 countries add folic acid to certain foods as a way to decrease NTD incidents in the population.Concentration (ng/mL) Concentration (nM) Adults 3-20 7-45.3Children 5-21 11.3-47.6Infants 14-51 31.7-115.5Table 1. Reference ranges for folic acid in human plasma.The Folic Acid ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of folic acid in serum, cell or tissue samples. The quantity of folic acid in unknown samples is determined by comparing its absorbance with that of a known folic acid standard curve. The kit has detection sensitivity limit of 24 pg/mL folic acid. Each Folic Acid ELISA Kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples. Assay PrincipleThe Folic Acid ELISA kit is a competitive ELISA for the quantitative measurement of folic acid. The unknown folic acid samples or folic acid standards are first added to a Folic Acid Conjugate preabsorbed microplate. After a brief incubation, an Anti-Folic Acid antibody is added, followed by an HRP conjugated secondary antibody. The folic acid content in unknown samples is determined by comparison with a predetermined folic acid standard curve.Related Products1.MET-5054: L-Amino Acid Assay Kit2.MET-5056: Branched Chain Amino Acid Assay Kit3.MET-5151: S-Adenosylhomocysteine (SAH) ELISA Kit4.MET-5152: S-Adenosylmethionine (SAM) ELISA Kit5.STA-674: Glutamate Assay KitKit ComponentsBox 1 (shipped at room temperature)1.96-well Protein Binding Plate (Part No. 231001): One strip well 96-well plate.2.Anti-Folic Acid Antibody (500X) (Part No. 50681C): One 10 µL vial.3.Secondary Antibody, HRP Conjugate (Part No. 231009): One 20 µL vial.4.Assay Diluent (Part No. 310804):One 50 mL bottle.5.10X Wash Buffer (Part No. 310806): One 100 mL bottle.6.Substrate Solution (Part No. 310807): One 12 mL amber bottle.7.Stop Solution (Part. No. 310808): One 12 mL bottle.8.Folic Acid Standard (Part No. 50682C): One 100 µL amber vial of 10 µg/mL Folic Acid inwater.Box 2 (shipped on blue ice packs)1.100X Folic Acid Conjugate (Part No. 50683C): One 100 µL amber vial.Materials Not Supplied1.Folic acid samples such as serum, plasma, or folic acid extracted from cells or tissues2.Tissue Homogenizer3.1X PBS4.10 µL to 1000 µL adjustable single channel micropipettes with disposable tips5.50 µL to 300 µL adjustable multichannel micropipette with disposable tips6.Multichannel micropipette reservoir7.Microplate reader capable of reading at 450 nm (620 nm as optional reference wave length) StorageUpon receipt, aliquot and store 100X Folic Acid Conjugate at -20ºC and avoid multiple freeze/thaw cycles. Store all other components at 4ºC. The 100X Folic Acid Conjugate and Folic Acid Standard are light sensitive and must be stored accordingly.Preparation of Reagents•Folic Acid Conjugate Coated Plate: Dilute the proper amount of 100X Folic Acid Conjugate 1:100 into 1X PBS. Add 100 μL of the diluted 1X Folic Acid Conjugate to each well and incubate at 37ºC for two hours or overnight at 4ºC. Remove the coating solution and wash twice with 200 μL of 1X PBS. Blot plate on paper towels to remove excess fluid. Add 200 μL of Assay Diluent to each well and block for 1 hr at room temperature. Transfer the plate to 4ºC and remove the Assay Diluent immediately before use.Note: The Folic Acid Conjugate-coated wells are not stable and should be used within 24 hrs after coating. Only coat the number of wells to be used immediately.•1X Wash Buffer: Dilute the 10X Wash Buffer to 1X with deionized water. Stir to homogeneity. •Anti-Folic Acid Antibody and Secondary Antibody: Immediately before use dilute the Anti-Folic Acid Antibody 1:500 and Secondary Antibody 1:1000 with Assay Diluent. Do not store diluted solutions.Preparation of Standard Curvee the provided stock Folic Acid Standard 10 µg/mL solution to prepare a series of the remainingstandards according to Table 1 below.Standard Tubes 10 µg/mL Folic AcidStandard (µL)Assay Diluent(µL)Folic Acid(ng/mL)Folic Acid(nM)1 10 990 100 2272 100 of Tube #1 300 25 56.753 100 of Tube #2 300 6.25 14.194 100 of Tube #3 300 1.56 3.555 100 of Tube #4 300 0.391 0.8876 100 of Tube #5 300 0.098 0.2227 100 of Tube #6 300 0.024 0.0558 0 300 0 0 Table 1. Preparation of Folic Acid Standards.Preparation of Samples•Serum: Avoid hemolyzed and lipemic blood samples. Collect blood in a tube with no anticoagulant. Allow the blood to clot at room temperature for 30 minutes. Centrifuge at 2500 x g for 20 minutes. Remove the yellow serum supernatant without disturbing the white buffy layer.Aliquot samples for testing and store at -80ºC. Perform dilutions in Assay Diluent or PBS containing 0.1% BSA as necessary.•Plasma: Avoid hemolyzed and lipemic blood samples. Collect blood with heparin or citrate and centrifuge at 2000 x g and 4ºC for 10 minutes. Remove the plasma layer and store on ice. Avoid disturbing the white buffy layer. Aliquot samples for testing and store at -80ºC. Perform dilutions in Assay Diluent or PBS containing 0.1% BSA as necessary.Note: This assay is not compatible with rabbit serum or plasma due to high levels of rabbit IgG that will cross react with the secondary antibody.•Cells or tissues: Homogenize 50-200 mg of the cell pellet or tissue in 0.5-2 mL of ice-cold PBS using a mortar and pestle or by dounce homogenization. Incubate the homogenate at 4°C for 20 minutes. Transfer the homogenate to a centrifuge tube and centrifuge at 12000 x g for 20 minutes.Recover the supernatant and transfer to a fresh tube. Store resuspended sample at -20°C or colder until ready to test by ELISA. Perform dilutions in Assay Diluent or PBS containing 0.1% BSA as necessary.Assay Protocol1.Prepare and mix all reagents thoroughly before use. Each folic acid sample including unknownand standard should be assayed in duplicate.2.Add 50 µL of unknown sample or Folic Acid standards to the wells of the Folic Acid Conjugatecoated plate. Incubate at room temperature for 10 minutes on an orbital shaker.3.Add 50 µL of the diluted Anti-Folic Acid antibody to each well, incubate at room temperature for 1hour on an orbital shaker.4.Wash microwell strips 3 times with 250 µL 1X Wash Buffer per well with thorough aspirationbetween each wash. After the last wash, empty wells and tap microwell strips on absorbent pad or paper towel to remove excess 1X Wash Buffer.5.Add 100 µL of the diluted Secondary Antibody-HRP Enzyme Conjugate to all wells.6.Incubate at room temperature for 1 hour on an orbital shaker.7.Wash microwell strips 3 times according to step 4 above. Proceed immediately to the next step.8.Warm Substrate Solution to room temperature. Add 100 L of Substrate Solution to each well,including the blank wells. Incubate at room temperature on an orbital shaker. Actual incubation time may vary from 2-30 minutes.Note: Watch plate carefully; if color changes rapidly, the reaction may need to be stopped sooner to prevent saturation.9.Stop the enzyme reaction by adding 100 µL of Stop Solution into each well, including the blankwells. Results should be read immediately (color will fade over time).10.Read absorbance of each microwell on a spectrophotometer using 450 nm as the primary wavelength.Example of ResultsThe following figures demonstrate typical Folic Acid ELISA results. One should use the data below for reference only. This data should not be used to interpret actual results.Figure 1: Folic Acid ELISA Standard Curve.Figure 2: Folic Acid Levels in Human, Mouse or Rat Serum compared to Negative Control (Assay Diluent). Serum samples were diluted 1:5 in Assay Diluent and tested according to the Assay Protocol.References1.Bibbins-Domingo, K; Grossman, DC.; Curry, SJ.; Davidson, KW.; Epling, John W.; García, FAR.;Kemper, AR.; Krist, AH.; Kurth, AE.; Landefeld, CS; Mangione, CM.; Phillips, William R.;Phipps, MG.; Pignone, MP.; Silverstein, M; Tseng, C-W (2017). JAMA. 317: 183.2.Marino, BS.; Fine, KS (2009). Blueprints Pediatrics. Lippincott Williams & Wilkins. p. 131.3.Bailey, LB. (2009). Folate in Health and Disease, Second Edition. CRC Press. p. 198.4.Obeid, R; Herrmann, W (2012). Curr. Drug Metab. 13: 1184–1195.5.Wilson RD, Wilson RD, Audibert F, Brock JA, Carroll J, Cartier L, Gagnon A, Johnson JA,Langlois S, Murphy-Kaulbeck L, Okun N, Pastuck M, Deb-Rinker P, Dodds L, Leon JA, Lowel HL, Luo W, MacFarlane A, McMillan R, Moore A, Mundle W, O'Connor D, Ray J, Van den Hof M (2015). J Obstet Gynaecol Can. 37: 534–52.6.Figueiredo JC1, Grau MV, Haile RW, Sandler RS, Summers RW, Bresalier RS, Burke CA,McKeown-Eyssen GE, Baron JA. J. Natl. Cancer Inst.101: 432–5.Recent Product Citations1.McCarthy, G.A. et al. (2023). A Novel 3DNA® Nanocarrier effectively delivers payloads topancreatic tumors. Transl Oncol. 32:101662. doi: 10.1016/j.tranon.2023.101662.2.Shinagawa, A. et al. (2022). Short-Term Combined Intake of Vitamin B2 and Vitamin E DecreasesPlasma Homocysteine Concentrations in Female Track Athletes. Dietetics. 1(3):216-226. doi:10.3390/dietetics1030019.3.Siervo, M. et al. (2020). Nitrate-Rich Beetroot Juice Reduces Blood Pressure in Tanzanian Adultswith Elevated Blood Pressure: A Double-Blind Randomized Controlled Feasibility Trial. J Nutr.doi: 10.1093/jn/nxaa170.4.Simanjuntak, Y. et al. (2020). Preventive effects of folic acid on Zika virus-associated poorpregnancy outcomes in immunocompromised mice. PLoS Pathog. 16(5):e1008521. doi:10.1371/journal.ppat.1008521.5.Zhu, J. et al. (2018). Effect of maternal folic acid supplementation on prostatitis risk in the ratoffspring. Int Urol Nephrol. 50(11):1963-1973. doi: 10.1007/s11255-018-1969-8.WarrantyThese products are warranted to perform as described in their labeling and in Cell Biolabs literature when used in accordance with their instructions. THERE ARE NO WARRANTIES THAT EXTEND BEYOND THIS EXPRESSED WARRANTY AND CELL BIOLABS DISCLAIMS ANY IMPLIED WARRANTY OF MERCHANTABILITY OR WARRANTY OF FITNESS FOR PARTICULAR PURPOSE. CELL BIOLABS’sole obligation and purchaser’s exclusive remedy for breach of this warranty shall be, at the option of CELL BIOLABS, to repair or replace the products.In no event shall CELL BIOLABS be liable for any proximate, incidental or consequential damages in connection with the products.Contact InformationCell Biolabs, Inc.7758 Arjons DriveSan Diego, CA 92126Worldwide: +1 858-271-6500USA Toll-Free: 1-888-CBL-0505E-mail: ********************©2017-2023: Cell Biolabs, Inc. - All rights reserved. No part of these works may be reproduced in any form without permissions in writing.。
beyotime的alp的catalog numbers
beyotime的alp的catalog numbersBeyotime是一家专业从事蛋白质组学、生物化学、细胞生物学等领域的生物试剂制造商，其ALP试剂盒是研究蛋白质和酶的理想选择。
本文将围绕Beyotime的ALP目录号展开，从多个角度为您介绍。
一、目录号的概念及其作用目录号（Catalog number）是Beyotime对生物试剂进行标识的唯一编号。
每种试剂都有一个独特的目录号，它由一定长度的字母和数字组成。
通过这个编号，电子商务平台或供应商可以非常方便地确定所需的试剂种类和批次，确保拿到的试剂是无误的。
二、Beyotime ALP试剂盒的种类1.磷酸酶酶标测定试剂盒：用于在细胞发育、代谢和疾病过程中检测磷酸酶活性。
目录号为P0321。
2.骨髓碱性磷酸酶（BALP）活性分析试剂盒：用于测定血清、血浆和尿液中的BALP活性。
目录号为P7607。
3.骨钙素（BGLAP）酶联免疫分析试剂盒：用于测定人类和小鼠血清、血浆和培养上清中的BGLAP水平。
目录号为EK0413。
4.乳铁蛋白（LTF）酶联免疫分析试剂盒：用于测定人类和小鼠血清、血浆和培养上清中的LTF水平。
目录号为EK0981。
5.核糖核酸酶P26（RNASP26）酶联免疫分析试剂盒：用于测定人类和小鼠血清、血浆和培养上清中的RNASP26水平。
目录号为EK3721。
三、如何选择适合自己的ALP试剂盒1.确定所需检测的样本类型ALP试剂盒针对不同的样本类型进行设计，如血清、血浆、尿液、培养上清等，因此在购买时需要明确所需样本。
2.选择对应种类的ALP试剂盒根据需要检测的目标蛋白，从Beyotime提供的ALP试剂盒中选择合适的一种。
3.确定所需的检测量级Beyotime的ALP试剂盒一般都有不同的检测范围，根据需求选择合适的检测级别。
4.确认批号和有效期选购时需要仔细查看目录号的前两位，确保购买的是同一批次的试剂，同时需要确认有效期，过期试剂会影响检测结果。
糖尿病患者诊断应用血清C肽及糖化血红蛋白联合检测的价值分析
DOI：10.16658/ki.1672-4062.2023.14.085糖尿病患者诊断应用血清C肽及糖化血红蛋白联合检测的价值分析倪胜南，陈少，陈一鸣泗阳康达医院检验科，江苏宿迁223700[摘要]目的探讨糖尿病患者诊断应用血清C肽联合糖化血红蛋白检测的价值。
方法将2022年1月—2023年1月泗阳康达医院收治的74例疑似糖尿病患者作为研究对象，检测入组患者糖化血红蛋白（glycosylated hemoglobin, HbA1c）以及血清C肽水平，以口服葡萄糖耐量试验（glucose tolerance test check, OGTT）为金标准，统计血清C肽联合糖化血红蛋白检测与单一项目检测的敏感性、特异度和诊断符合率。
结果74例疑似糖尿病患者根据葡萄糖耐量试验结果，确诊患者67例，确诊率为90.54%（67/74）；与血清C肽、HbA1c单一检测相比，血清C肽+HbA1c联合检测敏感度更高，差异有统计学意义（P<0.05）；血清C肽+HbA1c联合检测的特异度略高于血清C肽、HbA1c单一检测，但差异无统计学意义（P>0.05）；联合检测诊断符合率明显高于血清C 肽、HbA1c单项检测，差异有统计学意义（P<0.05）。
结论血清C肽与糖化血红蛋白是临床诊断糖尿病的重要参考指标，二者表达水平的变化有助于检测患者胰岛素分泌功能，评估疾病严重程度，两者联合检验灵敏性与特异度良好，有助于早期明确诊断，临床参考价值较高。
[关键词] 糖尿病；血清C肽；糖化血红蛋白；诊断价值[中图分类号] R446.1 [文献标识码] A [文章编号] 1672-4062（2023）07（b）-0085-04Analysis of the Value of the Diagnostic Application of Combined Serum C-peptide and Glycosylated Hemoglobin Testing in Patients with Diabetes MellitusNI Shengnan, CHEN Shao, CHEN YimingDepartment of Laboratory Medicine, Siyang Kangda Hospital, Suqian, Jiangsu Province, 223700 China[Abstract] Objective To explore the value of applying serum C-peptide combined with glycated hemoglobin test for the diagnosis of diabetic patients. Methods A total of 74 patients with suspected diabetes admitted to Siyang Kangda Hospital from January 2022 to January 2023 were selected as the research objects. The levels of glycosylated hemoglo‐bin (HbA1c) and serum C-peptide were detected. Oral glucose tolerance test (OGTT) was used as the gold standard. The sensitivity, specificity and diagnostic coincidence rate of serum C-peptide combined with glycosylated hemoglo‐bin detection and single item detection were statistically analyzed. Results According to the results of glucose toler‐ance test, 67 patients were diagnosed in 74 patients with suspected diabetes, and the diagnosis rate was 90.54% (67/ 74). Compared with the single detection of serum C-peptide and HbA1c, the sensitivity of combined detection of se‐rum C peptide and HbA1c was higher, and the difference was statistically significant (P<0.05). The specificity of com‐bined detection of serum C-peptide and HbA1c was slightly higher than that of single detection of serum C-peptide and HbA1c, but the difference was no statistically significant (P>0.05). The diagnostic coincidence rate of combined detection was significantly higher than that of single detection of serum C-peptide and HbA1c, and the difference was statistically significant (P<0.05). Conclusion Serum C-peptide and glycosylated hemoglobin are important reference indexes for clinical diagnosis of diabetes mellitus, and changes in the expression levels of the two can help to detect the insulin secretion function of patients and assess the severity of the disease. The sensitivity and specificity of the [作者简介]倪胜南（1991-），女，本科，主管检验师，研究方向为免疫学、分子生物学检验。
抗原恢复缓冲液（100X Tris-EDTA缓冲液，pH 9.0）说明书
Product nameAntigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)Tested applicationsSuitable for: IHC-P General notes Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed,paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies thatrequire Tris-EDTA buffer (pH 9.0) pretreatment.This product contains detergent for emulsification of the paraffin.1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.Protocol:Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plasticwrap, place in microwave and set high power to boil and then set low power to keep itboiling for 10 min. Let the sections cool in the microwave for at least 20min.Wash sections with hot tap water for 1 minute.Wash sections in buffer for 2x3 minutes.Continue with IHC protocolOther kits and reagents for IHCOther antigen retrieval buffers include: Citrate buffer pH 6.0 ab93678, EDTA buffer pH 8.0ab93680, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes .Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization,counterstaining, and mounting in the IHC kits and reagents guide .FormLiquid Storage instructionsStore at room temperature.Storage buffer pH: 8.7Constituents: 1.5% 2-butoxyethanol, 5% Sodium EDTA, 5% TrisBuffer 100x concentrated with detergent.Product datasheetAntigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)ab936843 Abreviews 22 References 3 ImagesOverview PropertiesThe Abpromise guaranteeImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retriev al Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)Image from M aniati et al., Cell Rep.;30(2):525-540.e7; doi: 10.1016/j.celrep.2019.12.034. Reproduced under the Creative Commons licensehttps:///licenses/by/4.0/Immunohistochemical for RUNX2 on murine omental metastasis Antigen retrieval: Tris-EDTA, pH;9 (ab93684), Blocking: Normal Goat Serum, Primary: Rabbit monoclonal anti-Runx2 [EPR14334] (ab192256), dilution 1:1000, Secondary: HRP anti-rabbitImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retriev al Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)ab93684 Antigen Retrieval Buffer 100X Tris-EDTA Buffer, pH 9ApplicationsOur Abpromise guarantee covers the use of ab93684 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews NotesIHC-P Use at an assay dependent dilution.ImagesImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retriev al Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling LINE-1 ORF1p with ab216324 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on spermatogonia of mouse testis (PMID: 24607009) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).Please note: A ll products are "FOR RESEA RCH USE ONLY. NOT FOR USE IN DIA GNOSTIC PROCEDURES"Our Abpromise to you: Quality guaranteed and expert technical supportReplacement or refund for products not performing as stated on the datasheetValid for 12 months from date of deliveryResponse to your inquiry within 24 hoursWe provide support in Chinese, English, French, German, Japanese and SpanishExtensive multi-media technical resources to help youWe investigate all quality concerns to ensure our products perform to the highest standardsIf the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit https:///abpromise or contact our technical team.Terms and conditionsGuarantee only valid for products bought direct from Abcam or one of our authorized distributors。
AF38469_SDS_MedChemExpress
Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:May-24-2017Print Date:May-24-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :AF38469Catalog No. :HY-12802CAS No. :1531634-31-71.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:AF⁻38469Formula:C15H11F3N2O3Molecular Weight:324.25CAS No. :1531634-31-74. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance White to off-white (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
大黄素检测
迪信泰检测平台
大黄素检测
大黄素（Emodin），又称为泻素，是从蓼科植物虎杖的干燥根茎和根中提取得到的一种化合物，存在于大黄、鼠尾草、决明子、虎杖等植物中。
具有抗肿瘤、抗炎、抗菌、清胆清肺、降血压的作用，可用于医疗、保健和日用化工品中。
迪信泰检测平台采用高效液相色谱(HPLC)法，结合蒸发光散射检测器(ELSD)或DAD 检测器，可高效、精准的检测样本中大黄素的含量变化。
此外，迪信泰检测平台还提供植物多酚检测服务，以满足您的不同需求。
HPLC测定大黄素样本要求：
1. 请确保样本量大于0.2g或者0.2mL。
周期：2~3周。
项目结束后迪信泰检测平台将会提供详细中英文双语技术报告，报告包括：
1. 实验步骤（中英文）。
2. 相关质谱参数（中英文）。
3. 质谱图片。
4. 原始数据。
5. 大黄素含量信息。
迪信泰检测平台可根据需求定制其他物质测定方案，具体可免费咨询技术支持。
AF38469_DataSheet_MedChemExpress
Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:AF38469 is a novel, selective, orally bioavailable Sortilin inhibitor with an IC 50 value of 330 nM.IC50 & Target: IC50:330 nM (Sortilin)[1]In Vitro: AF38469 showed no inhibition or stimulation of >50% at 10 μM in a standard selectivity panel of ca. 70 targets run at CEREP. Importantly AF38469 showed no activity against the NTR1 receptor. In addition AF38469 showed no activity against a selected panel of targets known to bind acidic molecules (d–Opioid, GPR40, PPARd, EP1, Angiotensin AT1, Endothelin ETA & B,MMP–12). AF38469 will serve as an important tool to further delineate the biology of Sortilin, and to facilitate evaluation of the therapeutic potential of this protein [1].PROTOCOL (Extracted from published papers and Only for reference)Enzyme in vitro assay [1]:Compound affinity was determined by measuring the displacement of 3H–neurotensin binding to hSortilin in SPA format. Total volume of 40 μl in 50 mM HEPES pH 7.4 assay buffer containing 100 mM NaCl, 2.0 mM CaCl2, 0.1% BSA and 0.1% Tween–20.Compound pre–incubation for 30 min at RT with 150 nM of 6his–Sortilin before 5 nM [3H]–Neurotensin and Ni chelate imaging beads (Perkin Elmer) were added, after 6 h plate was read on a ViewLux with 360 s exposure time. Dose–response evaluation of compounds was performed with 10 concentrations of drugs (covering 3 decades). IC50 values were calculated by nonlinear regression using the sigmoid concentration–response (variable slope) using Xlfit 4 (IDBS, UK). All values reported are average of at least 4 determination.References:[1]. Schroder TJ, et al. The identification of AF38469: an orally bioavailable inhibitor of the VPS10P family sorting receptor Sortilin. Bioorg Med Chem Lett.2014 Jan 1;24(1):177–80.Product Name:AF38469Cat. No.:HY-12802CAS No.:1531634-31-7Molecular Formula:C 15H 11F 3N 2O 3Molecular Weight:324.25Target:Others Pathway:Others Solubility:DMSO: ≥ 43 mg/mLCaution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@ Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
线粒体呼吸链复合体Ⅳ活性检测试剂盒说明书
线粒体呼吸链复合体可见分光光度法货号：BC0940规格：25T/24S、50T/48S产品组成：使用前请认真核对试剂体积与瓶内体积是否一致，有疑问请及时联系索莱宝工作人员。
试剂名称/规格25T50T保存条件提取液液体40 mL×1瓶液体80 mL×1瓶4℃保存试剂一液体21 mL×1瓶液体21 mL×2瓶4℃保存试剂二粉剂×1支粉剂×2支-20℃保存试剂三粉剂×1支粉剂×2支4℃保存溶液的配制：工作液的配制：临用前取试剂一、试剂二、试剂三，将试剂二和试剂三依次转移到试剂一中产品说明：线粒体复合体Ⅳ又称细胞色素C氧化酶，也是线粒体呼吸电子传递链主路和支路的共有成分，负责催化还原型细胞色素C的氧化，并最终把电子传递给氧生成水。
还原型细胞色素C在550nm有特征光吸收，线粒体复合体Ⅳ催化还原型细胞色素C生成氧化型细胞色素C，因此550nm光吸收下降速率能够反映线粒体复合体Ⅳ酶活性。
注意：实验之前建议选择2-3个预期差异大的样本做预实验。
如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。
需自备的仪器和用品：可见分光光度计、台式离心机、水浴锅、可调式移液器、1mL玻璃比色皿、研钵/匀浆器、冰和蒸馏水。
操作步骤：一、样本处理（可适当调整待测样本量，具体比例可以参考文献）1.称取约0.1g组织或收集500万细胞，加入1.0 mL提取液，用冰浴匀浆器或研钵匀浆。
4 ℃ 600g离心10min。
2.将上清液移至另一离心管中，4 ℃ 11000g离心15min。
3.上清液即胞浆提取物，可用于测定从线粒体泄漏的复合体Ⅳ（此步可选做，可以判断线粒体提取效果）。
4.在沉淀中加入400μL提取液，超声波破碎（功率20%，超声5秒，间隔10秒，重复15次），用于复合体Ⅳ酶活性测定，并且用于蛋白含量测定。
二、测定步骤1、可见分光光度计预热30min以上，调节波长至550nm，蒸馏水调零。
Adenosine Assay Kit 产品说明书
Product ManualAdenosine Assay KitCatalog NumberMET-5090 100 assays FOR RESEARCH USE ONLYNot for use in diagnostic proceduresIntroductionAdenosine is a purine nucleoside containing an adenine moiety attached to a ribose sugar molecule (ribofuranose) through a β-N9-glycosidic bond. Derivatives of adenosine perform an important role in energy transfer reactions (as adenosine triphosphate (ATP) and adenosine diphosphate (ADP)) as well as signal transduction (as cyclic adenosine monophosphate (cAMP)). Additionally, adenosine is a neuromodulator and is thought to promote sleep and suppress arousal. Adenosine also regulates blood flow to multiple organs through vasodilation. Adenosine is a byproduct of the enzymatic conversion of S-Adenosylhomocysteine (SAH) to homocysteine by Adenosylhomocystinease (AHCY).Adenosine causes a temporary block of the atrioventricular (AV) node in the heart, while also relaxing smooth muscle found inside the artery walls. Dilation of the "normal" segments of arteries allows physicians to use adenosine to test for blockages in the coronary arteries, by exaggerating the difference between the normal and abnormal segments. In people suspected of having a supraventricular tachycardia (SVT), adenosine can be used to help identify the problem. Certain SVTs can be successfully stopped with adenosine. In addition, atrial tachycardia can sometimes be terminated with adenosine. Finally, adenosine is used in combination with thallous (thallium) chloride TI 201 or Tc99m myocardial perfusion scintigraphy (nuclear stress test for heart attack risk) in people who are unable to undergo sufficient stress testing with exercise.Ce ll Biolabs’ Adenosine Assay Kit is a simple fluorometric assay that measures the amount of total adenosine present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, adenosine standards, and unknown samples. Sample adenosine concentrations are determined by comparison with a known adenosine standard. The kit has a detection sensitivity limit of 1.56 µM adenosine.*Note: Each sample replicate requires 2 assays, one treated with adenosine deaminase (+ADA) and one without (-ADA). Adenosine is calculated from the difference in RFU readings from the 2 wells. Assay PrincipleCell Biolabs’ Adenosine Assay Kit measures total adenosine within biological samples. Adenosine is converted into inosine by adenosine deaminase (ADA). Then inosine is converted into hypoxanthine by purine nucleoside phosphorylase (PNP). Finally, hypoxanthine is converted to xanthine and hydrogen peroxide by xanthine oxidase (XO). The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of adenosine standard within the 96-well microtiter plate format. Samples and standards are incubated for 15 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).Figure 1.Adenosine Assay Principle.Related Products1.MET-5092: Inosine Assay Kit2.MET-5151: S-Adenosylhomocysteine (SAH) ELISA Kit3.MET-5152: S-Adenosylmethionine (SAM) ELISA KitKit Components1.Adenosine Standard (Part No. 50901C): One 50 µL tube at 10 mM.2.10X Assay Buffer (Part No. 268002): One 25 mL bottle of 500 mM sodium phosphate pH 7.4.3.Fluorometric Probe (Part No. 50231C): One 50 µL tube in DMSO.4.HRP (Part No. 234402-T): One 10 μL t ube of a 100 U/mL solution in glycerol.5.Adenosine Deaminase (Part No. 50902C): One 10 µL tube at 1000 U/mL.Note: One unit is defined as the amount of enzyme that will deaminate 1.0 μmole of adenosine to inosine per min. at pH 7.5 at 25°C.6.Purine Nucleoside Phosphorylase (Part No. 50903D): One 500 µL tube at 18.9 U/mL.Note: One unit is defined as the amount of enzyme that will cause the phosphorolysis of 1.0 μmole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.4 at 25°C.7.Xanthine Oxidase (Part No. 50904D): one 100 µL tube at 2.5 U/mL.Note: One unit is defined as the amount of enzyme that will convert 1.0 μmol e of xanthine to uric acid per min at pH 7.5 at 25°C. About 50% of the activity is obtained with hypoxanthine as substrate.Materials Not Supplied1.Phosphate Buffered Saline (PBS)2.10 μL to 1000 μL adjustable single channel micropipettes with disposable ti ps3.50 μL to 300 μL adjustable multichannel micropipette with disposable tips4.Standard 96-well fluorescence black microtiter plate and/or black cell culture microplate5.Multichannel micropipette reservoir6.Fluorescence microplate reader capable of reading excitation in the 530-570 nm range and emissionin the 590-600 nm range.StorageUpon receipt, store the 10X Assay Buffer at room temperature and store the rest of the kit at -20ºC. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.Note: After thawing any of the three enzymes for the first time, make smaller aliquots and store at-20°C.Preparation of Reagents•1X Assay Buffer: Dilute the stock 10X Assay Buffer 1:10 with deionized water for a 1X solution.Stir or vortex to homogeneity. Store at room temperature.•Reaction Mix: Prepare a Reaction Mix by diluting the Fluorometric Probe 1:100, HRP 1:500, Adenosine Deaminase 1:500, Purine Nucleoside Phosphorylase 1:10, and Xanthine Oxidase 1:50 in 1X Assay Buffer. For example, add 10 μL Fluorometric Probe stock solution, 2 μL HRP stock solution, 2 µL of Adenosine Deaminase, 100 µL of Purine Nucleoside Phosphorylase, and 20 μL of Xanthine Oxidase to 866 µL of 1X Assay Buffer for a total of 1 mL. This Reaction Mix volume is enough for 20 assays. The Reaction Mix is stable for 1 day at 4ºC.Note: Prepare only enough for immediate use by scaling the above example proportionally. •Control Mix: Prepare a Reaction Mix (without adenosine deaminase) by diluting the Fluorometric Probe 1:100, HRP 1:500, Purine Nucleoside Phosphorylase 1:10, and Xanthine Oxidase 1:50 in 1X Assay Buffer. For example, add 10 μL Fluorometric Probe stock solution, 2 μL HRP stocksolution, 100 µL of Purine Nucleoside Phosphorylase, and 20 μL of Xanthine Oxidase to 868 µL of 1X Assay Buffer for a total of 1 mL. This Control Mix volume is enough for 20 assays. TheControl Mix is stable for 1 day at 4ºC.Note: Prepare only enough for immediate use by scaling the above example proportionally.Preparation of Samples• Cell culture supernatants: Cell culture media containing adenosine, inosine, xanthine, and hypoxanthine should be avoided. To remove insoluble particles, centrifuge at 10,000 rpm for 5 min. The cell conditioned media may be assayed directly or diluted as necessary in PBS.Note: Maintain pH between 7 and 8 for optimal working conditions as the Fluorometric Probe is unstable at high pH (>8.5).• Tissue lysates: Sonicate or homogenize tissue sample in PBS and centrifuge at 10,000 x g for 10 minutes at 4°C. The supernatant may be assayed directly or diluted as necessary in PBS.• Cell lysates: Resuspend cells at 1-2 x 106 cells/mL in PBS. Homogenize or sonicate the cells on ice. Centrifuge to remove debris. Cell lysates may be assayed undiluted or diluted as necessary in PBS.• Serum, plasma or urine: To remove insoluble particles, centrifuge at 10,000 rpm for 5 min. The supernatant may be assayed directly or diluted as necessary in PBS.Notes:• All samples should be assayed immediately or stored at -80°C for up to 1-2 months. Run proper controls as necessary. Optimal experimental conditions for samples must be determined by the investigator. Always run a standard curve with samples.• Samples with NADH concentrati ons above 10 μM and glu tathione concentrations above 50 μM will oxidize the Fluorometric Probe and could result in erroneous readings. To minimize this interference, it is recommended that superoxide dismutase (SOD) be added to the reaction at a final concentration of 40 U/mL (Votyakova and Reynolds, Ref. 2).• Avoid samples containing DTT or β-mercaptoethanol since the Fluorometric Probe is not stable in the presence of thiols (above 10 μM).Preparation of Standard CurvePrepare fresh Adenosine standards according to Table 1 below.Table 1. Preparation of Adenosine Standards.Assay Protocol1. Prepare and mix all reagents thoroughly before use. Each sample, including unknowns and standards, should be assayed in duplicate or triplicate. Standard Tubes 10 mM Adenosine Solution(µL)PBS (µL) Adenosine (µM) 1 5495 100 2 250 of Tube #1250 50 3 250 of Tube #2250 25 4 250 of Tube #3250 12.5 5 250 of Tube #4250 6.25 6 250 of Tube #5250 3.13 7 250 of Tube #6250 1.56 8 0250 0Note: Each sample replicate requires two paired wells, one to be treated with Adenosine Deaminase (+ADA) and one without the enzyme (-ADA) to measure endogenous background.2.Add 50 μL of each standard into wells of a black microtiter plate suitable for a f luorescence platereader.3.Add 50 μL of each unknown sample to each of two separate wells.4.Add 50 μL of Reaction Mix to all standard wells and one half of the paired sample wells.5.Add 50 μL of Control Mix to the remaining paired sample wells.6.Mix the well contents thoroughly and incubate for 15 minutes at room temperature protected fromlight.Note: This assay is continuous (not terminated) and therefore may be measured at multiple time points to follow the reaction kinetics.7.Read the plate with a fluorescence microplate reader equipped for excitation in the 530-570 nmrange and for emission in the 590-600 nm range.Example of ResultsThe following figure demonstrates typical Adenosine Assay Kit results. One should use the data below for reference only. This data should not be used to interpret or calculate actual sample results.Figure 2: Adenosine Standard Curve.Calculation of Results1.Determine the average Relative Fluorescence Unit (RFU) values for each sample, control, andstandard.2.Subtract the average zero standard value from itself and all standard values.3.Graph the standard curve (see Figure 2).4.Subtract the sample well values without Adenosine Deaminase (-ADA) from the sample well valuescontaining Adenosine Deaminase (+ADA) to obtain the difference. The fluorescence difference is due to the Adenosine Deaminase activity.Net RFU = (RFU+ADA)- (RFU-ADA)5. Compare the net RFU of each sample to the standard curve to determine and extrapolate thequantity of Adenosine present in the sample. Only use values within the range of the standard curve.References1.Sato, A (A2005). Am. J. of Physiol.Heart Circ. Physiol.288: H1633–402.Votyakova TV, and Reynolds IJ (2001) Neurochem. 79:266.3.Costa, F and Biaggioni, I (1998). Hypertension. 31: 1061–44.Morgan, JM; McCormack, DG; Griffiths, MJ; Morgan, CJ; Barnes, PJ and Evans, TW (1991).Circulation. 84: 1145–9.5.Mitchell J and Lazarenko G (2008). CJEM. 10: 572–3.6.O'Keefe, JH; Bateman, TM; Silverstri, R and Barnhart C. (1992). Am Heart J.124: 614–21. Recent Product Citations1.Ndzie Noah, M.L. et al. (2023). Estrogen downregulates CD73/adenosine axis hyperactivity viaadaptive modulation PI3K/Akt signaling to prevent myocarditis and arrhythmias during chronic catecholamines stress. Cell Commun Signal. 21(1):41. doi: 10.1186/s12964-023-01052-0.2.Fu, Z. et al. (2023). Proteolytic regulation of CD73 by TRIM21 orchestrates tumor immunogenicity.Sci Adv. 9(1):eadd6626. doi: 10.1126/sciadv.add6626.3.Pepponi, R. et al. (2022). Repurposing Dipyridamole in Niemann Pick Type C Disease: A Proof-of-Concept Study. Int J Mol Sci. 23(7):3456. doi: 10.3390/ijms23073456.4.Kim, G.T. et al. (2022). PLAG co-treatment increases the anticancer effect of Adriamycin andcyclophosphamide in a triple-negative breast cancer xenograft mouse model. Biochem Biophys Res Commun. 619:110-116. doi: 10.1016/j.bbrc.2022.06.051.5.Tsai, C.H. et al. (2022). Carbohydrate metabolism is a determinant for the host specificity ofbaculovirus infections. iScience. doi: 10.1016/j.isci.2021.103648.6.Xue, G. et al. (2021). Elimination of acquired resistance to PD-1 blockade via the concurrentdepletion of tumour cells and immunosuppressive cells. Nat Biomed Eng. 5(11):1306-1319. doi:10.1038/s41551-021-00799-6.7.Murphy, D.A. et al. (2021). Reversing Hypoxia with PLGA-Encapsulated Manganese DioxideNanoparticles Improves Natural Killer Cell Response to Tumor Spheroids. Mol Pharm. doi:10.1021/acs.molpharmaceut.1c00085.8.Badimon, A. et al. (2020). Negative feedback control of neuronal activity by microglia. Nature. doi:10.1038/s41586-020-2777-8.9.Huang, L. et al. (2020). Inhibition of A2B Adenosine Receptor Attenuates Intestinal Injury in a RatModel of Necrotizing Enterocolitis. Mediators Inflamm. doi: 10.1155/2020/1562973.10.Basu, M. et al. (2020). Increased host ATP efflux and its conversion to extracellular adenosine iscrucial for establishing Leishmania infection. J Cell Sci. pii: jcs.239939. doi: 10.1242/jcs.239939.11.Duan, L. et al. (2020). Late Protective Effect of Netrin-1 in the Murine AcetaminophenHepatotoxicity Model. Toxicol Sci. pii: kfaa041. doi: 10.1093/toxsci/kfaa041.12.Chang, Y. et al. (2020). Snellenius manilae bracovirus suppresses the host immune system byregulating extracellular adenosine levels in Spodoptera litura. Sci Rep. 10(1):2096. doi:10.1038/s41598-020-58375-y.13.Ali, R.A. et al. (2019). Adenosine receptor agonism protects against NETosis and thrombosis inantiphospholipid syndrome. Nat Commun. 10(1):1916. doi: 10.1038/s41467-019-09801-x. WarrantyThese products are warranted to perform as described in their labeling and in Cell Biolabs literature when used in accordance with their instructions. THERE ARE NO WARRANTIES THAT EXTEND BEYOND THIS EXPRESSED WARRANTY AND CELL BIOLABS DISCLAIMS ANY IMPLIED WARRANTY OF MERCHANTABILITY OR WARRANTY OF F ITNESS FOR PARTICULAR PURPOSE. CELL BIOLABS’s sole obligation and purchaser’s exclusive remedy for breach of this warranty shall be, at the option of CELL BIOLABS, to repair or replace the products. In no event shall CELL BIOLABS be liable for any proximate, incidental or consequential damages in connection with the products.Contact InformationCell Biolabs, Inc.7758 Arjons DriveSan Diego, CA 92126Worldwide: +1 858 271-6500USA Toll-Free: 1-888-CBL-0505E-mail: ********************©2017-2023: Cell Biolabs, Inc. - All rights reserved. No part of these works may be reproduced in any form without permissions in writing.。
天门冬氨酸氨基转移酶(AST)测定试剂盒(天门冬氨酸底物法)产品技术要求国赛生物
天门冬氨酸氨基转移酶（AST）测定试剂盒
性能指标
1.性能指标
1.1外观
试剂 1 应为清澈透明液体；
试剂 2 应为清澈透明液体。
1.2装量
每瓶试剂的装量应不小于标示装量。
1.3试剂空白
1.3.1试剂空白吸光度
在 340nm 波长、1cm 光径下，试剂空白吸光度应≥1.0。
1.3.2试剂空白吸光度变化率
试剂空白吸光度变化率应≤0.004/min。
1.4分析灵敏度
单位浓度吸光度变化率应≥0.0001/min。
1.5准确度
相对偏差应不超过±10.0%。
1.6精密度
1.6.1批内精密度
批内变异系数CV≤5.0%。
1.6.2批间精密度
批间相对极差R≤10.0%。
1.7线性区间
在[5.0,1000.0]U/L 区间内，线性相关系数r≥0.9900。
[5.0,50.0]U/L 区间内，绝对偏差不超过±5.0U/L；(50.0，1000.0]U/L 区间内，相对偏差不超过±10.0%。