塞来昔布抑制人肺腺癌细胞的增殖和表皮生长因子受体的表达

塞来昔布抑制人肺腺癌细胞的增殖和表皮生长因子受体的表达王亚丽;牟晓燕;魏启宏;吴峰;白小燕;刘庆亮
【期刊名称】《中国肿瘤临床》
【年(卷),期】2011(38)12
【摘要】Objective: To explore the effects of celecoxib on the proliferation and expression of epidermal growth factor receptor ( EGFR ) inhibitor in lung cancer A549 cells at different concentrations and different time points.Methods: A549 cells were cultured in RPMI-1640 and divided into five groups: normal control group; 12.5 μmol/L celecoxib group; 25 μmol/L celecoxib group; 50 μmol/L celecoxib group; and 75 μmol/L celeco xib group.The cells were treated with celecoxib in different doses ( 12.5, 25, 50, and 75 μmol/ L ) and for different periods ( 24, 48, and 72 hours ).Cell inhibition rate was detected by methyl thiazolyl tetrazolium ( MTT ).The apoptosis rate was measured using the Annexin V/PI and Hoechst 33258 staining method.The cell cycle was detected by flow cytometry, and the expression of EGFR mRNA was determined through real-time reverse transcriptase polymerase chain reaction.Results: Celecoxib induced a dose- and time-dependent growth inhibition in the intervention groups, as shown by MTT assay.Higher apoptosis rates ( P ＜ 0.01 ) and G0/G1 stage cell ratios ( P ＜ 0.01 ), and lower rates of S stage cell proportion ( P ＜
0.01 ), and EGFR mRNA expression ( P ＜ 0.01 ) were observed in the celecoxib treatment groups compared with the normal control
group.Conclusion: Celecoxib significantly inhibits the growth of A549 cell, possibly by promoting apoptosis, G0-G1 arrest, and downregulation of EGFR mRNA expression.Celecoxib has potential application in lung cancer treatment.Our study provides a new therapeutic use for celecoxib combined with EGFR inhibitors.%目的:探讨不同浓度的环氧化酶-2抑制剂塞来
昔布(Celecoxib)在不同时间点对肺腺癌A549细胞株增殖和表皮生长因子受体(EGFR)表达的影响.方法:人肺腺癌A549细胞株培养于含10%胎牛血清
RPMI1640培养基中.实验细胞分组如下:A组,正常对照;B组,Celecoxib(12.5
μmol/L);C组Celecoxib(25 μmol/L);D组Celecoxib(50 μmol/L),E组Celecoxib(75 μmol/L),均以RPMI1640培养液配置.使用不同浓度塞来昔布(12.5、25、50和75 μmol/L)处理肺癌A549细胞24、48、72 h后,四甲基偶氮唑盐比
色法(MTT)测定细胞增殖抑制率;AnnexinⅤ/PI染色法与Hoechst33258染色法检测细胞凋亡率;流式细胞仪检测药物作用周期;Real-time RT-PCR法检测EGFR mRNA的表达情况.结果:塞来昔布明显抑制了A549细胞的生长,呈时间、剂量依
赖性.塞来昔布组细胞凋亡率明显高于正常对照组(P<0.01),且呈剂量依赖性,S期细胞比例明显减少(P<0.01),G0/G1期细胞比例明显增加(P<0.01),提示塞来昔布能将大多数A549细胞阻滞于G0/G1期.塞来昔布组细胞EGFR mRNA表达明显减弱(P<0.05),且呈浓度依赖性,各浓度间差异具有统计学意义(P<0.05).结论:塞来昔布
显著抑制A549细胞生长,可能机制是通过促进凋亡、增强G0/G1期阻滞、下调细胞中EGFR mRNA的表达,从而为应用塞来昔布治疗肺腺癌,以及塞来昔布和表皮生长因子受体抑制剂联用提供了一定的实验依据.
【总页数】4页(P708-711)
【作者】王亚丽;牟晓燕;魏启宏;吴峰;白小燕;刘庆亮
【作者单位】扬州市第一人民医院呼吸科,江苏省扬州市,225002;山东大学附属省立医院;扬州市第一人民医院呼吸科,江苏省扬州市,225002;扬州市第一人民医院呼吸科,江苏省扬州市,225002;山东大学附属省立医院;山东大学附属省立医院
【正文语种】中文
【相关文献】
1.环氧化酶-2抑制剂塞来昔布联合顺铂对A549人肺腺癌细胞株增殖影响的体外实验研究 [J], 熊建萍;陶庆松;张凌;项小军
2.人源噬菌体抗体对Peroxiredoxin I高表达肺腺癌细胞增殖的抑制作用 [J], 罗弋;庞华;李淑杰;曹辉;李少林;樊春波
3.联合抑制人肺腺癌细胞的MCT1基因及NHE1基因表达对其增殖生长的影响[J], 张桂芝;黄桂君;厉为良;吴国明;钱桂生
4.塞来昔布抑制人结肠癌细胞 COLO205增殖及对 COX -2、MMP -9mRNA 表达影响的体外实验研究 [J], 郭东来;李鹏;韩振魁;斯坎德尔·努尔买买提
5.抑制人肺腺癌细胞MCT1基因的表达对其增殖、凋亡的影响 [J], 张桂芝;黄桂君;成党校;郭先健;钱桂生
因版权原因，仅展示原文概要，查看原文内容请购买。