结核分枝杆菌广泛耐药株与耐多药株菌体蛋白质组学比较研究

结核分枝杆菌广泛耐药株与耐多药株菌体蛋白质组学比较研究王盛兰;陈玲;张建勇;宗兆靖;王宝林;李娜娜;王建华;张泓
【期刊名称】《中国人兽共患病学报》
【年(卷),期】2011(027)009
【摘要】The aim was to compare and analyze the protein expression profiles of extensively drug-resistant (XDR) and multidrug-resistant(MDR) tuberculosis isolates, and identify differentially expressed proteins related to XDR-TB. Total proteins extracted from clinical isolates of XDR-TB and MDR-TB as well as the standard mycobacterium tuberculosis H37Rv were analyzed with two-dimensional electrophoresis (2-DE). The Progenesis SameSpots program (Nonlinear Dynamics Limited, U-nited Kingdom) was used for analyzing the 2-DE images to identify differentially expressed proteins in XDR-TB as compared to MDR-TB and the standard strain, and some selected protein spots were used for the MALDI-MS peptide mass fingerprinting. The results of this study showed that protein expression profile of XDR-TB was significantly different from those of MDR-TB and the H37Rv standard strain. There were twenty-eight differentially expressed protein spots in XDR-TB. Fifteen proteins differentially expressed in XDR-TB were identified by MALDI-MS peptide mass fingerprinting, Protein expression profile of XDR-TB is different from those of MDR-TB and the
H37Rv standard strain, and the differentially expressed proteins could be used as potential target proteins for future investigations to study their
functions in XDR-TB.%目的比较和分析结核分枝杆菌广泛耐药(XDR)与耐多药(MDR)菌株在菌体蛋白质表达水平上的差异,并寻找与XDR相关的蛋白质点.方法利用双向凝胶电泳分离XDR、MDR菌株以及H37Rv标准菌株菌体蛋白质,通过计算机图像分析软件分析各菌株之间在菌体蛋白质表达水平上的差异性,并进行质谱分析.结果 XDR与MDR以及H37Rv菌株之间在菌体蛋白质表达水平上存在明显差异；与XDR相关的28个差异(新增、缺失、上调、下调)表达的蛋白质点中,质谱分析鉴定出5个相关的蛋白质点.结论结核分枝杆菌XDR与MDR菌株在菌体蛋白质表达水平上存在明显差异,为进一步研究XDR菌株耐药机理奠定了基础.【总页数】5页(P820-824)
【作者】王盛兰;陈玲;张建勇;宗兆靖;王宝林;李娜娜;王建华;张泓
【作者单位】遵义医学院附属医院呼吸内科、呼吸医学研究室,遵义563003;遵义医学院附属医院呼吸内科、呼吸医学研究室,遵义563003;遵义医学院附属医院呼吸内科、呼吸医学研究室,遵义563003;遵义医学院附属医院呼吸内科、呼吸医学研究室,遵义563003;遵义医学院附属医院呼吸内科、呼吸医学研究室,遵义563003;遵义医学院附属医院呼吸内科、呼吸医学研究室,遵义563003;遵义医学院附属医院呼吸内科、呼吸医学研究室,遵义563003;遵义医学院附属医院呼吸内科、呼吸医学研究室,遵义563003
【正文语种】中文
【中图分类】R378.91
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