瑞舒伐他汀联合脐血间充质干细胞移植改善急性心肌梗死后心功能

瑞舒伐他汀联合脐血间充质干细胞移植改善急性心肌梗死后心
功能
王艳丽;李金峰;王艳萍;高明
【期刊名称】《中国组织工程研究》
【年(卷),期】2016(020)019
【摘要】BACKGROUND:In recent years, it has been a hot topic that stem cel transplantation is used to improve cardiac insufficiency after acute myocardial infarction by inducing regeneration of cardiomyocytes in the infarction regions. OBJECTIVE:To observe the effect of rosuvastatin combined with umbilical cord blood mesenchymal stem cel s transplantation on rat cardiac function after acute myocardial infarction. METHODS:Forty-five Sprague-Dawley rats were enrol ed to prepare myocardial infarction models by ligaturing the left anterior descending coronary artery. Then they were equivalently divided into model group, transplantation group and combination group. At 7 days after modeling, rats in the combination group were given injection of 300μL umbilical cord blood mesenchymal stem cel s (15.0×108) via the tail vein and by gavage once a day for 28 days with 1 mg/kg rosuvastatin;rats in the transplantation group and model gr oup were injected with 300μL umbilical cord blood mesenchymal stem cel suspension through the tail veins or the same amount of LG-DMEM medium, respectively, fol owed by intragastrical administration of the same amount normal saline. At 5 weeks
after modeling, indexes of cardiac function, level of plasma Lp-PLA2 and heat shock protein 70 in the infarction regions were detected by color Doppler ultrasound, enzyme-linked immunosorbent assay and western
blot assay, respectively. In addition, pathological changes of myocardial tissues were observed using hematoxylin-eosin staining. RESULTS AND CONCLUSION:Left ventricular ejection fraction and left ventricular end-systolic pressure were significantly higher in the combination group than
in the transplantation group as wel as higher in the transplantation group than the model group (P<0.05);compared with the transplantation group, left ventricular end-diastolic pressure was significantly decreased in the combination group, but significantly increased in the model group
(P<0.05);the number of cardiomyocytes in the infarction regions was significantly higher in the combination group than the other groups. Additional y, expression of heat shock protein 70 in the infarction regions was significantly increased in the combination group (P<0.05). To conclude, rosuvastatin combined with umbilical cord blood mesenchymal stem cel transplantation can significantly improve rat cardiac function after myocardial infarction.%背景：近年来通过干细胞移植诱导梗死区心肌细胞的再
生用以治疗急性心肌梗死后心功能不全，成为较为热议的一种新的治疗手段。

<br> 目的：观察瑞舒伐他汀联合脐血间充质干细胞移植对大鼠急性心肌梗死后心功能的改善作用。

<br> 方法：选取45只SD大鼠采用结扎左冠状动脉前降支方法
制备心肌梗死模型，随机分为模型组、脐血间充质干细胞移植组和联合组，每组
15只。

大鼠冠脉结扎后第7天，脐血间充质干细胞移植组及联合组经尾静脉注射
脐血间充质干细胞悬液300μL(15.0×108个细胞)，模型组注射相同剂量LG-
DMEM培养液，联合组给予瑞舒伐他汀1 mg/kg灌胃×28 d(从术后第1天开始
灌胃，1次/d)，脐血间充质干细胞移植组及模型组同步灌胃相同剂量的生理盐水。

造模后5周(细胞移植4周)采用彩色多普勒超声仪检测心功能改善情况，酶联免疫吸附法检测血浆Lp-PLA2水平，苏木精-伊红染色观察心肌组织病理变化；Western blot方法检测心肌梗死区热休克蛋白70表达。

<br> 结果与结论：①联合组大鼠的左心室射血分数、左心室收缩末压明显高于脐血间充质干细胞移植组(P<0.05)，脐血间充质干细胞移植组明显高于模型组(P<0.05)；联合组大鼠的
左心室舒张末压明显低于脐血间充质干细胞移植组(P <0.05)，脐血间充质干细胞
移植组明显低于模型组(P <0.05)；②联合组Lp-PLA2水平明显低于脐血间充质干细胞移植组(P<0.05)，脐血间充质干细胞移植组明显低于模型组(P<0.05)；③联
合组梗死区的心肌细胞数量较模型组、脐血间充质干细胞移植组明显增加；④心肌梗死区HSP70蛋白表达在联合组明显升高，各组间的表达差异有显著性意义(P
<0.05)；⑤结果表明，瑞舒伐他汀联合脐血间充质干细胞移植干预后能确切改善
心肌梗死大鼠心功能。

【总页数】7页(P2796-2802)
【作者】王艳丽;李金峰;王艳萍;高明
【作者单位】唐山市开滦总医院心内科，河北省唐山市 063000;唐山市开滦总医
院心内科，河北省唐山市 063000;唐山市遵化市人民医院，河北省唐山市 064200;唐山市开滦总医院心内科，河北省唐山市 063000
【正文语种】中文
【中图分类】R394.2
【相关文献】
1.HGF基因转染脂肪来源干细胞移植改善兔急性心肌梗死后心功能的研究 [J], 裴艳;徐亚伟;侯磊;沈建颖
2.瑞舒伐他汀联合曲美他嗪治疗急性心肌梗死后心绞痛患者心功能、血脂水平及血液流变学水平的影响 [J], 丁友芳;祝锐
3.瑞舒伐他汀联合脐血间充质干细胞移植改善急性心肌梗死后心功能 [J], 王艳丽;李金峰;王艳萍;高明;
4.尼可地尔联合曲美他嗪对改善老年急性心肌梗死急性PCI术后心功能的作用 [J], 韩孝宇;安建立;王文广;杨红梅;张晶;王庆胜;代玉涵;刘晓媛
5.瑞舒伐他汀对急性心肌梗死患者PCI术后心功能、血管内皮功能及炎症因子的影响 [J], 贺正波;束晨
因版权原因，仅展示原文概要，查看原文内容请购买。