QPRT、miR-26b-5p在乳腺癌中表达及其与患者预后的关系
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potential targets for clinical treatment of breast cancer.
【 Ｋｅｙ ｗｏｒｄｓ】 Breast cancer; Quinolinic acid phosphoribosyl transferase; microRNA￣26b￣5p; Prognosis; Correlation
Ｓｃｉｅｎｃｅ ａｎｄ Ｔｅｃｈｎｏｌｏｇｙ Ｐｒｏｊｅｃｔ (１８￣ＨＤＷＳ￣０２３)
【 Ａｂｓｔｒａｃｔ】 Ｏｂｊｅｃｔｉｖｅ To analyze the expression of quinolinic acid phosphoribosyl transferase (QPRT) and mi￣
croRNA￣26b￣5p (miR￣26b￣5p) in breast cancer and their relationship with the prognosis of patients.Ｍｅｔｈｏｄｓ From March
2015 to October 2017, 89 female patients undergoing modified radical mastectomy in the Breast Department of Guangzhou
miR￣26b￣5p expression are risk factors affecting postoperative recurrence and metastasis of breast cancer patients [ OR
(95% CI) = 2.381 (1.394 － 4.066), 2.317 (1.272 － 4.221 ), 2.449 (1.243 － 4.825 ), 2.604 (1.501 － 4.517 ), 3.007 (1.6ｓｉｏｎ The up￣regulation of QPRT expression in breast cancer tissues and the down￣regulation of miR￣26b￣
5p expression are risk factors affecting postoperative recurrence and metastasis of breast cancer patients, and may provide
２. ６０４(１. ５０１ ~ ４. ５１７) 、３. ００７(１. ６２５ ~ ５. ５６４) ] ꎮ 结论 乳腺癌组织中 ＱＰＲＴ 表达上调ꎬｍｉＲ￣２６ｂ￣５ｐ 表达下调ꎬ是影响
乳腺癌患者术后发生复发转移的危险因素ꎬ可能为临床治疗乳腺癌提供潜在靶点ꎮ
【 关键词】 乳腺癌ꎻ喹啉酸磷酸核糖基转移酶ꎻ微小 ＲＮＡ￣２６ｂ￣５ｐꎻ预后ꎻ相关性
level and postoperative recurrence and metastasis was analyzed. Factor analysis of the influencing factors of recurrence and
metastasis of breast cancer patients.Ｒｅｓｕｌｔｓ The positive expression rate of QPRT in the observation group was significant￣
【 中图分类号】 Ｒ７３７. ９ 【 文献标识码】 Ａ
Ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＱＰＲＴ ａｎｄ ｍｉＲ￣２６ｂ￣５ｐ ｉｎ ｂｒｅａｓｔ ｃａｎｃｅｒ ａｎｄ ｔｈｅｉｒ ｒｅｌａｔｉｏｎｓｈｉｐ ｗｉｔｈ ｔｈｅ ｐｒｏｇｎｏｓｉｓ ｏｆ ｐａｔｉｅｎｔｓ Ｙｕａｎ
【 摘 要】 目的 分析喹啉酸磷酸核糖基转移酶( ＱＰＲＴ) 、微小 ＲＮＡ￣２６ｂ￣５ｐ( ｍｉＲ￣２６ｂ￣５ｐ) 在乳腺癌中表达及其
与患者预后的关系ꎮ 方法 选取 ２０１５ 年 ３ 月—２０１７ 年 １０ 月于广州市中西医结合医院行乳腺癌( 经病理组织检查均
为乳腺浸润性导管癌) 改良根治术女性患者 ８９ 例为研究对象ꎬ获取乳腺癌组织( 观察组) 和癌旁正常乳腺组织( 对照
pression was negatively correlated (r/P = － 0.343/0.000 ). The expression levels of QPRT and miR￣26b￣5p were related to
疑难病杂志 ２０２１ 年 ５ 月第 ２０ 卷第 ５ 期 Ｃｈｉｎ Ｊ Ｄｉｆｆｉｃ ａｎｄ Ｃｏｍｐｌ ＣａｓꎬＭａｙ ２０２１ꎬＶｏｌ. ２０ Ｎｏ. ５
Hospital of Integrated Traditional Chinese and Western Medicine (all were invasive ductal carcinoma of the breast after path￣
ological examination) were selected as the research objects, and breast cancer tissues were obtained. (Observation group)
and normal breast tissue adjacent to cancer (control group), immunohistochemical method was used to detect QPRT expres￣
sion, in situ hybridization was used to detect miR￣26b￣5p expression level, and the relationship between QPRT, miR￣26b￣5p
tients with high QPRT expression was significantly higher than that of patients with low QPRT expression (65.38% vs.
29.73% , χ2 /P = 13.260/0.000 ), the postoperative recurrence and metastasis rate of patients with low miR￣26b￣5p expres￣
ly higher than that of the control group, and the positive expression rate of miR￣26b￣5p was significantly lower than that of
the control group (χ2 /P = 54.395/0.000, 23.448/0.000); QPRT and miR￣26b￣5p were found in breast cancer tissues The ex￣
乳腺癌组织中表达呈负相关( ｒ / Ｐ ＝ － ０. ３４３ / ０. ０００) ꎻＱＰＲＴ、ｍｉＲ￣２６ｂ￣５ｐ 表达水平与患者 ＴＮＭ 分期、肿瘤分化程度、淋
巴结转移有关( ＱＰＲＴ:χ２ / Ｐ ＝ ５. ３２６ / ０. ０２１、４. ９９１ / ０. ０２５、６. ７４０ / ０. ００９ꎬｍｉＲ￣２６ｂ￣５ｐ:χ２ / Ｐ ＝ ５. ９０２ / ０. ０１５、４. ９４６ / ０. ０２６、
Ｎａｎｇｕｉ ∗ ꎬ Ｌｕｏ Ｘｕｅｐｉｎｇꎬ Ｈｕ Ｙｕｐｉｎｇꎬ Ｙａｏ Ｂｉｎꎬ Ｈｕａｎｇ Ｗｅｉｌｉｎｇ.
∗
Ｄｅｐａｒｔｍｅｎｔ ｏｆ Ｍａｍｍａｒｙ Ｇｌａｎｄꎬ Ｇｕａｎｇｚｈｏｕ Ｉｎｔｅｇｒａｔｅｄ Ｔｒａ￣
ｄｉｔｉｏｎａｌ Ｃｈｉｎｅｓｅ ａｎｄ Ｗｅｓｔｅｒｎ Ｍｅｄｉｃｉｎｅ Ｈｏｓｐｉｔａｌꎬ Ｇｕａｎｇｄｏｎｇ ＰｒｏｖｉｎｃｅꎬＧｕａｎｇｚｈｏｕ ５１０８００ꎬＣｈｉｎａ
与患者预后的关系
袁南贵ꎬ罗雪平ꎬ胡玉萍ꎬ姚斌ꎬ黄伟玲
基金项目: 广东省医学科学技术研究基金项目( Ａ２０１８０７４) ꎻ广州市花都区科技计划项目(１８￣ＨＤＷＳ￣０２３)
作者单位: ５１０８００ 广州市中西医结合医院乳腺科( 袁南贵、胡玉萍、姚斌、黄伟玲) ꎬ检验科( 罗雪平)
通信作者: 胡玉萍ꎬＥ￣ｍａｉｌ:Ｈｎｘｙｋｊｙｈ￣ｂｘ＠ １６３. ｃｏｍ
４７７
the patient’ s TNM stage, tumor differentiation, and lymph node metastasis (QPRT: χ2 /P = 5.326/0.021, 4.991/0.025, 6.740/
0.009, miR￣26b￣5p: χ2 /P = 5.902/0.015, 4.946/0.026, 7.949/0.005 ); the postoperative recurrence and metastasis rate of pa￣
sion was significantly higher than that of patients with high miR￣26b￣5p expression (63.79% vs. 25.81% , χ2 /P = 12.550/
0.000); the degree of differentiation was low, TNM stage III￣IV , Lymph node metastasis, high QPRT expression, and low
１２. ５５０ / ０. ０００) ꎻ分化程度低、ＴＮＭ 分期Ⅲ ~ Ⅳ期、淋巴结发生转移、ＱＰＲＴ 高表达、ｍｉＲ￣２６ｂ￣５ｐ 低表达是影响乳腺癌患
者术后复发转移的危险因素[ ＯＲ(９５％ ＣＩ) ＝ ２. ３８１(１. ３９４ ~ ４. ０６６) 、２. ３１７(１. ２７２ ~ ４. ２２１) 、２. ４４９(１. ２４３ ~ ４. ８２５) 、
４７６
疑难病杂志 ２０２１ 年 ５ 月第 ２０ 卷第 ５ 期 Ｃｈｉｎ Ｊ Ｄｉｆｆｉｃ ａｎｄ Ｃｏｍｐｌ ＣａｓꎬＭａｙ ２０２１ꎬＶｏｌ. ２０ Ｎｏ. ５
【 ＤＯＩ】 １０. ３９６９ / ｊ. ｉｓｓｎ. １６７１￣６４５０. ２０２１. ０５. ００９
论著临床
ＱＰＲＴ、ｍｉＲ￣２６ｂ￣５ｐ 在乳腺癌中表达及其
７. ９４９ / ０. ００５) ꎻＱＰＲＴ 高表达患者术后复发转移率明显高于 ＱＰＲＴ 低表达患者(６５. ３８％ ｖｓ. ２９. ７３％ ꎬχ２ / Ｐ ＝ １３. ２６０ /
０. ０００) ꎬｍｉＲ￣２６ｂ￣５ｐ 低表达 患 者 术 后 复 发 转 移 率 明 显 高 于 ｍｉＲ￣２６ｂ￣５ｐ 高 表 达 患 者 ( ６３. ７９％ ｖｓ. ２５. ８１％ ꎬ χ２ / Ｐ ＝
Ｃｏｒｒｅｓｐｏｎｄｉｎｇ ａｕｔｈｏｒ:Ｈｕ Ｙｕｐｉｎｇꎬ Ｅ￣ｍａｉｌ: Ｈｎｘｙ ｋｊｙｈ＿ｂｘ＠ １６３. ｃｏｍ
Ｆｕｎｄｉｎｇ ｐｒｏｇｒａｍ: Ｇｕａｎｇｄｏｎｇ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ ａｎｄ Ｔｅｃｈｎｏｌｏｇｙ Ｒｅｓｅａｒｃｈ Ｆｕｎｄ Ｐｒｏｊｅｃｔ ( Ａ２０１８０７４) ꎻ Ｇｕａｎｇｚｈｏｕ Ｈｕａｄｕ Ｄｉｓｔｒｉｃｔ
组) ꎬ免疫组化法检测 ＱＰＲＴ 表达ꎬ原位杂交技术检测 ｍｉＲ￣２６ｂ￣５ｐ 表达水平ꎬ分析 ＱＰＲＴ、ｍｉＲ￣２６ｂ￣５ｐ 水平与患者术后
复发转移的关系ꎬＣＯＸ 多因素分析乳腺癌患者发生复发转移的影响因素ꎮ 结果 观察组 ＱＰＲＴ 的阳性表达率明显高
于对照组ꎬｍｉＲ￣２６ｂ￣５ｐ 的阳性表达率明显低于对照组( χ２ / Ｐ ＝ ５４. ３９５ / ０. ０００、２３. ４４８ / ０. ０００) ꎻＱＰＲＴ 与 ｍｉＲ￣２６ｂ￣５ｐ 在
【 Ｋｅｙ ｗｏｒｄｓ】 Breast cancer; Quinolinic acid phosphoribosyl transferase; microRNA￣26b￣5p; Prognosis; Correlation
Ｓｃｉｅｎｃｅ ａｎｄ Ｔｅｃｈｎｏｌｏｇｙ Ｐｒｏｊｅｃｔ (１８￣ＨＤＷＳ￣０２３)
【 Ａｂｓｔｒａｃｔ】 Ｏｂｊｅｃｔｉｖｅ To analyze the expression of quinolinic acid phosphoribosyl transferase (QPRT) and mi￣
croRNA￣26b￣5p (miR￣26b￣5p) in breast cancer and their relationship with the prognosis of patients.Ｍｅｔｈｏｄｓ From March
2015 to October 2017, 89 female patients undergoing modified radical mastectomy in the Breast Department of Guangzhou
miR￣26b￣5p expression are risk factors affecting postoperative recurrence and metastasis of breast cancer patients [ OR
(95% CI) = 2.381 (1.394 － 4.066), 2.317 (1.272 － 4.221 ), 2.449 (1.243 － 4.825 ), 2.604 (1.501 － 4.517 ), 3.007 (1.6ｓｉｏｎ The up￣regulation of QPRT expression in breast cancer tissues and the down￣regulation of miR￣26b￣
5p expression are risk factors affecting postoperative recurrence and metastasis of breast cancer patients, and may provide
２. ６０４(１. ５０１ ~ ４. ５１７) 、３. ００７(１. ６２５ ~ ５. ５６４) ] ꎮ 结论 乳腺癌组织中 ＱＰＲＴ 表达上调ꎬｍｉＲ￣２６ｂ￣５ｐ 表达下调ꎬ是影响
乳腺癌患者术后发生复发转移的危险因素ꎬ可能为临床治疗乳腺癌提供潜在靶点ꎮ
【 关键词】 乳腺癌ꎻ喹啉酸磷酸核糖基转移酶ꎻ微小 ＲＮＡ￣２６ｂ￣５ｐꎻ预后ꎻ相关性
level and postoperative recurrence and metastasis was analyzed. Factor analysis of the influencing factors of recurrence and
metastasis of breast cancer patients.Ｒｅｓｕｌｔｓ The positive expression rate of QPRT in the observation group was significant￣
【 中图分类号】 Ｒ７３７. ９ 【 文献标识码】 Ａ
Ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＱＰＲＴ ａｎｄ ｍｉＲ￣２６ｂ￣５ｐ ｉｎ ｂｒｅａｓｔ ｃａｎｃｅｒ ａｎｄ ｔｈｅｉｒ ｒｅｌａｔｉｏｎｓｈｉｐ ｗｉｔｈ ｔｈｅ ｐｒｏｇｎｏｓｉｓ ｏｆ ｐａｔｉｅｎｔｓ Ｙｕａｎ
【 摘 要】 目的 分析喹啉酸磷酸核糖基转移酶( ＱＰＲＴ) 、微小 ＲＮＡ￣２６ｂ￣５ｐ( ｍｉＲ￣２６ｂ￣５ｐ) 在乳腺癌中表达及其
与患者预后的关系ꎮ 方法 选取 ２０１５ 年 ３ 月—２０１７ 年 １０ 月于广州市中西医结合医院行乳腺癌( 经病理组织检查均
为乳腺浸润性导管癌) 改良根治术女性患者 ８９ 例为研究对象ꎬ获取乳腺癌组织( 观察组) 和癌旁正常乳腺组织( 对照
pression was negatively correlated (r/P = － 0.343/0.000 ). The expression levels of QPRT and miR￣26b￣5p were related to
疑难病杂志 ２０２１ 年 ５ 月第 ２０ 卷第 ５ 期 Ｃｈｉｎ Ｊ Ｄｉｆｆｉｃ ａｎｄ Ｃｏｍｐｌ ＣａｓꎬＭａｙ ２０２１ꎬＶｏｌ. ２０ Ｎｏ. ５
Hospital of Integrated Traditional Chinese and Western Medicine (all were invasive ductal carcinoma of the breast after path￣
ological examination) were selected as the research objects, and breast cancer tissues were obtained. (Observation group)
and normal breast tissue adjacent to cancer (control group), immunohistochemical method was used to detect QPRT expres￣
sion, in situ hybridization was used to detect miR￣26b￣5p expression level, and the relationship between QPRT, miR￣26b￣5p
tients with high QPRT expression was significantly higher than that of patients with low QPRT expression (65.38% vs.
29.73% , χ2 /P = 13.260/0.000 ), the postoperative recurrence and metastasis rate of patients with low miR￣26b￣5p expres￣
ly higher than that of the control group, and the positive expression rate of miR￣26b￣5p was significantly lower than that of
the control group (χ2 /P = 54.395/0.000, 23.448/0.000); QPRT and miR￣26b￣5p were found in breast cancer tissues The ex￣
乳腺癌组织中表达呈负相关( ｒ / Ｐ ＝ － ０. ３４３ / ０. ０００) ꎻＱＰＲＴ、ｍｉＲ￣２６ｂ￣５ｐ 表达水平与患者 ＴＮＭ 分期、肿瘤分化程度、淋
巴结转移有关( ＱＰＲＴ:χ２ / Ｐ ＝ ５. ３２６ / ０. ０２１、４. ９９１ / ０. ０２５、６. ７４０ / ０. ００９ꎬｍｉＲ￣２６ｂ￣５ｐ:χ２ / Ｐ ＝ ５. ９０２ / ０. ０１５、４. ９４６ / ０. ０２６、
Ｎａｎｇｕｉ ∗ ꎬ Ｌｕｏ Ｘｕｅｐｉｎｇꎬ Ｈｕ Ｙｕｐｉｎｇꎬ Ｙａｏ Ｂｉｎꎬ Ｈｕａｎｇ Ｗｅｉｌｉｎｇ.
∗
Ｄｅｐａｒｔｍｅｎｔ ｏｆ Ｍａｍｍａｒｙ Ｇｌａｎｄꎬ Ｇｕａｎｇｚｈｏｕ Ｉｎｔｅｇｒａｔｅｄ Ｔｒａ￣
ｄｉｔｉｏｎａｌ Ｃｈｉｎｅｓｅ ａｎｄ Ｗｅｓｔｅｒｎ Ｍｅｄｉｃｉｎｅ Ｈｏｓｐｉｔａｌꎬ Ｇｕａｎｇｄｏｎｇ ＰｒｏｖｉｎｃｅꎬＧｕａｎｇｚｈｏｕ ５１０８００ꎬＣｈｉｎａ
与患者预后的关系
袁南贵ꎬ罗雪平ꎬ胡玉萍ꎬ姚斌ꎬ黄伟玲
基金项目: 广东省医学科学技术研究基金项目( Ａ２０１８０７４) ꎻ广州市花都区科技计划项目(１８￣ＨＤＷＳ￣０２３)
作者单位: ５１０８００ 广州市中西医结合医院乳腺科( 袁南贵、胡玉萍、姚斌、黄伟玲) ꎬ检验科( 罗雪平)
通信作者: 胡玉萍ꎬＥ￣ｍａｉｌ:Ｈｎｘｙｋｊｙｈ￣ｂｘ＠ １６３. ｃｏｍ
４７７
the patient’ s TNM stage, tumor differentiation, and lymph node metastasis (QPRT: χ2 /P = 5.326/0.021, 4.991/0.025, 6.740/
0.009, miR￣26b￣5p: χ2 /P = 5.902/0.015, 4.946/0.026, 7.949/0.005 ); the postoperative recurrence and metastasis rate of pa￣
sion was significantly higher than that of patients with high miR￣26b￣5p expression (63.79% vs. 25.81% , χ2 /P = 12.550/
0.000); the degree of differentiation was low, TNM stage III￣IV , Lymph node metastasis, high QPRT expression, and low
１２. ５５０ / ０. ０００) ꎻ分化程度低、ＴＮＭ 分期Ⅲ ~ Ⅳ期、淋巴结发生转移、ＱＰＲＴ 高表达、ｍｉＲ￣２６ｂ￣５ｐ 低表达是影响乳腺癌患
者术后复发转移的危险因素[ ＯＲ(９５％ ＣＩ) ＝ ２. ３８１(１. ３９４ ~ ４. ０６６) 、２. ３１７(１. ２７２ ~ ４. ２２１) 、２. ４４９(１. ２４３ ~ ４. ８２５) 、
４７６
疑难病杂志 ２０２１ 年 ５ 月第 ２０ 卷第 ５ 期 Ｃｈｉｎ Ｊ Ｄｉｆｆｉｃ ａｎｄ Ｃｏｍｐｌ ＣａｓꎬＭａｙ ２０２１ꎬＶｏｌ. ２０ Ｎｏ. ５
【 ＤＯＩ】 １０. ３９６９ / ｊ. ｉｓｓｎ. １６７１￣６４５０. ２０２１. ０５. ００９
论著临床
ＱＰＲＴ、ｍｉＲ￣２６ｂ￣５ｐ 在乳腺癌中表达及其
７. ９４９ / ０. ００５) ꎻＱＰＲＴ 高表达患者术后复发转移率明显高于 ＱＰＲＴ 低表达患者(６５. ３８％ ｖｓ. ２９. ７３％ ꎬχ２ / Ｐ ＝ １３. ２６０ /
０. ０００) ꎬｍｉＲ￣２６ｂ￣５ｐ 低表达 患 者 术 后 复 发 转 移 率 明 显 高 于 ｍｉＲ￣２６ｂ￣５ｐ 高 表 达 患 者 ( ６３. ７９％ ｖｓ. ２５. ８１％ ꎬ χ２ / Ｐ ＝
Ｃｏｒｒｅｓｐｏｎｄｉｎｇ ａｕｔｈｏｒ:Ｈｕ Ｙｕｐｉｎｇꎬ Ｅ￣ｍａｉｌ: Ｈｎｘｙ ｋｊｙｈ＿ｂｘ＠ １６３. ｃｏｍ
Ｆｕｎｄｉｎｇ ｐｒｏｇｒａｍ: Ｇｕａｎｇｄｏｎｇ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ ａｎｄ Ｔｅｃｈｎｏｌｏｇｙ Ｒｅｓｅａｒｃｈ Ｆｕｎｄ Ｐｒｏｊｅｃｔ ( Ａ２０１８０７４) ꎻ Ｇｕａｎｇｚｈｏｕ Ｈｕａｄｕ Ｄｉｓｔｒｉｃｔ
组) ꎬ免疫组化法检测 ＱＰＲＴ 表达ꎬ原位杂交技术检测 ｍｉＲ￣２６ｂ￣５ｐ 表达水平ꎬ分析 ＱＰＲＴ、ｍｉＲ￣２６ｂ￣５ｐ 水平与患者术后
复发转移的关系ꎬＣＯＸ 多因素分析乳腺癌患者发生复发转移的影响因素ꎮ 结果 观察组 ＱＰＲＴ 的阳性表达率明显高
于对照组ꎬｍｉＲ￣２６ｂ￣５ｐ 的阳性表达率明显低于对照组( χ２ / Ｐ ＝ ５４. ３９５ / ０. ０００、２３. ４４８ / ０. ０００) ꎻＱＰＲＴ 与 ｍｉＲ￣２６ｂ￣５ｐ 在