β-榄香烯注射液对人肝癌HepG-2细胞微管蛋白β的影响(英文)

β-榄香烯注射液对人肝癌HepG-2细胞微管蛋白β的影响
(英文)
Yuqiu Mao;Liying Ban;Jielin Zhang;Li Hou;Xiaonan Cui
【期刊名称】《中德临床肿瘤学杂志：英文版》
【年(卷),期】2014()2
【摘要】Objective: The aim of this study was to investigate the impact of beta-elemene injection on the growth and beta-tubulin of human hepatocarcinoma HepG2 cells. Methods: Cell proliferation was assessed by MTT assay. Cell cycle distribution was detected by flow cytometry(FCM). The mRNA expression of beta-tubulin was measured by RT-PCR. Western blot analysis was used to determine protein expression of beta-tubulin and the polymerization of beta-tubulin. Results: Beta-elemene injection inhibited HepG2 cells proliferation in a dose- and time-dependent manner; FCM analysis indicated beta-elemene injection induced cell cycle arrested at S phase. RT-PCR and western-blot analysis showed that beta-elemene injection down-regulated beta-tubulin expression at both mRNA and protein levels, presenting a dose-dependent manner. Moreover, beta-elemene injection reduced the polymerization of microtubules in a dose-dependent manner. Conclusion: Beta-elemene injection can inhibit the proliferation of hepatoma HepG2 cells, the mechanism might be partly related to the down-regulation of beta-tubulin and inhibition of microtubular polymerization.
【总页数】6页(P69-74)
【关键词】HepG2细胞;β-榄香烯;肝癌细胞;RT-PCR检测;β-微管蛋白;时间依赖性;流式细胞仪分析;细胞增殖
【作者】Yuqiu Mao;Liying Ban;Jielin Zhang;Li Hou;Xiaonan Cui
【作者单位】Department of Oncology,The First Affiliated Hospital of Dalian Medical University;Department of Pathology,Dalian Medical University 【正文语种】中文
【中图分类】Q513;TQ231.3
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