运动性心肌缺血损伤模型大鼠的制备

运动性心肌缺血损伤模型大鼠的制备
彭峰林;黄丽丽;郭艳菊
【期刊名称】《中国组织工程研究》
【年(卷),期】2016(020)040
【摘要】背景：运动性心肌缺血动物模型的建立没有统一的标准，导致研究结果
的不可比性，影响了运动医学特别是运动心血管领域研究的推进，所以建立统一的运动性心肌缺血动物模型势在必行。

<br> 目的：探索建立跑台运动性心肌缺
血损伤大鼠模型的方法。

<br> 方法：96只SD雌性大鼠被随机分为安静对照组、异丙肾上腺素药物模型组和10个运动组(中强度力竭运动1，3次组、中强度力竭运动1，2，3周组、高强度力竭运动1，3次组、高强度力竭运动1，2，3
周组)，取各组心肌组织进行苏木精-伊红染色病理分析，检测血清心肌酶指标和肌钙蛋白I水平， RT-PCR检测bcl-2和bax基因表达。

<br> 结果与结论：①心肌苏木精-伊红染色检测结果：随着运动时间的延长损伤程度越来越严重，且高
强度运动组比中强度运动组损伤程度更严重；②血清心肌酶指标和肌钙蛋白I水平：中强度力竭运动自1周开始，血清谷草转氨酶与乳酸脱氢酶活性出现显著性升高(P<0.05或P<0.01)，高强度力竭性运动训练3次开始，谷草转氨酶与乳酸脱氢酶活性显著性升高(P<0.05或P<0.01)。

各组肌钙蛋白I含量的变化趋势与谷草转氨酶、乳酸脱氢酶活性变化基本相同，但中强度力竭运动需2周肌钙蛋白I含量才比安静对照组显著升高；③细胞凋亡调控基因表达：各运动组bcl-2/bax比值均显
著性低于安静对照组(P<0.05或P<0.01)，高强度1次组(P<0.01)和3次组
(P<0.05)明显高于异丙肾上腺素药物模型组，中强度各组均高于异丙肾上腺素药
物模型组(P<0.05或P<0.01)；④结果表明，中强度跑台力竭运动3周开始、高强
度跑台力竭运动2周开始的运动模型都可作为运动性心肌缺血损伤模型，病理分析、心肌血清酶谱、肌钙蛋白I可作为建立运动性心肌缺血损伤模型的评估指标，细胞凋亡调控基因只可作为参考指标。

%BACKGROUND:No uniform standard for constructing the animal model of exercise-induced myocardial ischemia injury results in the incomparability among research results and impedes the development of sport medicine especial y in the cardiovascular field;thereby, it is imperative to reach an agreement in constructing criteria. <br> OBJECTIVE:To explore the method of establishing the rat model of myocardial ischemia induced by running. <br> METHODS:Total y 96 female Sprague-Dawley rats were randomly divided into rest control group, isoprenaline group and 10 exercise groups (1-and
3-time moderate-intensity exercise groups, 1-, 2-and 3-week moderate-intensity exercise groups, 1-and 3-time high-intensity exercise groups, 1-,
2-and 3-week high-intensity exercise groups). After exhaustive exercise, myocardium was col ected for morphological observation by hematoxylin-eosin staining, serum levels of myocardial enzymes and cardiac troponin I were detected, and the expressions of Bcl-2 and Bax were detected by
real-time PCR. <br> RESULTS AND CONCLUSION:(1) Hematoxylin-eosin staining showed that the damage degree was more severe with the time of exercise, and the high-intensity exercise groups were more severe than those in the moderate-intensity exercise groups. (2) The activity of serum glutamic-oxaloacetic transaminase and lactic dehydrogenase was significantly increased after 1-week moderate-intensity exhaustive exercise (P<0.05 or P<0.01). From the beginning of the 3-time high-intensity
exhaustive exercise, the activity of glutamic-oxaloacetic transaminase and lactic dehydrogenase was significantly increased (P<0.05 or P<0.01). (3) Cardiac troponin I content change trend was basical y the same as glutamic-oxaloacetic transaminase and lactic dehydrogenase changes, but cardiac troponin I content in the moderate-intensity exhaustive exercise groups was significantly higher than that in the rest control group until 2 weeks. The Bcl-2/Bax ratios in al exercise groups were significantly lower than that in the rest control group (P<0.05 or P<0.01);those in the 1-and 3-time high-intensity exercise groups were significantly higher than in the isoprenaline group (P<0.05 or P<0.01);and those in moderate-intensity groups were higher than in the isoprenaline group (P<0.05 or P<0.01). (4) In conclusion, 2-week high-intensity and 3-week moderate-intensity exhaustive exercise can induce myocardial ischemia injury, and pathological analysis, serum levels of myocardial enzymes and cardiac troponin I can be used as the evaluation indexes, while apoptosis regulation genes just as the reference index.
【总页数】7页(P6007-6013)
【作者】彭峰林;黄丽丽;郭艳菊
【作者单位】广西师范大学体育学院，广西壮族自治区桂林市 541004;开平市忠源纪念中学，广东省开平市 529300;广西师范大学体育学院，广西壮族自治区桂林市 541004
【正文语种】中文
【中图分类】R318
【相关文献】
1.实验性大鼠脊髓损伤模型的制备及研究进展 [J], 李晓彬;刘涛;周俊锋;范磊
2.大鼠心肌缺血再灌注损伤模型的制备及改进 [J], 陈靖宜;刘菊英
3.利用定量加压充气球囊制备大鼠心肌缺血再灌注损伤模型 [J], 许士进;何淑芳;金世云;胡军;张野
4.运动性心肌缺血损伤模型大鼠的制备 [J], 彭峰林;黄丽丽;郭艳菊;
5.胸骨下段切口制备大鼠心肌缺血/再灌注损伤模型的评价 [J], 刘勇;殷桂林;朱水波;孙宗全
因版权原因，仅展示原文概要，查看原文内容请购买。