北五味子配方颗粒提取工艺的正交设计试验优选

北五味子配方颗粒提取工艺的正交设计试验优选
叶绿萍;李启军;刘小意
【期刊名称】《中国医药导报》
【年(卷),期】2011(8)25
【摘要】Objective: To optimize the optimal preparation procedure for Schisandrin chinensis Baill.Granules.Methods: The influential factors of water comsumption, extraction times and extraction time were observed by orthogonal design with the recovery rate, deoxyschisandrin and γ-schisandrin content in schisandrin chinensis baill.which was determined by orthogonal design, then chose the optimized extraction technology.Results: The line ar relation of DSSD and γ-SSD were good in the range from 0.265-1.590 μg and 0.638-3.188 μg (r=l.000 0, r=0.999 9).The average recovery was 101.12％ and 100.38％, RSD was 1.75％ and 1.10％ respectively.The main factors of affecting Schisandrin chinensis Baill.recovery rate, DSSD, γ-SSD were extraction times, extraction time, water consumption successively.The optimal recovery process was found to be three single reflux with 10 times of water for 3 times, and every time 2.0 h.The average content of DSSD was 0.024％and γ-SSD was 0.049％, the average extraction rate was 36.12％.Conclusion: The optimal preparation procedure is stable, and it can be the reference to the extraction technology of Schisandrin chinensis Baill..%目的:优选北五味子配方颗粒的最佳提取工艺.方法:采用正交设计安排试验,以提取率、五味子甲素(DSSD)、五味子乙素(γ-SSD)的含
量作为考察指标,考察用水量、提取时间、提取次数对五味子配方颗粒提取工艺条件的影响,优选出合理的提取工艺.结果:在既定的HPLC测定方法下,北五味子配方颗粒中DSSD及γ-SSD含量分别在0.265～1.590 μg和0.638～3.188 μg范围内,进样量与峰面积的线性关系良好(r=1.000 0和r=0.999 9),平均回收率分别为101.12%和100.38%,RSD值分别为1.75%和1.10%.影响北五味子配方颗粒提取率、DSSD、γ-SSD含量的主要因素依次为提取次数、提取时间、用水量,最佳工艺条件为:加水量为10倍,提取3次,每次2 h,测得DSSD平均含量为0.024%,γ-SSD平均含量为0.049%,平均提取率为36.12%.结论:该提取工艺具有提取率稳定性好、五味子甲素及五味子乙素得率高、操作简单且成本较低的特点,可为五味子配方颗粒的提取工艺提供参考.
【总页数】3页(P62-63,68)
【作者】叶绿萍;李启军;刘小意
【作者单位】广西中医学院一附院药学部,广西南宁,530023;广西壮族自治区南宁市武鸣县人民医院药剂科,广西南宁,530100;广西中医学院一附院药学部,广西南宁,530023
【正文语种】中文
【中图分类】R284.2
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