A Bio-inspired Interpolation Kernel for Medical Image Processing Implemented on DSP Processor
PPARγ基因shRNA真核表达载体的构建及其干扰效果鉴定
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P P A M基因shRNA真核表达载体的构建及其干扰效果鉴定辛婧、沈亚非、邓飞\谢亚争、张日华2,刘云21.漯河市中心医院内分泌科,河南漯河462000;2•南京医科大学第一附属医院老年医学内分泌科摘要:目的构建过氧化物酶体增殖物激活受体y(PPARy)基因RNA干扰的真核表达载体,转染小鼠3T3-L1前体脂肪 细胞,并鉴定其干扰效果。
方法选择设计2条针对小鼠PPA R y基因的干扰耙序列,构建真核表达载体PLKO. 1-PPARy-GFP-shRNAl/2,以PCR鉴定并进行序列分析。
证实质粒构建成功后,转染小鼠3T3-U前体脂肪细胞,荧光显微镜下观察 绿色荧光蛋白(eGFP)的表达,计算转染效率;采用实时定量PC R法和W estern印迹检测载体对PPA R y基因的表达干扰效 果;采用油红染色观察PPA R y在脂肪细胞分化过程中对脂滴形成的作用。
结果成功构建PPA R y干扰质粒PLKO.1- PPARy-GFP-shRNAl/2,PC R和DNA测序证实序列与设计完全一致;荧光显微镜下观察到3T3-L1细胞绿色荧光蛋白的表 达,证实重组质粒成功转人细胞,转染效率(92. 67士1.53)%;实时荧光定量PC R和Western B lot印记试验均显示PLKO. 1-PPARy-GFP-shRNAl/2转染的3T3-L1细胞PPARy基因分别被特异性抑制;耙点1干扰效率[(78. 2士2.1)%]高于耙点2 [(55.8±4.3)%],差异有统计学意义(P C0.05),靶点1初步鉴定为有效靶点。
油红染色显示PLKO. 1-PPARy-GFP-shR-NA1/2转染均可抑制3T3-L1脂肪细胞分化和脂滴聚集。
结论成功构建了耙向干扰PPA R y基因的shRNA真核表达质粒 PLKO.l-PPARy-GFP-shRNAl/2,并筛选出有效抑制靶基因的表达质粒,初步验证PPA R y基因对脂肪细胞分化的作用,为 进一步研究提供了有效的分子生物学工具。
香港科大发现新起始蛋白质
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香港科大发现新起始蛋白质
阿雅
【期刊名称】《食品信息与技术》
【年(卷),期】2004(000)012
【摘要】香港科技大学生物化学系科研小组最近发现一种新的起始蛋白,该蛋白在DNA(脱氧核糖核酸)复制的起始阶段起关键作用。
专家认为,这一发现开拓了在正常细胞和癌细胞中研究DNA复制的新领域。
据悉,认识DNA复制过程及其调控的机理,对于了解癌症的发展至关重要。
因为,每一个癌细胞在分裂前,必须先行复制其DNA,
【总页数】1页(P61)
【作者】阿雅
【作者单位】无
【正文语种】中文
【中图分类】Q753
【相关文献】
1.香港科大发现新起始蛋白质 [J],
2.华中科大发现新"疼痛基因"揭示疼痛疾病致病原因 [J],
3.中科大发现新铁基超导材料 [J], ;
4.中国科大发现肿瘤精准干预新切入点 [J],
5.中国药科大学发现肝癌治疗新靶标 [J],
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外胚层发育不良受体EDA2R的研究进展
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肿瘤坏死因子受体超级家族(tumor necrosis fac⁃tor receptor superfamily,TNFRSF)的死亡受体(death receptor)以及它们的配体在胚胎正常发育及机体免疫和炎症反应过程中扮演了重要角色。
外胚层发育不良受体(ectodysplasin A2receptor,EDA2R)是一个在20年前被鉴定出来的TNFRSF成员(TNFRSF27)[1],在肿瘤发生、雄激素性脱发等过程中起到重要的作用,但对于该受体作系统性介绍的综述文章尚未见报道。
本文就该受体的研究进展作一系统性的综述,旨在为相关研究提供新的思路。
1EDA2R的蛋白结构和配体1.1EDA2R的蛋白结构EDA2R基因位于人类染色体Xq12,全长约43kb,有6个外显子(GenBank登录号:NG_013271),外胚层发育不良受体EDA2R的研究进展蓝希钳1,2,肖海婷1,2,罗怀容1,2,陈建宁1,2(西南医科大学药学院:1衰老与再生医学实验室,2药理学教研室,四川泸州646000)【摘要】外胚层发育不良受体EDA2R(ectodysplasin A2receptor)是肿瘤坏死因子受体超级家族(tumor necrosis factor recep⁃tor superfamily,TNFRSF)中的一个较新的成员,在发育中的胚胎里有很高的表达,在成年人和动物的多个器官组织中也有表达。
与其它TNFRSF成员不同,尽管EDA2R蛋白在胞内没有死亡结构域(death domain,DD),但它仍可激活NF-κB和JNK通路,并介导细胞的凋亡。
本文广泛回顾了近年来与EDA2R有关的文献,就该蛋白分子的相关研究进展进行综述,以期为与该蛋白相关的分子功能或其介导的相关疾病的研究提供新的思路。
【关键词】EDA2R受体肿瘤坏死因子受体超级家族死亡结构域凋亡【中图分类号】R34文献标志码A doi:10.3969/j.issn.2096-3351.2021.03.018Research progress of ectodysplasin A2receptorLAN Xi-qian1,2,XIAO Hai-ting1,2,LUO Huai-rong1,2,CHEN Jian-ning1,2 1Key Laboratory for Aging and Regenerative Medicine;2Department of Pharmacology,School of Pharmac,South⁃west Medical University,Luzhou646000,Sichuan,China【Abstract】Ectodysplasin A2receptor(EDA2R)is a relatively new member of the tumor necrosis factor re⁃ceptor superfamily(TNFRSF),and it is highly expressed in developing embryos and is also expressed in multiple organs and tissues of adult human and animals.Different from other TNFRSF members,EDA2R protein does not contain the death domain in the intracellular region,but it can still activate the NF-κB and JNK pathways and medi⁃ate cell apoptosis.This article reviews related articles on EDA2R in recent years and related research advances in this protein,in order to provide new ideas for research on molecular functions associated with EDA2R or related dis⁃eases mediated by EDA2R.【Key words】Ectodysplasin A2receptor Tumor necrosis factor receptor superfamily Death domain Apoptosis基金项目:泸州市科技局-西南医科大学联合项目(2018LZXNYD-ZK12);西南医科大学-泸州市中医医院基地项目(2019-LH005)第一作者简介:蓝希钳,博士。
基于网络药理学探讨白藜芦醇治疗肺癌的生物分子机制
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基于网络药理学探讨白藜芦醇治疗肺癌的生物分子机制 张丽慧,耿其顺,朱子家,王文斌,沈志博,李砺锋,薛文华,赵杰郑州大学第一附属医院,河南郑州 450052摘要:目的 基于网络药理学,运用在线数据库研究白藜芦醇治疗肺癌的潜在作用机制。
方法 通过中药系统药理学数据库与分析平台(TCMSP)、PubChem、SwissTargetPrediction、GeneCards数据库分别获取白藜芦醇和肺癌的相关基因,取二者交集基因,使用String数据库获得交集基因的蛋白相互作用(PPI)网络图,使用DA VID6.8对交集基因进行GO和KEGG富集分析。
采用Cytoscape3.7.2软件构建化合物-靶点-通路-疾病网络模型。
使用AutoDock4.2.6软件对白藜芦醇和重要靶点进行分子对接。
Western blot检测白藜芦醇对人肺癌H1975细胞p-Akt蛋白表达的影响。
结果 获得白藜芦醇和肺癌交集基因78个,PPI网络图表明交集基因关系密切。
富集分析得到生物过程55项、分子功能26项、细胞组分18项,以及86条相关通路,其中以PI3K-Akt 通路富集靶点较多。
分子对接结果显示,白藜芦醇与PIK3CB、PIK3CA这2个重要靶点均能稳定结合。
Western blot检测结果显示,白藜芦醇能够显著降低人肺癌H1975细胞p-Akt蛋白表达。
结论 白藜芦醇可能主要通过作用于PIK3CB、PIK3CA靶点,介导PI3K-Akt信号通路发挥治疗肺癌作用。
关键词:白藜芦醇;肺癌;网络药理学;作用机制;分子对接中图分类号:R273.42;R285 文献标识码:A 文章编号:1005-5304(2021)06-0046-06DOI:10.19879/ki.1005-5304.202004113 开放科学(资源服务)标识码(OSID):Biomolecular Mechanism of Resveratrol Against Lung Cancer Based on Network Pharmacology ZHANG Lihui, GENG Qishun, ZHU Zijia, WANG Wenbin, SHEN Zhibo, LI Lifeng, XUE Wenhua, ZHAO Jie First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China Abstract:Objective To use the online databases to study the potential mechanism of resveratrol in treating lung cancer based on network pharmacology. Methods The genes related to resveratrol and lung cancer were obtained from TCMSP, PubChem, SwissTargetPrediction and GeneCards database, and the related intersection genes of resveratrol and lung cancer were screened out. The String database was used to draw the PPI network diagram of the intersection genes, and the DA VID 6.8 was used to perform GO and KEGG enrichment analysis. The compound-target-signal pathway-disease network model was constructed by using Cytoscape 3.7.2 software. AutoDock 4.2.6 software was used to perform molecular docking of resveratrol and important targets. Western blot was used to detect the effect of resveratrol on the expression of p-Akt protein in human lung cancer H1975 cell line. Results Totally 78 intersection genes of resveratrol and lung cancer were obtained, and the PPI network diagram showed that there was a close relationship between them. The enrichment analysis mainly obtained 55 biological processes, 26 molecular functions, 18 cellular components, and 86 related pathways, among which the PI3K-Akt pathway enriched the most targets. Molecular docking results showed that resveratrol could stably bind to two important targets, PIK3CB and PIK3CA. Western blot test results showed that resveratrol could significantly reduce the expression of p-Akt protein in human lung cancer H1975 cells. Conclusion Resveratrol may mainly act on the targets of PIK3CB and PIK3CA, and mediates the PI3K-Akt signaling pathway to exert anti-lung cancer action.Keywords: resveratrol; lung cancer; network pharmacology; action mechanism; molecular docking研究表明,肿瘤的发生发展与基因突变及多条信号通路改变有关[1-2]。
一种从大熊猫粪便中提取DNA的改进方法
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动物学报49 (5) :670~674 , 2003Acta Zoologica S i nica一种从大熊猫粪便中提取D NA 的改进方法3钟华①赖旭龙②魏荣平③刘中来①33( ①华中师范大学生命科学学院, 武汉430079)( ②中国地质大学地球科学学院, 武汉430074) ( ③中国保护大熊猫研究中心, 四川卧龙623006)摘要本研究描述一个改进的方法, 使从大熊猫粪便中提取DNA 用于PCR 扩增变得更加容易。
在粪便DNA 的提取过程中采用一个新的预处理方法, 将粪便用预冷的丙酮洗2~3 次, 除去粪便中含有的大量PCR 抑制物, 然后用蛋白酶K 裂解、酚- 氯仿抽提, 能提取到纯度很高的DNA 供PCR 扩增。
本实验PCR 扩增了大熊猫脑源性神经营养因子(BDNF) 基因和线粒体细胞色素 b 基因片段, 并进行测序分析, 证实了提取的可靠性。
对比本方法和未经丙酮预处理的方法提取的DNA 进行PCR 扩增, 前者的扩增结果明显优于后者[ 动物学报49 (5) : 670~674 , 2003 ] 。
关键词大熊猫粪便DNA 丙酮DNA 抽提非损伤性取样An improved protocol for D NA extraction from the faeces of the giant panda 3 ZHON G Hua ① LA I Xu2Long ② W EI Rong2Ping ③ L IU Zhong2Lai ①33( ①College of L if e S cience , Cent ral China Nor mal U niversit y , W uh an430079 , China)( ②Faculty of Earth Sciences , China U niversit y of Geosciences , W uhan430074 , China)( ③China Cons ervation and Res earch Center f or th e Gi ant Pan da , W olong623006 , S ichuan , China) Abstract An improved method that facilitates the extraction of PCR2compatible faecal DNA from giant pand a’s faeces is described. The method involved a novel preprocessing step in DNA extraction. The faeces was washed two or three timeswith precooled acetone , which removed numerous potential PCR inhibitors , and then digested with proteinase K. The DNA was purified with phenol/ chloroform. The faecal DNA obtained was sufficiently pure to support reliable amplifica2 tion , and was applied as template DNA to amplify a portion of the giant panda brain derived neurotrophic factor (BDNF) gene and mitochondrial cytochrome b gene. The sequenced results of PCR products confirmed that the extracted DNA was from the giant panda. Comparison with the PCR products demonstrated that the faecal DNA extracted b y the improved protocol was better than the faecal DNA extracted without acetone preprocessing. [ Acta Zoologica S inica 49 (5) : 670 - 674 , 2003 ] .K ey words Giant panda ( A il uropoda melanoleuca) , Faecal DNA , Acetone , DNA extraction , Noninvasive sampling在大熊猫的遗传多样性、种群数量调查、进化和分类、亲子鉴定等研究中, DNA 分析是重要的研究手段。
源于人诱导多能干细胞并表达脑啡肽酶2的巨噬细胞可降解β淀粉样蛋白
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源于人诱导多能干细胞并表达脑啡肽酶2的巨噬细胞可降解
β淀粉样蛋白
黄翠芹
【期刊名称】《中国病理生理杂志》
【年(卷),期】2015(000)004
【摘要】近日,日本科学家发现人诱导多能干细胞(iP S细胞)衍生的巨噬细胞在阿尔茨海默病(AD)的治疗中具有应用前景。
在前期研究中,他们建立了从人iP S 细胞生成具有增殖活性的巨噬细胞样髓系细胞(iP S-ML)的技术,并发现iP S-ML 能降低加入到培养基中的Aβ水平,且iP S-ML的培养上清液能减轻Aβ的神经毒性。
在该研究中,他们又构建了表达Aβ特异性单链抗体Fc受体融合形式(anti-AβscF v)的iP S-ML;此外,还让iP S-ML表达脑啡肽酶2(NEP2;一种能够降解Aβ的蛋白酶)。
【总页数】1页(P614-614)
【作者】黄翠芹
【作者单位】
【正文语种】中文
【相关文献】
1.人参皂苷Rg1对脂多糖诱导的C6细胞株淀粉样前体蛋白和脑啡肽酶表达的影响
2.SCD-1在β淀粉样蛋白1-40诱导的巨噬细胞中表达的初步研究
3.人参皂苷Rg1对脂多糖抑制的U251细胞株脑啡肽酶表达的影响
4.人参皂苷Rg_1对LPS
诱导的SK-N-SH细胞株脑啡肽酶表达的影响5.血清淀粉样蛋白A对THP-1巨噬细胞炎症反应及SR—BI表达的影响
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免疫共沉淀联合质谱分析筛选克罗恩病中Intelectin-1相互作用蛋白的初步研究

免疫共沉淀联合质谱分析筛选克罗恩病中Intelectin-1相互作用蛋白的初步研究顾问;周郑;吴文涌;刘晓昌;余昌俊【摘要】Objective To screen the proteins interacted with Intelectin-1 in Crohn disease via comparing Crohn disease and normal tissues and investigate the function of Intelectin-1 in the development of Crohn disease.Methods The proteins interacting with Intelectin-1 were screened by co-immunoprecipitation and identified by MALDL-TOF/TOF-MS and finally confirmed by immunoprecipitation and Western blotanalysis.Results Data of Western blot showed that Intelectin-1 was overexpressed in Crohn disease tissues compared with normal tissues. In addition, four novel proteins(ATPase,HSP90,TRAF3 and ZNF) interacted with Intelectin-1 were successfully verified by MALDL-TOF/TOF-MS. Conclusion Intelectin-1 involves in the development of Crohn disease via interacting with TRAF3, ATPase, HSP90 and ZNF.%目的对比克罗恩病患者的病变与正常肠黏膜,筛选与肠凝集素-1(Intelectin-1)相互作用的差异蛋白,探讨Intelectin-1及其相互作用蛋白在克罗恩病发展中的作用.方法利用免疫共沉淀技术筛选克罗恩病患者病变及正常组织中与Intelectin-1相互作用的蛋白;利用MALDI-TOF/TOF-MS质谱分析技术鉴定与Intelectin-1相互作用蛋白;并使用免疫共沉淀和Western blot分析技术验证所鉴定的Intelectin-1相互作用蛋白.结果利用免疫共沉淀联合质谱分析技术成功筛选出4个与Intelectin-1相互作用差异蛋白,包括:肿瘤坏死因子受体相关因子3(TRAF3)、腺苷酸三磷酸酶(ATPase)、热休克蛋白90(HSP90)、锌指蛋白(ZNF).结论 Intelectin-1可能通过与TRAF3、ATPase、HSP90、ZNF蛋白相互作用,而影响克罗恩病的发展.【期刊名称】《安徽医科大学学报》【年(卷),期】2017(052)004【总页数】5页(P519-523)【关键词】克罗恩病;Intelectin-1;免疫共沉淀;质谱;蛋白质相互作用【作者】顾问;周郑;吴文涌;刘晓昌;余昌俊【作者单位】安徽医科大学第一附属医院普外科,合肥 230022;安徽医科大学第一附属医院普外科,合肥 230022;安徽医科大学第一附属医院普外科,合肥 230022;安徽医科大学第一附属医院消化内科,合肥 230022;安徽医科大学第一附属医院普外科,合肥 230022【正文语种】中文【中图分类】R574.6肠凝集素-1 ( Intelectin-1 )是一种主要表达于人小肠、结肠等组织的分泌性糖蛋白,含313个氨基酸,由1个分泌序列和1个纤维蛋白原相关结构域组成的分子量为120 ku的同源三聚体[1]。
大分子拥挤体系中SPI-葡聚糖共价复合物制备
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P4HA2通过激活PI3KAKTmTOR信号通路促进肝癌的发生和发展
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肝细胞癌(HCC )近几十年来发病率上升,虽然在临床和实验性癌症治疗方面取得了很大进展,但由于术后肿瘤复发和转移率高,HCC 患者的总体预后较差[1-3]。
肝癌的发生发展可能是一个多因素、多步骤的过程[3],但目前关于其具体的分子机制尚不清楚。
因此,更好地了解HCC 发生发展的分子机制对肝癌靶向治疗具有重要意义。
细胞外基质(ECM )由多种大分子组成,包括胶原蛋白、纤维连接蛋白、弹性蛋白、层粘连蛋白、透明质酸和蛋白多糖[4]。
ECM 作为肿瘤微环境中含量最丰富的成分,可以调控肿瘤细胞行为和肿瘤进展,胶原蛋白是ECM 的主要成分,具有促进肿瘤发展的作用,例如IP4HA2promotes occurrence and progression of liver cancer by regulating the PI3K/Akt/mTOR signaling pathwaySHANG Ling 1,JIANG Wendi 1,ZHANG Junli 1,WU Wenjuan 1,21Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis,2Department of Biochemistry and Molecular Biology,School of Laboratory Medicine,Bengbu Medical College,Bengbu 233030,China摘要:目的探讨脯氨酸4-羟化酶II (P4HA2)在肝癌细胞发生发展中的作用及相关机制。
方法利用GEPIA 、Human Protein Atlas 数据库预测P4HA2在肝癌中的表达情况,利用K-M plotter 在线数据库分析P4HA2的表达情况与肝癌预后的关系,采用qRT-PCR 和Western blot 检测肝癌细胞和正常肝细胞中P4HA2的表达。
【高中生物】Nature:饿死肿瘤的新方法
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【高中生物】Nature:饿死肿瘤的新方法RPF的9,12和15位点分别对应核糖体的E,P和A位点肿瘤的生长和代谢可能会局限于某些氨基酸用于蛋白合成。
最近已经被证明,某些类型的癌细胞依赖于甘氨酸、谷氨酰胺,亮氨酸,丝氨酸代谢和增殖。
此外,运用左旋门冬酰胺酶诱导天冬酰胺缺乏,已经用于治疗急性淋巴细胞白血病。
然而,本研究之前还不能在每个肿瘤中检测哪些氨基酸缺乏可以导致其生长受阻。
在2月25日的Nature中,研究者用核糖体分析来检测限制性氨基酸。
他们开发了diricore,一种核糖体检测不同密码子的步骤,用来评估特定氨基酸用于蛋白合成的可用性。
Diricore是基于核糖体分析检测(ribosomeprofiling)而开发的,核糖体分析是一种基于深度测序的技术可以定量地分析核苷酸翻译的方法。
使用核酸酶消化mRNA时,在翻译过程中发挥作用的核糖体结合并保护了大约30bp的mRNA片段(RPF)。
将细胞中这些被保护的mRNA片段构建成DNA文库,再使用测序仪对文库中所有的片段进行深度测序,最终得到了有关细胞中蛋白质翻译情况的图谱。
而在此基础上如图,diricore运用RPF进行亚克隆测序和5’末端密度两种互补的方法进行分析。
他们首先运用代谢抑制和营养缺乏分析验证了diricore的功能与抑制。
值得注意的是使用左旋门冬酰胺酶在天冬酰胺密码子诱导特殊diricore信号会引起高水平天冬酰胺合成酶(ASNS)。
然后研究者将diricore运用到肾癌上,从信号上发现脯氨酸是限制性的氨基酸。
对于天冬酰胺来说,观察的结果与高水平的PYCR1,脯氨酸产生的一个关键酶相关。
这提示了一个允许肿瘤扩增的代偿性的机制。
PYCR1是由脯氨酸前体不足引起的,当脯氨酸缺乏时抑制该酶可以抑制肾癌细胞的增殖。
高水平的PYCR1经常在浸润性的乳腺癌中被观察到。
在这个肿瘤的体内模型系统中,研究人员也发现了脯氨酸是限制性氨基酸。
在限制生长的条件下,需要PYRC1来维持肿瘤生长。
PNAS:中科院院士叶玉如发现新蛋白IL
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PNAS:中科院院士叶玉如发现新蛋白IL由香港科技大学和英国格拉斯哥大学的科学家们领导的一项研究发现,一种叫做IL-33的蛋白可以逆转小鼠阿尔茨海默病样的病状和认知功能下降。
这项研究发表在4月18日的《美国国家科学院院刊》(PNAS)上。
中科院院士、香港科技大学叶玉如(Nancy Y Ip)教授,及格拉斯哥大学感染、免疫与炎症研究所的Eddy Liew教授是这项研究的共同领导者。
叶玉如教授主要研究神经营养因子与神经元发育之间的关系。
2004年,因在神经科学领域的重要发现获“世界杰出女科学家奖”。
2001年当选为中国科学院院士。
2012年,叶玉如院士领导研究人员证实了Cdk5介导的TrkB磷酸化作用是活动依赖性结构可塑性和空间记忆的必要条件。
这一成果发布在Nature Neuroscience杂志上。
大脑新皮层的功能完整性,依赖于正确数量的兴奋神经元和抑制性神经元。
不过人们并不清楚,新皮层发育过程中的神经元生产异常会导致什么后果。
香港科技大学的叶玉如研究团队发现,新皮层上层的神经元生产过剩会引发类似自闭症的行为,这项研究发表在2014年十一月二十六日的Cell Reports杂志上。
2015年10月,叶玉如院士领导来自中国香港科技大学的研究人员证实,S-亚硝基化(S-nitrosylation)依赖性p35蛋白酶体降解抑制了Cdk5的活性,调控了海马突触强度。
这一研究发现发布Nature Communications杂志上。
阿尔茨海默病是一种破坏性的疾病,当前尚无有效的治疗方法。
它是痴呆最常见的原因,在英国影响了大约85万人,主要是65岁以上的老人。
它影响了这一年龄段1/14的人,并正随着我们的人口老龄化增长。
预计到2030年全球有6500万人将罹患阿尔茨海默病。
Eddy Liew教授说:“阿尔茨海默病当前有着迫切的未满足的临床需求。
我们希望我们的研究结果最终可以转化至人类。
”“机体中各种细胞类型均可生成IL-33,这种蛋白在中枢神经系统(脑和脊髓)中尤为丰富。
f R
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Generalized Sampling:A Variational Approach Jan Kybic,Thierry Blu,and Michael UnserMarch12,2001Biomedical Imaging Group,IOA,DMT Swiss Federal Institute of Technology Lausanne CH-1015Lausanne,SwitzerlandJan.Kybic@epfl.chAbstractWe consider the problem of reconstructing a multi-dimensional and multivariate function f:R m→R n from the discretely and irregularly sampled responses of q linear shift-invariantfilters.Unlike traditional approaches which reconstruct the function in some signal space V,our reconstruction is optimal in the sense of a plausibility criterion J.The reconstruction is either consistent with the measures,or minimizes the consistence error.There is no band-limiting re-striction for the input signals.We show that impor-tant characteristics of the reconstruction process are induced by the properties of the criterion J.We give the reconstruction formula and apply it to several practical cases.1IntroductionWe will deal with the problem offinding a reconstruc-tion f∈F of a multidimensional function f:R m→R n,using a set of samples y ij= k h jk∗∗f k (x i)= h j∗f (x i)from afilter bank H=[h1...h q]sam-pled at N locations x i.The Shannon theory states that a band-limited sig-nal f o can be reconstructed exactly from its regularly spaced ideal samples(h=δ).Papoulis[1]has shown that f o may also be recovered from the output of q linear shift-invariant systems sampled at(1/q)-th theNyquist rate.This theory has been further extendedto multivariate[2](m>1)and multidimensional[3] (n>1)functions.Unser and Zerubia[4]generalized this framework by dropping the band-limiting con-straint.They sought an approximation f in the more general space V(ϕ),generated by integer translates of a functionϕ.Their approximation f is consistentin the sense of producing the same measurements y ij as∗f.Forϕ=sinc,their reconstruction formulas areequivalent to those of Papoulis.We will take a slightly different approach in the present paper.We keep the consistency constraint; we require that f and f be indistinguishable throughour measurement system,i.e., h j∗f (x i)=y ij= h j∗f (x i).However,instead of prescribing a re-construction space V,we seek a solution optimal inthe sense of a plausibility criterion(penalty function) J(f).In other words,we replace the sub-space con-straint f∈V by a variational formulation.The crite-rion J(f)provides the regularization needed to over-come the ambiguity of the reconstruction problem. It may also represent the a priori knowledge in the Bayesian framework,quantifying our confidence that a particular function f is close to the input f.2Variational criterionWe define the solution to the reconstruction prob-lem to be a function f minimizing J(f)under the consistency constraints.Thus,the behavior of the re-construction algorithm is completely described by the criterion J.We will work in a space F of functions for which J is defined.We will assume that J is a semi-norm and can be written as J(f)=B(f,f)1/2, where B is a bilinear form on F.This not only sim-1plifies the subsequent analysis,but also insures the convexity and continuity of the criterion,which im-plies that all local minima are also global minima. As J is a semi-norm,not a norm,there is a kernel K⊆F for which J(f)=0.It can be shown that if two functions f1,f2solve the reconstruction prob-lem,then f1−f2∈K.The bilinearity of B also makes the superposition principle applicable on the reconstruction process:a solution corresponding to a linear combination of sampling values corresponds to the same linear combination of solutions.In the multidimensional case(n>1),it is usually desirable that all components be treated equally.Then the su-perposition principle implies invariance with respect to the rotation of the sampled values,and any other linear operation on them.It can be shown that the invariance properties of the semi-norm J and thefilter bank H translate di-rectly to the invariance properties of the reconstruc-tion problem solution.For example,if the value of J(f)and remains unchanged when f is subject to translation and rotation,then translating or rotat-ing the sampling points results in a solution which is a translation or rotation of the original solution, provided that the sampling is also translation and rotation invariant.In fact,instead of demanding complete invariance,it is enough to have a pseudo-invariance,where J(f)is allowed to be transformed by an arbitrary increasing function independent of f,such as multiplied by a constant.This greatly simplifies the task of having a scale change invari-ant reconstruction problem,because creating a scale pseudo-invariant J is straightforward,while truly scale-invariant J does not exist,except in the trivial cases.Finally,in many applications,we do not want to penalize linear polynomials,as they correspond to the purest form of the solution.2.1Proposed criterionIs there a criterion corresponding to all the above mentioned requirements?The simplest one in the univariate/unidimensional case(m=n=1)is the criterion proposed by Duchon[5]:J(f)= ∂2f/∂x2 2d x 1/2with a corresponding bilinearformB(f,g)= (∂2f/∂x2)(∂2g/∂x2)d xFor arbitrary higher m and n this generalizes asB(f,g)= n i=1 j k=2∂2f i∂x j11···∂x j m m d x J(f)=∂x j11···∂x j m mg i d x= g T∆2f d x(2) A large class of translation invariant bilinear forms can be(under suitable restrictions on f)expressed as B= U∗f,g ,where U is an n×n-matrix of m-variate distributions,a convolutional kernel of the bilinear form[6].In our case U=∆∗∆def=∆2, with Fourier transformˆU= ω 4I.For example for n=m=1and n=1,m=2,we have U=δ(IV)and U=δ(IV)(x1)δ(x2)+2δ′′(x1)δ′′(x2)+δ(IV)(x2)δ(x1), respectively,whereδis Dirac’s mass distribution cen-tered at0andδ′′,δ(IV)are its second and fourth derivatives.3Explicit solutionA solution to the reconstruction problem is given by a remarkably simple formula:f(x)=p(x)+Ni=1qj=1λijϕj(x−x i)(3) 2It consists of two parts.Thefirst part(p)belongs tothe kernel K.If it has a countable basis,we can write it as p(x)= k a k p k(x).It does not contribute to the criterion(J(p)=0),so we intuitively see thatit is useful to accommodate as much as possible of f in this part.The second part consists of a lin-ear combination of generating functionsϕj shifted to all the sampling points.There are q generating functions,where q is the number of samplingfilters h.In the case of regularly spaced x i and q=1,we recover the solution of Unser and Zerubia[4],pro-vided that we use a criterion corresponding to the adequate generating functionϕ.For q>1,we have a multi-wavelet-like representation with several basis functions.As a consequence of the minimization process,thesolution f must satisfyB(f,g)= ijλij h j∗g (x i)for any g∈F(4)where g is an arbitrary variation around the opti-mal f andλij a Lagrange multiplier corresponding to a sample y ij.It is enough to consider g only from among test functions,assuming that the test func-tions are dense in F,which is normally the case.This justifies our earlier restriction.Provided thatϕj are fundamental solutions as de-tailed in the next section,the condition(4)translates into two sets of constraints.Thefirst set makes f sat-isfy the interpolation conditions y ij= h j∗f (x i). This leads to qN equations.The second set must en-sure that the criterion J(f)is defined,that is,f must belong to F.This implies an orthogonality condition B(f,k)=0,for all k from the kernel K.If we know its basis,then also B(f,p k)=0for all p k.There-fore,the second set contains dim K equations,which makes qN+dim K equations for as many unknown coefficientsλij and a k.For the criterion J from(1),the second set of con-straints implies that the second derivatives of f are square integrable.Therefore,they tend to zero(on the average,not necessarily pointwise)towards infin-ity and thus f tends to a linear polynomial.3.1Generating functionsThe generating functionsϕj are fundamental solu-tions satisfying∀g∈F;B(ϕj,g)= h j∗g (0).In the distributional setting,this condition translates to U∗ϕj=h j.This corresponds toˆUˆϕj=ˆh j,pro-vided that the Fourier transformsˆU,ˆϕj andˆh j exist. More concisely,in the matrix form,we get U∗Φ=H andˆUˆΦ=ˆH,whereΦ=[ϕ1...ϕq].It can be inferred that rotationally invariant semi-norms correspond to radial kernels U which in turn lead to radial generating functionsϕ,provided that thefilters h j are also radial.We can then write ϕ(x)=ρ( x )=ρ(r).Fundamental solutions for the iterated Laplacian kernel∆2and h=δare well-known and can be found taking the inverse Fourier transform of ω 4(we are omitting some technical details here).For n=1and m=1,2,3we getρ=r3,ρ=r2log r,andρ=r, respectively,neglecting the multiplicative constant. In the multidimensional case,as the components are treated equally,we simply use the same function for all components,i.e.Φ=ρ(r)I.We have found the fundamental solutionϕδfor ideal(zero-order)sampling h=δ.For other sam-plers,we have simplyϕ=ϕδ∗h.3.2Approximation problemWhen the measures are not exact(for example cor-rupted by noise),it might be more appropriate to drop the consistency constraints and minimize in-stead a weighted sum of a plausibility criterion J p and some measure of the consistency error,that is, the difference between the desired and actual sampled values.The approximation problem then consists of minimizingJ a(f)=J p(f)+ ij d ij y ij,z ij ;z ij= h j∗f (x i)where d is a suitable distance measure.As the con-sistency error depends only on z ij,the minimization min f J(f)can be decomposed as min{zij}min f J(f). The inner minimization is equivalent to the recon-struction problem we have solved already.Therefore3the solution to the approximation problem has also the form(3).Let us now consider an approximation problem cre-ated by adding a least-squares consistency error mea-sure to a criterion J2(f)from(1):J a(f)=J2(f)+γ ij(z ij−y ij)2By standard variational technique wefind that the solution f must verifyB(f,g)=γ ij(z ij−y ij) h j∗g (x i)∀g∈F Comparing this equation with(4),wefind a linear set of equationsλij=γ(z ij−y ij)=γ k a k h j∗p k (x i)++γ klλkl h j∗ϕl (x i−x k)−γy ijwhich permits us,together with the orthogonality constraints B(f,p k)=0,to determineλij and a i.4Examples4.1Reconstruction from irregularsamplesLet us consider the problem offinding a function f: R→R,passing through afinite number of points (x i,y i)and minimizing a criterion J(f)= (f′′)2d x. We have seen that the corresponding bilinear form is B(f,g)= f′′g′′d x with kernel U=δ(IV).The fundamental solution is proportional to|x|3,which can be localized(convolved with a discretefilter)to obtain a cubic B-spline.The reconstruction is thusf(x)=a0+a1x+Ni=1λi|x−x i|3which has N+2unknown parameters.The second derivative is f′′(x)=6 iλi(x−x i).This leads to orthogonality conditions λi=0and λi x i=0,Figure1:Interpolation from function values. because if either of them were not satisfied we would have lim x→±∞f′′=0and consequently f∈F.The remaining N equations come from the consistency conditions f(x i)=y i.The results by Micchelli[7] imply that for distinct points,there is always a unique solution.An example of a reconstruction result is shown in Figure1.4.2Derivative samplingLet us add derivative constraints y′i=f′(x i)to the preceding example.The samplingfilters will then be H= δδ′ .Thefirst fundamental solution corre-sponding to h1=δremainsϕ1=|x|3.The secondone,corresponding to h2=δ′,is obtain by convolving ϕ1with h2which givesϕ2=|x|x.The reconstruction formula is thusf(x)=a0+a1x++Ni=1λi,1|x−x i|3+λi,2|x−x i|(x−x i)The2N+2unknown parameters can be determined from2N consistency equations f(x i)=y i and y′i= f′(x i)and two orthogonality conditions λi,1=0 and λi,2−3λi,1x i=0.An example of reconstruc-tion from derivative sampling is shown in Figure2.45101520251234567Figure 2:Interpolation from function values and derivatives.4.3Reconstruction consistent withLaplace equationThe problem treated in [8]by numerical integration—which we shall be able to solve explicitly here—consists of reconstructing a function from R 3→R while minimizing the norm of the 3D Laplacian oper-ator J ′(f )2= ∆f 2d x .The problem is ill-posed without additional constraints,because the kernel K ′is too big,permitting an infinity of solutions with zero cost.To avoid the ambiguity,we impose f ∈F .We then minimize the criterion (1),because for f ∈F ,the two criteria are equivalent.As expected,the so-lution will have the formf (x )=a 0+3 j =1a i x i +N i =1λi x −x i (5)where x =(x 1,x 2,x 3),with auxiliary conditionsλi =0, λi x i,1=0,and λi x i,2=0.5ConclusionsWe can reconstruct arbitrary multidimensional and multivariate functions from sampled outputs of an arbitrary filter bank.Unlike previous methods ([1–3]),our approach handles irregular sampling,does not impose band-limiting constraints,the solution isoptimal in the sense of a variational criterion,can be made invariant to translations,rotations and scale changes,implicitly specifies the reconstruction space,and is usable also for noisy measurements.This comes at the cost of slightly more involved compu-tation and less numerical stability.References[1] A.Papoulis,“Generalized sampling expansion,”IEEETransactions Circ.Syst.,vol.24,pp.652–654,1977.[2]J.L.Brown Jr.and K.Sangsari,“Sampling reconstructionof n -dimensional band-limited images after multi-linear fil-tering,”IEEE Transactions Circ.Syst.,pp.1035–1038,July 1989.[3] D.Seidner,M.Feder, D.Cubanski,and S.Blackstock,“Introducing to vector sampling expansions,”IEEE Signal Process.Lett.,vol.5,pp.115–117,May 1998.[4]M.Unser and J.Zerubia,“A generalized sampling theorywithout band-limiting constraints,”IEEE Transactions on Circuits and Systems—II:Analog and digital signal pro-cessing ,vol.45,pp.959–969,Aug.1998.[5]J.Duchon,“Splines minimizing rotation-invariant semi-norms in Sobolev spaces,”in Constructive Theory of Func-tions of Several Variables (W.Schempp and K.Zeller,eds.),(Berlin),pp.85–100,Springer-Verlag,1977.[6]L.Schwartz,Th´e orie des Distributions .Paris,France:Hermann,1966.in French.[7] C. A.Micchelli,“Interpolation of scattered data:Dis-tance matrices and conditionally positive definite func-tions,”Constr.Approx.,no.2,pp.11–22,1986.[8]J.Maltz,R.De Mello Koch,and A.Willis,“Reproducingkernel Hilbert space method for optimal interpolation of potential field data,”IEEE Transactions on Image Pro-cessing ,vol.7,Dec.1998.5。
毒力基因调控蛋白PrfA在单核细胞增生李斯特菌生物被膜形成中的作用及分子..
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为了探明太湖流域城市内河冬季功能微生物数量与水质变化的关系,以常州市市区正在生物修复的城市内河—北市河为研究对象,对河流上中下游5个断面的15个采样点,从2012年1至6月进行水样中4种可培养功能微生物(总异养菌、氨化细菌、硝化细菌和磷细菌)数量的测定,并对9项水环境指标(化学需氧量、总磷、总氮、氨氮、硝态氮、温度、溶解氧、pH和叶绿素)进行监测。
结果表明:总异养细菌数量具有明显的时间变化趋势,温度变化对细菌的生长繁殖影响很大,温度高微生物繁殖越快,温度低微生物数量明显减少;氨化细菌数量保持在同一数量级,变化不大。
从进水断面至下游出水断面,分布略呈倒U型变化。
毒力基因调控蛋白PrfA在单核细胞增生李斯特菌生物被膜 形成中的作用及分子机制罗勤周青春冯飞飞张强冯晓琴尚俊丽瞿汇萍华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,武汉 430079 生物被膜(biofilm)可定义为:细菌在生长过程中,为适应生存环境而吸附于惰性或活性材料表面形成的一种与浮游细胞相对应的生长方式。
它由细菌和自身分泌的多糖、蛋白质等胞外基质组成,是一个三维立体空间结构的生态系。
研究表明生物被膜显著增强了微生物对各种杀菌剂的耐受能力(生物被膜菌对抗生素的敏感程度只有浮游菌的1/10~1/1000),耐热性也相应增加,对环境变化不敏感。
在食品生产、加工、运输和保藏过程中,一旦发生病原菌污染并形成生物被膜便难以将其彻底清除,不仅会对各种加工设备和输送管道的接触表面造成损伤,导致热传递效率降低、能耗增加,而且是极易产生食品生物危害的潜在污染源,严重威胁食品卫生安全。
向日葵菌核病拮抗菌的筛选与鉴定郑计梅王海龙张雪芳张建丽北京理工大学,生命学院,北京,100081 菌核病是一种世界性普遍发生的真菌病害,也是向日葵最重要的病害之一。
向日葵菌核病菌属子囊菌亚门、核盘菌属,菌核黑色,鼠粪状,寄主植物非常广泛,除主要危害向日葵和油菜外,还危害十字花科、豆科等植物,寄主植物已达70多科450余种。
《2024年基于生物信息学发现肝细胞癌标志性miRNA及作用与机制研究》范文
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《基于生物信息学发现肝细胞癌标志性miRNA及作用与机制研究》篇一一、引言肝细胞癌(Hepatocellular Carcinoma,HCC)是一种常见的恶性肿瘤,其发病率和死亡率均较高。
早期诊断和有效治疗是降低肝细胞癌患者死亡率的关键。
近年来,随着生物信息学和分子生物学技术的不断发展,越来越多的研究表明,microRNA (miRNA)在肿瘤的发生、发展和转移中起着重要作用。
因此,本研究旨在基于生物信息学技术,发现肝细胞癌标志性miRNA,并研究其作用与机制,为肝细胞癌的早期诊断和治疗提供新的思路和方法。
二、材料与方法1. 材料本研究使用的肝细胞癌组织样本和正常肝组织样本均来自某大型医院。
此外,还使用了公共数据库中的相关数据。
2. 方法(1)生物信息学分析利用生物信息学技术,对公共数据库中的肝癌相关miRNA 表达谱进行分析,筛选出差异表达的miRNA。
然后,利用生物信息学软件对筛选出的miRNA进行功能预测和靶基因预测。
(2)实验验证通过实时荧光定量PCR技术,对筛选出的标志性miRNA在肝细胞癌组织样本和正常肝组织样本中的表达情况进行验证。
同时,利用细胞实验和动物实验,研究该miRNA在肝细胞癌发生、发展和转移中的作用与机制。
三、结果1. 生物信息学分析结果通过生物信息学分析,我们筛选出了一批差异表达的miRNA,其中某个miRNA在肝细胞癌组织中的表达显著高于正常肝组织。
进一步的功能预测和靶基因预测表明,该miRNA可能参与肝细胞癌的发生、发展和转移。
2. 实验验证结果实时荧光定量PCR技术验证结果表明,该标志性miRNA在肝细胞癌组织中的表达确实显著高于正常肝组织。
细胞实验和动物实验结果表明,该miRNA能够促进肝细胞癌细胞的增殖、迁移和侵袭,同时抑制肿瘤细胞的凋亡。
进一步的研究表明,该miRNA通过调控相关信号通路,影响肝细胞癌的发生、发展和转移。
四、讨论本研究基于生物信息学技术,发现了肝细胞癌标志性miRNA,并通过实验验证了其在肝细胞癌发生、发展和转移中的作用与机制。
插值核函数
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插值核函数1. 定义插值核函数(Interpolation Kernel Function)是一种用于插值的数学函数。
插值是一种通过已知数据点来估计未知数据点的方法,常用于信号处理、图像处理、数值分析等领域。
插值核函数是在插值过程中使用的一种数学工具,它可以根据已知数据点的位置和数值,推断出未知数据点的近似数值。
2. 用途插值核函数主要用于以下几个方面:2.1 插值最常见的用途就是进行插值。
在信号处理中,我们经常需要从离散采样的数据中恢复连续信号。
通过使用插值核函数,我们可以根据已有的离散数据点,推断出连续信号在其他位置上的近似数值。
2.2 数据平滑插值核函数也可以用于对数据进行平滑处理。
当原始数据存在噪声或者不规则波动时,通过使用合适的插值核函数,我们可以对这些噪声进行平滑,并得到更加平稳和连续的曲线。
2.3 图像处理在图像处理中,我们经常需要对图像进行放大或缩小操作。
通过使用插值核函数,在缩放过程中可以通过已知像素点的位置和灰度值,推断出新像素点的灰度值,从而实现图像的平滑放大或缩小。
2.4 数值分析插值核函数在数值分析中也有广泛的应用。
例如,在数值积分中,我们需要将连续函数近似为离散数据点,并计算这些离散数据点的加权和来估计积分结果。
插值核函数可以帮助我们根据已知数据点的位置和数值,推断出未知数据点的近似数值,从而实现数值积分。
3. 工作方式插值核函数通常具有以下特点:3.1 局部性插值核函数通常是局部性的,即只有在离未知数据点比较近的已知数据点附近才有较大权重。
这是因为在插值过程中,我们更关注附近数据点对未知数据点的影响,而对远离未知数据点的已知数据点则不太关注。
3.2 权重衰减插值核函数通常会随着距离增加而衰减权重。
这是因为距离未知数据点较远的已知数据点对于估计未知数据点的影响应该较小。
3.3 归一化插值核函数通常需要满足归一化条件,即插值核函数在所有已知数据点处的权重之和为1。
这是为了保证插值结果的准确性和稳定性。
一个新的玉米Bt2基因突变体的遗传分析和分子鉴定
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作物学报 ACTA AGRONOMICA SINICA 2022, 48(3): 572−579/ ISSN 0496-3490; CN 11-1809/S; CODEN TSHPA9E-mail: zwxb301@本研究由国家自然科学基金项目(U1804235, 31771800)和河南农业大学科技创新基金项目(KJCX2020A04)资助。
This study was supported by the National Natural Science Foundation of China (U1804235, 31771800) and the Science and Technology Innovation Fund of Henan Agricultural University (KJCX2020A04).*通信作者(Corresponding author): 陈洪宇, E-mail: chenhongyu@第一作者联系方式: E-mail: 158********@Received (收稿日期): 2021-01-19; Accepted (接受日期): 2021-06-16; Published online (网络出版日期): 2021-07-19. URL: https:///kcms/detail/11.1809.S.20210716.1506.004.htmlDOI: 10.3724/SP.J.1006.2022.13005一个新的玉米Bt2基因突变体的遗传分析和分子鉴定徐宁坤 李 冰 陈晓艳 魏亚康 刘子龙 薛永康 陈洪宇* 王桂凤河南农业大学农学院 / 省部共建小麦玉米作物学国家重点实验室, 河南郑州 450002摘 要: 玉米籽粒发育调控机制的研究对于玉米产量与品质性状的遗传改良十分重要。
本研究鉴定了一个新的转座子插入的籽粒皱缩突变体5601Q , 遗传分析表明其籽粒缺陷稳定遗传且为单基因隐性突变。
社会等级的神经环路机制--神经所胡海岚博士最新Science文章
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生物通报道:来自中科院上海生科院神经科学研究所的研究人员发表了题为“Bidirectional Control of Social Hierarchy by Synaptic Efficacy in Medial Prefrontal Cortex”的文章,报道了通过基因水平的操作调节小鼠特定脑区的活性,从而实现对小鼠行为的调控,揭开社会等级的神经环路机制的最新成果。
这一研究成果公布在Science杂志上。
生物通文章的通讯作者是神经科学研究所的胡海岚研究员,胡海岚博士早年毕业于北京大学生物系,后赴美于加州大学伯克利分校获得博士学位,2008年加入中国科学院神经科学研究所,担任神经环路与行为可塑性研究组组长。
其课题组主要从事可塑性的细胞与环路机制研究。
参与研究的其他人员包括博士生汪菲,朱鸿和研究助理朱军,张琪。
这项研究获得科技部“973”项目、中国科学院“百人计划”,神经科学国家重点实验室以及上海浦江计划,上海择优人才计划等资助,在中科院神经科学研究所独立完成。
生物通社会等级是动物世界中最显著的社会行为之一,也是动物社会的基本组织方式。
对群体而言,稳定的等级结构可以减少族群内不必要的激烈冲突和能量浪费。
对个体而言,在社会等级中的地位深刻地影响其健康和生活质量。
社会等级地位甚至被认为是健康状况的最佳风向标。
然而到目前为止,人们对决定社会等级地位的神经环路机制却知之甚少。
在这项研究中,胡海岚研究组通过改变大脑内侧前额叶(medial prefrontal cortex)的突触传递首次探究了这一环路在社会等级中的重要作用。
通过巧妙地利用钻管测验和其它几种行为测试,研究者首先排列出了群养小鼠的社会等级。
有趣的是,在钻管测验中迫使对方先退出的小鼠和低等级对手相比往往能得到更多的食物、标记更大的领地,并且对雌性小鼠唱出更多的求偶歌曲。
偶尔,高等级小鼠甚至会扮演“剃须师”的角色,将同笼同伴的胡须拔去。
生物通通过电生理记录高等级和低等级鼠的脑片,博士生汪菲发现社会等级地位和小鼠的内侧前额叶神经元突触强度密切相关,即小鼠的社会等级越高,它的内侧前额叶神经元的突触(神经元之间相互通讯连接的节点)强度就越强。
人胰腺癌相关粘蛋白核芯多肽的提取与特性
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人胰腺癌相关粘蛋白核芯多肽的提取与特性马运国;袁玫;李力;费丽华;金永轼【期刊名称】《中国肿瘤生物治疗杂志》【年(卷),期】1996(3)3【摘要】应用分子筛层析及氯化铯密度梯度离心从人胰腺癌细胞株SW1990提取、纯化获得粘蛋白,再用氟氢酸去除粘蛋白的碳水化合物而获得核芯肽。
对粘蛋白核芯肽进行了氨基酸组份分析,并以免疫印迹及ELISA法进行免疫原分析,结果表明所提取的核芯肽至少含有Muc-1,Muc-2,及Muc-3三种不同氨基酸序列的多肽,免疫印迹表明此核芯肽含有从28至大于90KD的多条肽。
在此基础上,制备了抗此核芯肽的单克隆抗体,5株单克隆抗体与多种癌细胞起反应,但多不与正常组织起反应,仅一株单克隆抗体MY-5与部分正常组织反应。
这为进一步观察癌相关核芯肽病理生理功能打下了基础。
【总页数】5页(P178-182)【关键词】胰腺癌;粘蛋白;核芯多肽;提取;特性【作者】马运国;袁玫;李力;费丽华;金永轼【作者单位】解放军总医院肿瘤生物学研究室;美国旧金山医学院【正文语种】中文【中图分类】R735.9【相关文献】1.粘液核芯多肽MUC—2的特性,组织分布及对腺癌的鉴别诊断 [J], 袁玫;金永轼2.自动蛋白印迹仪在检测抗可提取核抗原多肽抗体谱中的应用 [J], 钟丽民;方忠俊;周丽萍;邹广珠3.蝎毒多肽提取物体外抑制胰腺癌细胞侵袭转移及相关机制 [J], 张力冰;张维东;王朝霞;张月英;王兆朋;贾青4.建立胰腺癌黏液蛋白核芯肽-连续重复序列核酸疫苗的实验研究 [J], 吴文川;靳大勇;秦新裕;楼文晖;王单松;倪晓凌;吴肇汉5.粘蛋白核芯肽MUC_1的基因结构及其在肿瘤生物治疗中的意义 [J], 邢英琦;朱迅因版权原因,仅展示原文概要,查看原文内容请购买。
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∇4I = I + ∇2I
(8)
In this new light, equation (7) may be perceived as a more generalized version of Mach’s model, that implies a computation of still higher order derivatives as a linear combination of lower order ones. Kernels derived from such combinations of derivatives by using finite difference
(5)
Therefore using (3), ∇8G(r) = mδ (r) + n∇2G(r) + ∇4G(r) (6)
0-7695-3059-1/07 $25.00 © 2007 IEEE
159
DOI 10.1109/ADCOM.2007.90
So for any intensity distribution I , for an insignificantly wide smoothing function i.e. σ → 0 , from equation (6) we may
Sandip Sarkar1, Kuntal Ghosh2, Kamales Bhaumik3 1Microelectronics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata-700064, India
kamales.bhaumik@wbut.ac.in
Abstract Post processing of medical images often needs interpolation. Taking cues from human visual system, we propose here an interpolation kernel consisting of linear combination of Gaussians at different scales. We compare the efficacy of the proposed kernel with other interpolation kernels, particularly in the processing of medical images. The basic algorithm has been implemented on a TI DM642 based hardware platform for realtime filtering and programmed for post-processing of ultrasound video frames (20fames/s) from the commercially available Siemens Medical Ultrasound Scanner.
ቤተ መጻሕፍቲ ባይዱ
This means, the fourth order derivative can in fact be expressed as a linear combination of two second order derivative operators. Again, following similar procedure it has also been shown [6]:
15th International Conference on Advanced Computing and Communications
A Bio-inspired Interpolation Kernel for Medical Image Processing Implemented on DSP Processor
write:
∇8I = I + n∇2I + ∇4I
(7)
Whereas by taking m = 1 , in equation (1) (here σ / → 0 ), we
may in the same way arrive at Mach’s model [1] in a new light:
sandip.sarkar@saha. ac.in 2Center for Soft Computing Research, Indian Statistical Institute, 203 B. T. Road, Kolkata-700108, India
kuntal_v@isical.ac.in 3 West Bengal University of Technology, BF-142 Salt Lake, Kolkata-700064, India
the Laplacian of Gaussian or the LOG ( ∇2G ) operator, where G is a zero-mean Gaussian, whose standard diviation will later be denoted by σ . In this paper we propose a new multi-scale model of HVS, that generalizes the above-mentioned findings in order to compute general even order rotationally-symmetric Gaussian derivatives and combinations thereof, that yield a new class of filters for the purpose of edge detection and image enhancement. In this paper, the usefulness of such higher order Gaussian derivatives as interpolation kernel would be investigated. Use of interpolation kernels consisting of combinations of Gaussian and its partial derivatives was first proposed by Appledorn [3]. He exploited the near ideal spatial and frequency domain behavior of those filters. Such kernels are locally compact in the space and have excellent frequency domain characteristics. We propose, in this paper a novel
∇4G(r,σ ) = m∇2G(r,σ / ) + ∇2G(r,σ // ) (1)
In the post processing of medical images, the Laplacian based edge detection and image enhancement is a well-known technique. This approach is a direct consequence of the psychophysical findings of Mach [1], who pointed out that the derivatives of light intensity form the basis of human visual system (HVS) at the retinal level. Marr and Hildreth [2] used the Laplacian operator along with a Gaussian smoothing operation for the purpose of edge detection. This was termed as
Key Words: multi-scale Gaussian; image interpolation; realtime processing; DSP processor
I. INTRODUCTION
approach for the generation of interpolation kernel from the linear combinations of Gaussians at different scales taking inspiration from a theorem of Ma and Li [4], that expresses the sum of (n/2+1) Gaussians of different scales as the nth order derivative of a Gaussian function.
(a)
(b)
Interpolation kernels are, generally symmetrical and separable,
so that h(x, y) = h(x) ⋅ h( y). Considering the 1-D case we
can generally write a kernel with a linear combination of n Gaussians as:
(4)
As in the previous deductions [5, 6], if we assume that the
Laplacian of Gaussian in Equation (4) is computed at a very