外周血5-羟色胺水平检测在冠心病诊断中的临床价值及其与氧化应激的关系

外周血5-羟色胺水平检测在冠心病诊断中的临床价值及其与
氧化应激的关系
李陶;庞琪;刘永斌
【期刊名称】《中国卫生标准管理》
【年(卷),期】2016(7)22
【摘要】Objective To investigate the clinical significance of peripheral serotonin in the diagnose of coronary artery disease(CAD)and its relationship with oxidative stress.Methods 96 subjects with cardiovascular risk and suspected CAD were enroled in the present study. According to the CT and angiography results,al subjects were divided into two group including CAD group and non-CAD group. The venous blood samples were colected and whole blood(WB),poor-plate plasma (PPP) and serum samples were prepared. Using High performance liquid chromatography detected the serotonin level in PPP and WB samples. To detected the reactive oxygen metabolites(d-ROM),a Diacron- reactive oxygen metabolites kit was uesed.ResultsCompared with the patients without CAD,the PPP serotonin level in patients with CAD was
higher(P<0.05). Both unvariate and multivariate analysis indicated that higher PPP/WB serotonin ratio was a risk factor for CAD. The d-ROM was higher in patients with CAD(P<0.05). The PPP serrotonin level and PPP/WB serotonin ratio were proportional to d-ROM level in CAD patients. Receiver operating curves showed that the AUG of PP/WB serotonin ratio was 0.658
in the diagnose of CAD. However,after combined with d-ROM level,the AUG could reach in the diagnose of CAD(AUG=0.762).Conclusion Peripheral serotonin could be used as a biomarker in risk estimation in CAD and also has clinical diagnose value. There is a association between peripheral serotonin and oxidative stress. Combining with the oxidative stress parameter,the diagnose value of peripheral serotonin would be imporved.%目的：探讨外周血5-羟色胺水平在冠心病诊断中的临床价值及其与氧化应激水平的关系。

方法研究共纳入于兰州石化总医院就诊的具有心血管疾病风险并考虑为冠心病的门诊患者96例，根据影像学检查结果，将研究对象分为冠心病组及非冠心病组。

采集研究对象静脉血标本，制备全血标本（WB）、乏血小板血浆标本（PPP）及血清标本。

高效液相色谱法检测全血标本、乏血小板血浆标本中的5-羟色胺水平。

利用Diacron-活性氧代谢产物检测试剂盒检测血清活性氧代谢产物（d-ROM）水平。

结果冠心病组研究对象的PPP中的5-羟色胺水平高于非冠心病组，且差异具有统计学意义（P＜0.05），高PPP/WB 5-羟色胺比值是冠心病发病的危险因素。

冠心病组研究对象的血清d-ROM高于非冠心病组，且差异具有统计学意义（P＜0.05）。

冠心病研究对象PPP中的5-羟色胺水平及PPP/WB 5-羟色胺比值均与血清d-ROM水平呈正比。

受试工作者曲线提示，PPP/WB 5-羟色胺比值诊断冠心病的AUG为0.658，联合PPP/WB 5-羟色胺比值及血清d-ROM水平诊断冠心病的效能更高（AUG为0.762）。

结论外周血5-羟色胺水平可用于评估冠心病发病的风险，并具有诊断价值。

冠心病患者的外周血5-羟色胺水平与氧化应激水平有关，联合氧化应激指标后其诊断价值更高。

【总页数】4页(P148-151)
【作者】李陶;庞琪;刘永斌
【作者单位】兰州石化总医院心血管内科，甘肃兰州730060;兰州石化总医院呼吸科，甘肃兰州730060;兰州石化总医院心血管内科，甘肃兰州730060
【正文语种】中文
【中图分类】R541.4
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