白藜芦醇通过诱导细胞周期阻滞及细胞凋亡增强宫颈癌细胞的化疗敏感性
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(1.西京学院，陕西西安710123；2.西安医学院第二附属医院，陕西西安719000)
摘要：«的 探索白藜芦醇(resveratrol)对宫颈癌细胞化疗敏感性的影响及其调控机制。沁 Hela/DDP细胞分为
4 组：Hela/DDP 组，resveratrol 组，顺钳(cisplatin, CDDP)组,resveratrol + CDDP 组。CCK-8 检测细胞活力及增殖。 流式细胞仪检测细胞周期及细胞凋亡。Western blot检测p21、CDK2、cyclinDl、caspase_9、caspase_3、Ecl-2和Eax表 达。结果 Hela/DDP细胞对CDDP的敏感性低于Hela细胞(PVO. 01)。白藜芦醇可增强H ela/DDP细胞对CDDP 的敏感性(P<0. 01)o白藜芦醇可提高CDDP对H ela/DDP细胞增殖的抑制作用(P<0. 01)o白藜芦醇可增强 CDDP 对 Hela/DDP 细胞 G1 期阻滞作用(P<0. 01)。resveratrol 组和 CDDP 组 p21 表达高于 Hela/DDP 组，CDK2 和 cyclinDl 低于 Hela/DDP 组(P<0. 01) 0 与 CDDP 组相比,resveratrol+CDDP 组 P21 表达升高，CDK2 和 cyclinDl 表达降低(P<0. 01)o白藜芦醇可提高CDDP对Hela/DDP细胞凋亡的诱导作用(P<0. 01)o resveratrol组和 CDDP 组 caspas&3、caspasw9 和 Bax表达高于 Hela/DDP 组，Ecl-2 表达低于 Hela/DDP 组(P<0. 01)o 与 CDDP 组 相比,resveratrol+CDDP组caspase-3caspase-9和Bax表达升高，Ecl-2表达下降(P<C0. 01) o结论 白藜芦醇可能 通过诱导细胞周期阻滞及细胞凋亡而增强宫颈癌细胞的化疗敏感性。
关键词：宫颈癌；白藜芦醇;化疗敏感性;细胞周期阻滞;细胞凋亡
中图分类号:R737.3
文献标志码：A
DOI： 10. 7652/jdyxb2O19O5O14
Resveratrol elevates the chemosensitivity of cervical cancer cells by inducing cell cycle arrest and apoptosis
第40卷第5期 2 0 1 9年9月
西安交通大学学报(医学版)
Journal of Xi'an Jiaotong University (Medical Sciences)
Vol. 40 No. 5细胞凋亡 增强宫颈癌细胞的化疗敏感性
岳小丁1,李秀娟2,刘青1,杨捷玲1
YUE Xiao-ding1, LI Xiu-juan2, LIU Qing1, YANG Jie-ling1 (1. Xijing University, Xi‘ an 710123； 2. The Second Affiliated Hospital of
Xi'an Medical College? Xi' an 719000, China)
ABSTRACT: Objective To explore the effect of resveratrol on the chemosensitivity of cervical cancer cells and
its regulatory mechanism. Methods Hela/DDP cells were divided into four groups: Hela/DDP group, resveratrol group, CDDP group, and resveratrol+CDDP group. Cell viability and proliferation were detected by CCK-8. Cell cycle and apoptosis were tested by flow cytometry. The expressions of p21, CDK2, cyclinDl, caspase-9, caspase-3, Bel 2 and Bax were measured by Western blot. Results The sensitivity of Hela/DDP cells to cisplatin was lower than that of Hela cells (PVO. 01). Resveratrol significantly enhanced Hela/DDP cells9 sensitivity to cisplatin (PV 0.01). The inhibition of CDDP on the proliferation of Hela/DDP cells was increased by resveratrol (PVO. 01). The G1 phase arrest of CDDP on Hela/DDP cells was enhanced by resveratrol (PVO. 01). The expression of p21 was higher in resveratrol group and CDDP group than in Hela/DDP group, and the expressions of CDK2 and CyclinDl were lower than in Hela/DDP group (PVO. 01). Compared with CDDP group, the expression of p21 in resveratrol + CDDP group was elevated with the decrease in CDK2 and cyclinDl expressions (PVO. 01). The induction of CDDP on the apoptosis of Hela/DDP cells was elevated by resveratrol (P<C0.01). The expressions of caspase-3, caspase-9 and Bax were higher in resveratrol group and CDDP group than in Hela/DDP group, and the expression of Bcl-2 was lower than in Hela / DDP group (PVO.01). Compared with CDDP group, apoptosis in
摘要：«的 探索白藜芦醇(resveratrol)对宫颈癌细胞化疗敏感性的影响及其调控机制。沁 Hela/DDP细胞分为
4 组：Hela/DDP 组，resveratrol 组，顺钳(cisplatin, CDDP)组,resveratrol + CDDP 组。CCK-8 检测细胞活力及增殖。 流式细胞仪检测细胞周期及细胞凋亡。Western blot检测p21、CDK2、cyclinDl、caspase_9、caspase_3、Ecl-2和Eax表 达。结果 Hela/DDP细胞对CDDP的敏感性低于Hela细胞(PVO. 01)。白藜芦醇可增强H ela/DDP细胞对CDDP 的敏感性(P<0. 01)o白藜芦醇可提高CDDP对H ela/DDP细胞增殖的抑制作用(P<0. 01)o白藜芦醇可增强 CDDP 对 Hela/DDP 细胞 G1 期阻滞作用(P<0. 01)。resveratrol 组和 CDDP 组 p21 表达高于 Hela/DDP 组，CDK2 和 cyclinDl 低于 Hela/DDP 组(P<0. 01) 0 与 CDDP 组相比,resveratrol+CDDP 组 P21 表达升高，CDK2 和 cyclinDl 表达降低(P<0. 01)o白藜芦醇可提高CDDP对Hela/DDP细胞凋亡的诱导作用(P<0. 01)o resveratrol组和 CDDP 组 caspas&3、caspasw9 和 Bax表达高于 Hela/DDP 组，Ecl-2 表达低于 Hela/DDP 组(P<0. 01)o 与 CDDP 组 相比,resveratrol+CDDP组caspase-3caspase-9和Bax表达升高，Ecl-2表达下降(P<C0. 01) o结论 白藜芦醇可能 通过诱导细胞周期阻滞及细胞凋亡而增强宫颈癌细胞的化疗敏感性。
关键词：宫颈癌；白藜芦醇;化疗敏感性;细胞周期阻滞;细胞凋亡
中图分类号:R737.3
文献标志码：A
DOI： 10. 7652/jdyxb2O19O5O14
Resveratrol elevates the chemosensitivity of cervical cancer cells by inducing cell cycle arrest and apoptosis
第40卷第5期 2 0 1 9年9月
西安交通大学学报(医学版)
Journal of Xi'an Jiaotong University (Medical Sciences)
Vol. 40 No. 5细胞凋亡 增强宫颈癌细胞的化疗敏感性
岳小丁1,李秀娟2,刘青1,杨捷玲1
YUE Xiao-ding1, LI Xiu-juan2, LIU Qing1, YANG Jie-ling1 (1. Xijing University, Xi‘ an 710123； 2. The Second Affiliated Hospital of
Xi'an Medical College? Xi' an 719000, China)
ABSTRACT: Objective To explore the effect of resveratrol on the chemosensitivity of cervical cancer cells and
its regulatory mechanism. Methods Hela/DDP cells were divided into four groups: Hela/DDP group, resveratrol group, CDDP group, and resveratrol+CDDP group. Cell viability and proliferation were detected by CCK-8. Cell cycle and apoptosis were tested by flow cytometry. The expressions of p21, CDK2, cyclinDl, caspase-9, caspase-3, Bel 2 and Bax were measured by Western blot. Results The sensitivity of Hela/DDP cells to cisplatin was lower than that of Hela cells (PVO. 01). Resveratrol significantly enhanced Hela/DDP cells9 sensitivity to cisplatin (PV 0.01). The inhibition of CDDP on the proliferation of Hela/DDP cells was increased by resveratrol (PVO. 01). The G1 phase arrest of CDDP on Hela/DDP cells was enhanced by resveratrol (PVO. 01). The expression of p21 was higher in resveratrol group and CDDP group than in Hela/DDP group, and the expressions of CDK2 and CyclinDl were lower than in Hela/DDP group (PVO. 01). Compared with CDDP group, the expression of p21 in resveratrol + CDDP group was elevated with the decrease in CDK2 and cyclinDl expressions (PVO. 01). The induction of CDDP on the apoptosis of Hela/DDP cells was elevated by resveratrol (P<C0.01). The expressions of caspase-3, caspase-9 and Bax were higher in resveratrol group and CDDP group than in Hela/DDP group, and the expression of Bcl-2 was lower than in Hela / DDP group (PVO.01). Compared with CDDP group, apoptosis in