淫羊藿素体外抗淋巴瘤细胞增殖效应

淫羊藿素体外抗淋巴瘤细胞增殖效应
范双翼;余英豪
【期刊名称】《中国比较医学杂志》
【年(卷),期】2011(21)6
【摘要】Objective To investigate the effect and probable mechanism of icaritin (ICT) on EL-4 cells in vitro.Methods MTT、transmission electron microscope (TEM) 、flow cytometry analysis were used to examine the effect of ICT on the proliferation of EL-4 cells.Reverse transcript-polymerase chain reaction (RT-PCR) assay and colorimetric method was used to reveal the probable mechanism.Results ICT could inhibit the proliferation of EL-4 cells significantly in a dose-and time-dependent manner.Apoptotic cells were observed by TEM and flow cytometry analysis.RT-PCR analysis showed that the expression of bcl-2、 P21 were down-regulated.Besides , Caspase-3 and Caspase-9 activity in EL-4 cells elevated remarkably.Conclusions ICT could inhibit the proliferation of EL-4 cells and induce EL-4 cells apoptosis in vitro.The probable mechanism may be down-regulate the expression of bcl-2、P21and elevate Caspase-3 and Caspase-9 activity in EL-4 cells.%目的本实验以小鼠T细胞淋巴瘤细胞株EL-4细胞株为模型,研究淫羊藿素(icaritin,ICT)是否抑制瘤细胞增殖,并初步探讨其作用机制.方法应用MTT比色法、透射电镜技术及流式细胞术检测ICT对EL-4细胞增殖能力的影响;用RT-PCR技术、比色法分析ICT的作用机制.结果 ICT对EL-4细胞增殖具有明显抑制作用,并呈量效及时效关系.透射电镜及流式细胞术结果显
示,ICT可诱导EL-4细胞凋亡.RT-PCR显示,ICT作用于EL-4细胞后,bcl-2、P21
基因的mRNA表达下调,Caspase-3、Caspase-9酶活性显著增强.结论 ICT可抑
制体外培养的小鼠T淋巴瘤EL-4细胞的增殖并诱导其凋亡,可能系通过下调bcl-2、P21 mRNA表达,激活Caspase-3、Caspase-9蛋白等途径实现的.
【总页数】7页(P6-11,后插1)
【作者】范双翼;余英豪
【作者单位】南京军区福州总医院病理科,福州350025;南京军区福州总医院病理科,福州350025
【正文语种】中文
【中图分类】R36;R332
【相关文献】
1.淫羊藿总黄酮及淫羊藿素体外抗骨肉瘤的研究 [J], 刘玉亮;梁广胜;魏强强;何丁文;殷明;殷嫦嫦
2.淫羊藿苷及其代谢产物脱水淫羊藿素促血管内皮细胞增殖的初步机制研究 [J],
赵曙炎;马泽云
3.淫羊藿苷及脱水淫羊藿素对人牙髓细胞增殖和成矿能力的影响 [J], 赵曙炎;马泽云
4.淫羊藿苷与脱水淫羊藿素的体外抗氧化研究 [J], 包宇;杨建雄;孙润广
5.淫羊藿提取物IC163对K562白血病细胞增殖抑制和凋亡诱导效应观察 [J], 朱
剑锋;赵娜;孟坤;张广森
因版权原因，仅展示原文概要，查看原文内容请购买。